Mechanism of Hepatitis C Virus assembly

• Project/Area Number: 21790449
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Chiba Institute of Technology
• Principal Investigator: HISHIKI Takayuki Chiba Institute of Technology, 附属総合研究所, 特別研究員 (30535761)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 分子 / C型肝炎ウイルス / 粒子形成機構 / アポリポタンパク質 / 感染 / アポリポプロテイン

Research Abstract

We found that Hepatitis C virus (HCV) associate with Apolipoprotein E (ApoE) prior to virus release. Moreover, we clarified that ApoE is important factor for HCV infectivity. Infectivity of HCV to cells that were suppressed endogenous LDLR as well as SR-BI was severally reduced. Furthermore, suppression of both the receptors didn't alter the infectivity to cells that were suppressed either one of the receptors. These results suggest that the infectivity of HCV required both LDLR and SR-BI.

Research Products

• [Journal Article] Infectivity of hepatitis C virus is influenced by association with apolipoprotein E isoforms. (2010)
  • Author(s): Hishiki T, Shimizu Y, Tobita R, Sugiyama K, Ogawa K, Funami K, Ohsaki Y, Fujimoto T, Takaku H, Wakita T, Baumert TF, Miyanari Y, Shimotohno K.
  • Journal Title: J Virol. 84(22) Pages: 12048-12057
  • Peer Reviewed
• [Journal Article] Lipoprotein lipase and hepatic triglyceride lipase reduce the infectivity of hepatitis C virus (HCV) through their catalytic activities on HCV-associated lipoproteins. (2010)
  • Author(s): Shimizu Y, Hishiki T, Sugiyama K, Ogawa K, Funami K, Kato A, Ohsaki Y, Fujimoto T, Takaku H, Shimotohno K.
  • Journal Title: Virology. 407(1) Pages: 152-159
  • Peer Reviewed
• [Journal Article] Infectivity of hepatitis C virus is influenced by association with apolipoprotein E isoforms. (2010)
  • Author(s): Hishiki T, Shimizu Y, Tobita R, Sugiyama K, Ogawa K, Funami K, Ohsaki Y, Fujimoto T, Takaku H, Wakita T, Baumert TF, Miyanari Y, Shimotohno K.
  • Journal Title: Journal of Virology Volume: 84 Pages: 12048-12057
  • Peer Reviewed
• [Journal Article] Lipoprotein lipase and hepatic triglyceride lipase reduce the infectivity of hepatitis C virus (HCV) through their catalytic activities on HCV-associated lipoproteins. (2010)
  • Author(s): Shimizu Y, Hishiki T, Sugiyama K, Ogawa K, Funami K, Kato A, Ohsaki Y, Fujimoto T, Takaku H, Shimotohno K.
  • Journal Title: Virology Volume: 407 Pages: 152-159
  • Peer Reviewed
• [Journal Article] Hepatitis C virus utilizes lipid droplet for production of infectious virus. (2009)
  • Author(s): Ogawa K, Hishiki T, Shimizu Y, Funami K, Sugiyama K, Miyanari Y, Shimotohno K.
  • Journal Title: Proc Jpn Acad Ser B Phys Biol Sci. 85(7) Pages: 217-228
  • NAID: 130000138028
  • Peer Reviewed
• [Journal Article] Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro. (2009)
  • Author(s): Sugiyama K, Suzuki K, Nakazawa T, Funami K, Hishiki T, Ogawa K, Saito S, Shimotohno KW, Suzuki T, Shimizu Y, Tobita R, Hijikata M, Takaku H, Shimotohno K.
  • Journal Title: J Virol. 83(13) Pages: 6922-6928
  • Peer Reviewed
• [Journal Article] Hepatitis C virus utilizes lipid droplet for production of infectious virus. (2009)
  • Author(s): Ogawa K, Hishiki T, Shimizu Y, Funami K, Sugiyama K, Miyanari Y, Shimotohno K.
  • Journal Title: Proceedings of the Japan Academy, Series B Vol.85 Pages: 217-228
  • NAID: 130000138028
  • Peer Reviewed
• [Journal Article] Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro. (2009)
