Study on the mechanism of hepatitis C virus infection-induced apoptosis

• Project/Area Number: 21790446
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Kobe University
• Principal Investigator: DENG LIN Kobe University, 大学院・医学研究科, グローバルCOE研究員 (40437497)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: C型肝炎ウイルス / アポトーシス / ミトコンドリア / Bax / JNK

Research Abstract

In this study, by using the J6/JFH1 strain of HCV and Huh7.5 cells, I demonstrated that HCV-induced ROS production triggers JNK activation, which leads to Bax activation and apoptosis. I also found that HCV core plays a role in HCV-induced apoptosis.

Research Products

• [Journal Article] Sequence heterogeneity of NS5A and core proteins of hepatitis C virus and virological responses to pegylated-interferon/ribavirin combination therapy. (2011)
  • Author(s): El-Shamy A, Shoji I, Saito T, Watanabe H, Ide YH, Deng L, Kawata S, Hotta H.
  • Journal Title: Microbiol.Immunol. in press
  • NAID: 10031121486
  • Peer Reviewed
• [Journal Article] Polymorphisms of Hepatitis C Virus NS5A and Core Protein and Clinical Outcome of Pegylated-Interferon/ /Ribavirin Combination Therapy. (2011)
  • Author(s): El-Shamy A, Kim SR, Ide YH, Sasase N, Imoto S, Deng L, Shoji I, Hotta H.
  • Journal Title: Intervirology in press
  • Peer Reviewed
• [Journal Article] Sequence heterogeneity of NS5A and core proteins of hepatitis C virus and virological responses to pegylated-interferon/ribavirin combination therapy (2011)
  • Author(s): Ahmed El-Shamy
  • Journal Title: Microbiology and Immunology
  • Peer Reviewed
• [Journal Article] Polymorphisms of hepatitis C virus NS5A and core protein and clinical outcome of Pegylated-Interferon/Ribavirin combination therapy (2011)
  • Author(s): Ahmed El-Shamy
  • Journal Title: Intervirology
  • Peer Reviewed
• [Journal Article] Analysis of neutralizing antibodies against hepatitis C virus in patients who were treated with pegylated-interferon plus ribavirin. (2010)
  • Author(s): Sasayama M, Deng L, Kim SR, Ide YH, Shoji I, Hotta H.
  • Journal Title: Kobe J.Med.Sci. 56(2)
  • NAID: 110007670723
  • Peer Reviewed
• [Journal Article] 17β-estradiol inhibits the production of infectious particles of hepatitis C virus. (2010)
  • Author(s): Hayashida K, Shoji I, Deng L, Jiang DP, Ide YH, Hotta H.
  • Journal Title: Microbiol.Immunol. 54(11) Pages: 684-689
  • NAID: 10029546273
  • Peer Reviewed
• [Journal Article] Secondary structure of the amino-terminal region of HCV NS3 and virological response to pegylated interferon plus ribavirin therapy for chronic hepatitis C. (2010)
  • Author(s): Sanjo M, Saito T, Ishii R, Nishise Y, Haga H, Okumoto K, Ito J, Watanabe H, Saito K, Togashi H, Fukuda K, Imai Y, El-Shamy A, Deng L, Shoji I, Hotta H, Kawata S.
  • Journal Title: J.Med.Virol. 82(8) Pages: 1364-1370
  • Peer Reviewed
• [Journal Article] 17β-estradiol inhibits the production of infectious particles of hepatitis C virus (2010)
  • Author(s): Kazumi Hayashida
  • Journal Title: Microbiology and Immunology Volume: 54 Pages: 684-690
  • Peer Reviewed
• [Journal Article] Secondary structure of the amino-terminal region of HCV NS3 and virological response to pegylated interferon plus ribavirin therapy for chronic hepatitis C (2010)
  • Author(s): Mai Sanjo
  • Journal Title: Journal of Medical Virology Volume: 82 Pages: 1364-1370
  • Peer Reviewed
• [Journal Article] Analysis of neutralizing antibodies against hepatitis C virus in patients who were treated with peglated-Interferon plus ribavirin (2010)
  • Author(s): Mikiko Sasayama
  • Journal Title: The Kobe Journal of Medical Sciences Volume: 56
  • Peer Reviewed
• [Journal Article] The hepatitis C virus core protein contains a BH3 domain that regulates apoptosis through specific interaction with human Mcl-1. (2009)
  • Author(s): Mohd-Ismail NK, Deng L, Sukumaran SK, Yu VC, Hotta H, Tan YJ.
  • Journal Title: J.Virol. 83(19) Pages: 9993-10006
  • Peer Reviewed
• [Journal Article] The hepatitis C virus core protein contains a BH3 domain that regulates apoptosis through specific interaction with human Mcl-1 (2009)
  • Author(s): Nur Khairiah Mohd-Ismail
  • Journal Title: Journal of Virology 83 Pages: 9993-10006
  • Peer Reviewed
• [Presentation] C型肝炎ウイルス感染は転写因子FoxO1を介した糖新生を誘導する (2010)
  • Author(s): Deng Lin、兼田崇作、井出良浩、勝二郁夫、堀田博
  • Organizer: 第63回日本細菌学会関西支部総会
  • Place of Presentation: 枚方
  • Year and Date: 2010-11-20
• [Presentation] 糖代謝に及ぼすC型肝炎ウイルスの影響及びその分子機序の解析 (2010)
  • Author(s): Deng Lin、兼田崇作、井出良浩、勝二郁夫、堀田博
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-07
• [Presentation] 糖代謝に及ぼすC型肝炎ウイルスの影響及びその分子機序の解析 (2010)
  • Author(s): Lin Deng
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島県郷土文化会館(徳島県)
  • Year and Date: 2010-11-07
• [Presentation] HCV-induced generation of reactive oxygen species leads to Bax-mediated apoptosis through activation of the c-Jun NH_2-terminal kinase signaling pathway. (2010)
  • Author(s): Deng L, Ide YH, Shoji I, Hotta H.
  • Organizer: 17^<th> international symposium on hepatitis c virus & related virus
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-09-11
• [Presentation] HCV-induced generation of reactive oxygen species leads to Bax-mediated apoptosis through activation of the c-Jun NH_2-terminal kinase signaling pathway (2010)
  • Author(s): Lin Deng
  • Organizer: 17^<th> international symposium on hepatitis c virus & related virus
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-09-11
• [Presentation] C型肝炎ウイルス感染によるBax活性化の分子機序の解析 (2009)
  • Author(s): Deng Lin、井出良浩、勝二郁夫、堀田博
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-26
• [Presentation] C型肝炎ウイルス感染によるBax活性化の分子機序の解析 (2009)
  • Author(s): Lin Deng
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル(東京都)
  • Year and Date: 2009-10-26
• [Presentation] Activation of JNK, but not p38 MAPK, is involved in HCV-induced, Bax-mediated apoptosis. (2009)
  • Author(s): Deng L, Ide YH, Shoji I, Hotta H.
  • Organizer: 16^<th> international symposium on hepatitis c virus & related virus
  • Place of Presentation: Nice, France
  • Year and Date: 2009-10-04
• [Presentation] Activation of JNK, but not p38 MAPK, is involved in HCV-induced, Bax-mediated apoptosis (2009)
  • Author(s): Lin Deng
  • Organizer: 16^<th> international symposium on hepatitis c virus & related virus
  • Place of Presentation: Nice, France
  • Year and Date: 2009-10-04