Functional analysis of effector of Vibrio parahaemolyticus TTSS2 on enterotoxicity.
| Project/Area Number |
21790419
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
KODAMA Toshio Osaka University, 微生物病研究所, 助教 (20346133)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 腸炎ビブリオ / 腸管毒性 / 3型分泌装置 / エフェクター / エフェクー |
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| Research Abstract |
Many Gram-negative bacterial pathogens use a specialized Type III secretion system (TTSS) to inject virulence factors, called effectors, directly into eukaryotic cells. T3SS2, a TTSS of Vibrio parahaemolyticus which is a pathogen causing food-borne disease worldwide, is indispensable for pathogenicity, and especially enterotoxicity, of this bacterium. However, which effector(s) is or are responsible for the T3SS2-dependent enterotoxicity has remained an open question. In this study, we identified a novel effector, VopE, as a major contributor to the enterotoxicity of Vibrio parahaemolyticus in the rabbit ileal loop test. The VopE protein consists of three domains, the N-terminal, the long repeat (LR), and the C-terminal domains. The LR domain contains three types of repeat units. We demonstrated that VopE directly binds to F-actin via a repeat unit (rep1) in the LR or the C-terminal domain, and that the enterotoxic activity generated by VopE correlated significantly with its F-actin binding activity. We further demonstrated that a TTSS2-related TTSS and a vopE homologous gene in a non-O1/non-O139 V. cholerae strain, which is pathogenic for humans, were also involved in the enterotoxicity of the strain. These results indicate that VopE is a novel F-actin-targeting effector and its ability to bind to F-actin is involved in the enterotoxic activity of TTSS2-possessing pathogens.
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Report
(3 results)
Research Products
(34 results)
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[Journal Article] Two Regulators of Vibrio parahaemolyticus Play Important Roles in Enterotoxicity by Controlling the Expression of Genes in the Vp-PAI Region.2010
Author(s)
Kodama T., Gotoh K., Hiyoshi H., Morita M., Izutsu K., Akeda Y, Park, K.S,. Cantarelli, V.V., Dryselius R., Iida T., Honda T.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli.2009
Author(s)
Ogura Y., Ooka T., Iguchi A., Toh H., Asadulghani M., Oshima K., Kodama T., Abe H., Nakayama K., Kurokawa K., Tobe T., Hattori M., Hayashi T.
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Journal Title
Proc Natl Acad Sci USA. 106
Pages: 17939-17944
Related Report
Peer Reviewed
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[Journal Article] Identification and characterization of a type III secretion-associated chaperone in the type III secretion system 1 of Vibrio parahaemolyticus.2009
Author(s)
Akeda, Y., Okayama, K., Kimura, T., Dryselius, R., Kodama, T., Iida, T., Honda, T.
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Journal Title
FEMS Microbiology Letters 296
Pages: 18-25
Related Report
Peer Reviewed
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[Journal Article] Identification and characterization of a novel type III secretion system in trh-positive Vibrio parahaemolyticus strain TH3996 reveal genetic lineage and diversity of pathogenic machinery beyond the species level.2009
Author(s)
Okada, N., Iida, T., Park, KS., Goto, N., Yasunaga, T., Hiyoshi, H., Matsuda, S., Kodama, T., Honda, T.
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Journal Title
Infection and Immunity 77
Pages: 904-913
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Peer Reviewed
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[Presentation] Identification and characterization of a novel type III secretion system in trh-positive Vibrio parahaemolyticus strain TH3996 reveal genetic lineage and diversity of pathogenic machinery beyond the species level.2009
Author(s)
Okada, N., Iida, T., Park, KS., Goto, N., Yasunaga, T., Hiyoshi, H., Matsuda, S., Kodama. T., Honda, T.
Organizer
US-Japan cooperative medical science program -c holera and other bacterial enteric infections
Place of Presentation
San Diego, USA
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