  • Author(s): Sugiyama K, Suzuki K, Nakazawa T, Funami K, Hishiki T, Ogawa K, Saito S, Shimotohno KW, Suzuki T, Shimizu Y, Tobita R, Hijikata M, Takaku H, Shimotohno K.
  • Journal Title: Journal of Virology Vol.83 Pages: 6922-6928
  • Peer Reviewed
• [Journal Article] C型肝炎ウイルスの増幅機構 (2009)
  • Author(s): 日紫喜隆行、杉山和夫
  • Journal Title: 細胞 Vol.41 Pages: 8-11
• [Presentation] Hepatitis C Virus Hijacks Lipid Metabolism for its Proliferation ; Likely Underlying Mechanisms for the Development of Hepatocellular Carcinoma. (2011)
  • Author(s): 日紫喜隆行、清水裕子、宮成悠介、杉山和夫、舟見健児、宇治野真之、高久洋、下遠野邦忠
  • Organizer: The 3rd JCA-AACR Special Joint Conference
  • Place of Presentation: 東京ベイ舞浜ホテル(千葉県)
  • Year and Date: 2011-03-02
• [Presentation] Hepatitis C Virus Hijacks Lipid Metabolism for its Proliferation ; Likely Underlying Mechanisms for the Development of Hepatocellular Carcinoma (2011)
  • Author(s): 日紫喜隆行、清水裕子、宮成悠介、杉山和夫、舟見健児、宇治野真之、高久洋、下遠野邦忠
  • Organizer: The 3rd JCA-AACR Special Joint Conference
  • Place of Presentation: 東京ベイ舞浜ホテル(千葉県)
  • Year and Date: 2011-03-02
• [Presentation] C型肝炎ウイルスの感染性におけるアポリポプロテインEの役割. (2010)
  • Author(s): 日紫喜隆行、清水裕子、杉山和夫、舟見健児、宇治野真之、高久洋、下遠野邦忠
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島県)
  • Year and Date: 2010-11-07
• [Presentation] C型肝炎ウイルスの感染性におけるアポリポプロテインEの役割 (2010)
  • Author(s): 日紫喜隆行、清水裕子、杉山和夫、舟見健児、宇治野真之、高久洋、下遠野邦忠
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島県)
  • Year and Date: 2010-11-07
• [Presentation] Apolipoprotein E isoform affects infectivity of HCV. (2010)
  • Author(s): 日紫喜隆行、清水裕子、杉山和夫、舟見健児、宇治野真之、高久洋、下遠野邦忠
  • Organizer: 17^<th> International meeting on HCV
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-09-11
• [Presentation] Apolipoprotein E isoform affects infectivity of HCV (2010)
  • Author(s): 日紫喜隆行、清水裕子、杉山和夫、舟見健児、宇治野真之、高久洋、下遠野邦忠
  • Organizer: 17^<th> International meeting on HCV
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-09-11
• [Presentation] Apolipoprotein E is Required for Infectivity of Hepatitis C Virus. (2009)
  • Author(s): 日紫喜隆行、飛田怜里、清水裕子、小川和也、舟見健児、宮成悠介、大崎雄樹、藤本豊士、高久洋、杉山和夫、下遠野邦忠
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-09
• [Presentation] Apolipoprotein E is Required for Infectivity of Hepatitis C Virus (2009)
  • Author(s): 日紫喜隆行、飛田怜里、清水裕子、小川和也、舟見健児、宮成悠介、大崎雄樹、藤本豊士、高久洋、杉山和夫、下遠野邦忠
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-09
• [Presentation] Apolipoprotein E is Required for Infectivity of Hepatitis C Virus. (2009)
  • Author(s): 日紫喜隆行、飛田怜里、清水裕子、小川和也、舟見健児、宮成悠介、大崎雄樹、藤本豊士、高久洋、杉山和夫、下遠野邦忠
  • Organizer: 16^<th> International symposium on HCV
  • Place of Presentation: Nice(France)
  • Year and Date: 2009-10-26
• [Presentation] Apolipoprotein E is Required for Infectivity of Hepatitis C Virus (2009)
  • Author(s): 日紫喜隆行、飛田怜里、清水裕子、小川和也、舟見健児、宮成悠介、大崎雄樹、藤本豊士、高久洋、杉山和夫、下遠野邦忠
  • Organizer: International symposium on HCV
  • Place of Presentation: Nice(France)
  • Year and Date: 2009-10-26
• [Presentation] C型肝炎ウイルス粒子の感染性とリポタンパク質. (2009)
  • Author(s): 日紫喜隆行、飛田怜里、清水裕子、小川和也、舟見健児、宮成悠介、大崎雄樹、藤本豊士、高久洋、杉山和夫、下遠野邦忠
  • Organizer: 日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル(東京都)
  • Year and Date: 2009-10-04
• [Presentation] C型肝炎ウイルス粒子の感染性とリポタンパク質 (2009)
  • Author(s): 日紫喜隆行、飛田怜里、清水裕子、小川和也、舟見健児、宮成悠介、大崎雄樹、藤本豊士、高久洋、杉山和夫、下遠野邦忠
  • Organizer: 日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル(東京都)
  • Year and Date: 2009-10-04
• [Book] 最新医学(ウイルス肝炎の病態解明と治療の進歩) (2010)
  • Author(s): 日紫喜隆行、清水裕子
  • Publisher: 最新医学社
• [Book] 細胞(C型肝炎ウイルスの増幅機構) (2009)
  • Author(s): 日紫喜隆行、杉山和夫
  • Publisher: ニュー・サイエンス社