Newly Identification and Analysis of Entry Receptor of Varicella-Zoster Virus

• Project/Area Number: 21790445
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: SUENAGA Tadahiro Osaka University, 微生物病研究所, 助教 (20396675)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 水痘帯状疱疹ウイルス(VZV) / グリコプロテイン / エントリー / 膜融合 / 神経組織指向性 / 単純ヘルペスウイルス(HSV) / MAG / Non-muscle myosin IIA / 組織指向性 / 水痘帯状疱疹ウィルス(VZV) / 神経指向性 / 単純ヘルペスウィルス(HSV)

Research Abstract

We found that MAG expressing specifically in nerve tissues associates with glycoprotein B (gB) of varicella-zoster virus (VZV) and functions as a receptor for VZV during infection. VZV infection of MAG-expressing cells can be inhibited with anti-MAG antibody and with some smaller molecules than antibody.
We also found that MAG is a receptor of herpes simplex virus (HSV) during infection of nerve tissues.

Research Products

• [Journal Article] Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-1 (2010)
  • Author(s): Arii, J., Goto, H., Suenaga, T., Oyama, M.,Kozika-Hata, H., Imai, T., Minowa, A., Akashi, H., Arase, H., Kawaoka, Y., Kawaguchi, Y.
  • Journal Title: Nature 467 Pages: 859-862
  • Peer Reviewed
• [Journal Article] Single Amino Acid Substitution in Herpes simplex virus 1 Envelope Glycoprotein B at a Site Required for Binding to the Paired Immunoglobulin-like Type 2 Receptor α (PILRα) Abrogates PILRα-dependent Viral Entry and Reduces Pathogenesis (2010)
  • Author(s): Arii, J., Wang, J., Morimoto, T., Suenaga, T., Akashi, H., Arase, H., Kawaguchi, Y.A
  • Journal Title: J.Virol. 84 Pages: 10773-10783
  • Peer Reviewed
• [Journal Article] Myelin-associated glycoprotein mediates membrane fusion and entry of neurotropic herpesviruses (2010)
  • Author(s): Suenaga T, Satoh T, Somboonthum P, Kawaguchi Y, Mori Y, Arase H.
  • Journal Title: Proc.Natl.Acad.Sci.USA 107 Pages: 866-871
  • Peer Reviewed
• [Journal Article] Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-1. (2010)
  • Author(s): Arii, J., Goto, H., Suenaga, T., Oyama, M., Kozika-Hata, H., Imai, T., Minowa, A., Akashi, H., Arase, H., Kawaoka, Y., Kawaguchi, Y.
  • Journal Title: Nature Volume: 467 Pages: 859-862
  • Peer Reviewed
• [Journal Article] A Single Amino Acid Substitution in Herpes simplex virus 1 Envelope Glycoprotein B at a Site Required for Binding to the Paired Immunoglobulin-like Type 2 Receptor α(PILRα) Abrogates PILRα-dependent Viral Entry and Reduces Pathogenesis. (2010)
  • Author(s): Arii, J., Wang, J., Morimoto, T., Suenaga, T., Akashi, H., Arase, H., Kawaguchi, Y.
  • Journal Title: J.Virol. Volume: 84 Pages: 10773-10783
  • Peer Reviewed
• [Journal Article] Myelin-associated glycoprotein mediates membrane fusion and entry of neurotropic herpesviruses (2010)
  • Author(s): Suenaga T, Satoh T, Somboonthum P, Kawaguchi Y, Mori Y, Arase H
  • Journal Title: Proceedings of the National Academy of Sciences USA 107 Pages: 866-871
  • Peer Reviewed
• [Presentation] 神経組織指向性αヘルペスウイルスのエントリーレセプター (2010)
  • Author(s): 末永忠広、荒瀬尚
  • Organizer: 第58回日本ウイルス学会(シンポジウム)
  • Place of Presentation: 徳島県郷土文化会館(徳島県)
  • Year and Date: 2010-11-09
• [Presentation] Interaction between Siglec and Varicella-Zoster Virus (VZV) (2010)
  • Author(s): Tadahiro Suenaga, Fuminori Arisawa, Pranee Somboonthum, Yasuko Mori, HisashiArase.
  • Organizer: 第14回国際免疫学会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-08-24
• [Presentation] Myelin-Associated Glycoprotein Associates with gB and is Involved in Membrane Fusion during Neurotropic Herpesvirus Infection (2010)
  • Author(s): Tadahiro Suenaga, Fuminori Arisawa, Takeshi Satoh, Pranee Somboonthum, Yasuko Mori, Yasushi Kawaguchi, Hisashi Arase.
  • Organizer: 35th International Herpesvirus Workshop
  • Place of Presentation: Salt Lake City, Utah (USA)
  • Year and Date: 2010-07-25
• [Presentation] Interaction between Siglec and Varicella-Zoster Virus (VZV) (2010)
  • Author(s): Tadahiro Suenaga, Pranee Somboonthum, Yasuko Mori, Hisashi Arase
  • Organizer: IFReC-CSI joint symposium
  • Place of Presentation: Hangzhou (China)
• [Presentation] 新規神経指向性ヘルペスウイルスレセプターの同定と解析 (2010)
  • Author(s): 末永忠広、荒瀬尚
  • Organizer: 第51回日本神経学会総会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] αヘルペスウイルス感染における神経組織指向性レセプター (2010)
  • Author(s): 末永忠広、有澤史倫、佐藤毅史、Pranee Somboonthum、森康子、川口寧
  • Organizer: 第25回ヘルペスウイルス研究会
  • Place of Presentation: カリアック(浜松市)
• [Presentation] Interaction between Siglec and Varicella-Zoster Virus (VZV) (2010)
  • Author(s): Tadahiro Suenaga, Fuminori Arisawa, Pranee Somboonthum, Yasuko Mori, Hisashi Arase.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸国際展示場
• [Presentation] 神経組織指向性αヘルペスウイルスのエントリーレセプター (2010)
  • Author(s): 末永忠広、荒瀬尚
  • Organizer: 第58回日本ウイルス学会(シンポジウム)
  • Place of Presentation: 徳島県郷土文化会館
• [Presentation] 水痘帯状疱疹ウイルス(VZV)新規エントリーレセプター (2009)
  • Author(s): 末永忠広、有澤史倫、Pranee Somboonthum、森康子、荒瀬尚
  • Organizer: 第57回日本ウイルス学会
  • Place of Presentation: 都市センターホテル(東京都)
  • Year and Date: 2009-10-26
• [Presentation] 水痘帯状疱疹ウィルス(VZV)新規エントリーレセプター (2009)
  • Author(s): 末永忠広、有澤史倫、Pranee Somboonthum、森康子、荒瀬尚
  • Organizer: 第57回日本ウィルス学会
  • Place of Presentation: 都市センターホテル(東京都)
  • Year and Date: 2009-10-26
• [Remarks] ホームページ等
  • URL: http://immchem.biken.osaka-u.ac.jp/
• [Remarks]
  • URL: http://immchem.biken.osaka-u.ac.jp/
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/topics.php?year=2010#2010-Arase-PNAS866