Analysis of the interaction between enteropathogenic Escherichia coli infection and mucosal immunity in vitro and in vivo

• Project/Area Number: 21790428
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Keio University
• Principal Investigator: NAGAI Takeshi Keio University, 医学部, 助教 (60418655)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 病原細菌 / 免疫 / 細菌感染 / 腸管免疫 / 微生物 / 免疫学 / 細菌

Research Abstract

The aim of this study is to investigate the interaction between enteropathogenic E.coli (EPEC) and host mucosal immune response. Consequently, we found that Peyer's patch was important for bacterial colonization in C57BL/6 mouse but not Balb/c mouse. And then we performed the in vitro infection model using bonemarrow derived dendritic cells and epithelial cells. Consequently, type III secretion system of EPEC directly or indirectly regulated the expression of various genes on dendritic cells.

Research Products

• [Journal Article] Listerial invasion protein internalin B promotes entry into ileal Peyer's patches in vivo. (2011)
  • Author(s): Chiba S, Nagai T, Hayashi T, Baba Y, Nagai S, Koyasu S.
  • Journal Title: Microbiol Immunol vol.55 Pages: 123-129
  • NAID: 10031121450
  • Peer Reviewed
• [Journal Article] Asmall-molecule inhibitor of the bacterial type III secretion system protects against in vivo infection with Citrobacter rodentium. (2011)
  • Author(s): Kimura K, Iwatsuki M, Nagai T, Matsumoto A, Takahashi Y, Shiomi K, Omura S, Abe A.
  • Journal Title: J Antibiot vol.64 Pages: 197-203
  • Peer Reviewed
• [Journal Article] A small-molecule inhibitor of the bacterial type III secretion system protects against in vivo infection with Citrobacter rodentium. (2011)
  • Author(s): Kimura K, Iwatsuki M, Nagai T, Matsumoto A, Takahashi Y, Shiomi K, Omura S, Abe A.
  • Journal Title: J Antibiot Volume: 64 Pages: 197-203
  • NAID: 10030638108
  • Peer Reviewed
• [Journal Article] Listerial invasion protein internalin B promotes entry into ileal Peyer's patches in vivo. (2011)
  • Author(s): Chiba S, Nagai T, Hayashi T, Baba Y, Nagai S, Koyasu S.
  • Journal Title: Microbiol Immnnol Volume: 55 Pages: 123-129
  • NAID: 10031121450
  • Peer Reviewed
• [Journal Article] EspJ effector in enterohemorrhagic E. coli translocates into host mitochondria via an atypical mitochondrial targeting signal. (2010)
  • Author(s): Kurushima J, Nagai T, Nagamatsu K, Abe A
  • Journal Title: Microbiol Immunol vol.54 Pages: 371-379
  • NAID: 10027847815
  • Peer Reviewed
• [Journal Article] EspJ effector in enterohemorrhagic E.coli translocates into host mitochondria via an atypical mitochondrial targeting signal. (2010)
  • Author(s): Kurushima J, Nagai T, Nagamatsu K, Abe A.
  • Journal Title: Microbiol Immunol Volume: 54 Pages: 371-379
  • Peer Reviewed
• [Journal Article] Bacteria hijack integrin-linked kinase to stabilize focal adhesions and block cell detachment. (2009)
  • Author(s): Kim M, Ogawa M, Fujita Y, Yoshikawa Y, Nagai T, Koyama T, Nagai S, Lange A, Fassler R, Sasakawa C
  • Journal Title: Nature vol.459 Pages: 578-582
  • Peer Reviewed
• [Journal Article] Bacteria hijack integrin-linked kinase to stabillze focal adhesions and block cell detachment (2009)
  • Author(s): Kim M, Ogawa M, Fujita Y, Yoshikawa Y, Nagal T, Koyama T, Nagal S, Lange A, Fassler R, Sasakawa O
  • Journal Title: Nature 459 Pages: 578-582
  • Peer Reviewed
• [Presentation] 腸管病原性大腸菌に対する宿主免疫応答の解析 (2010)
  • Author(s): 永井武
  • Organizer: 細菌学会関東支部 インターラボセミナー
  • Place of Presentation: 北里大学感染制御科学府2F講義室
  • Year and Date: 2010-11-26
• [Presentation] 腸管病原性大腸菌に対する宿主免疫応答の解析 (2010)
  • Author(s): 永井武、阿部章夫、渡邉峰雄、小安重夫
  • Organizer: 細菌学会関東支部インターラボセミナー
  • Place of Presentation: 北里大学感染制御学府
  • Year and Date: 2010-11-26