Study for the mechanism of HIV-1-specific CTL induction in the intestinal mucosa

• Project/Area Number: 21790453
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TERAHARA Kazutaka National Institute of Infectious Diseases, 免疫部, 研究員 (50469954)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 感染防御 / ワクチン / ウイルス / 免疫学 / サルモネラ / HIV / 腸管

Research Abstract

In this study, we aimed to elucidate the mechanism by which human immunodeficiency virus (HIV)-specific cytotoxic T lymphocytes (CTLs) are induced in the intestine. HIV-1 Gag-expressing Salmonella typhimurium aroA- or aroA-invAmutants, which can or not, respectively, penetrate Peyer's patches, were used as oral antigens into mice. However, Gag-specific CTLs were hardly induced in both the systemic and the gut mucosal compartments. Further study should be needed for better understanding of the role of Peyer's patches in the induction of mucosal CTLs.

Research Products

• [Journal Article] Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress. (2011)
  • Author(s): Terahara K, Nochi T, Yoshida M, Takahashi Y, Goto Y, Hatai H, Kurokawa S, Jang MH, Kweon M-N, Domino SE, Hiroi T, Yuki Y,Tsunetsugu-Yokota Y, Kobayashi K, Kiyono H
  • Journal Title: Biochem. Biophys.Res.Commun. 404 Pages: 822-828
• [Journal Article] Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress (2011)
  • Author(s): Terahara K, Nochi T, Yoshida M, Takahashi Y, Goto Y, Hatai H, Kurokawa S, Jang MH, Kweon M-N, Domino SE, Hiroi T, Yuki Y, Tsunetsugu-Yokota Y, Kobayashi K, Kiyono H.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 404 Pages: 822-828
  • Peer Reviewed
• [Presentation] HIV-1 infection enhances the susceptibility of T cells to measles virus infection by upregulating signaling lymphocyte activation molecule (SLAM) expression. (2011)
  • Author(s): Mitsuki Y, Shibusawa K, Terahara K, Kobayashi K, Takeda M, Yanagi Y, Tsunetsugu-Yokota Y
  • Organizer: Keystone Symposia
  • Place of Presentation: British Columbia, Canada
• [Presentation] Inhibition of HIV-1 replication by short hairpin RNA is augmented by using lentivirus vector pseudotyped with envelope proteins of wild-type measles virus. (2011)
  • Author(s): Shibusawa K, Mitsuki Y, Terahara K, Ishige M, Yanagi Y, Kobayashi K, Tsunetsugu-Yokota Y
  • Organizer: Keystone Symposia
  • Place of Presentation: British Columbia, Canada
• [Presentation] HIV-1 infection enhances the susceptibility of T cells to measles virus infection by upregulating signaling lymphocyte activation molecule (SLAM) expression (2011)
  • Author(s): Mitsuki Y, Shibusawa K, Terahara K, Kobayashi K, Takeda M, Yanagi Y, Tsunetsugu-Yokota Y
  • Organizer: Keystone Symposia
  • Place of Presentation: カナダ(ウィスラー,プリティッシュコロンビア)
• [Presentation] Inhibition of HIV-1 replication by short hairpin RNA is augmented by using lentivirus vector pseudotyped with envelope proteins of wild-type measles virus (2011)
  • Author(s): Shibusawa K, Mitsuki Y, Terahara K, Ishige M, Yanagi Y, Kobayashi K, Tsunetsugu-Yokota Y
  • Organizer: Keystone Symposia
  • Place of Presentation: カナダ(ウィスラー,プリティッシュコロンビア)
• [Presentation] 麻疹ウイルスエンベロープをもちいたHIV-1増殖抑制性レンチウイルスベクターの開発とその有効性 (2010)
  • Author(s): 渋沢謙太郎、光木裕也、寺原和孝、石毛真行、柳雄介、小林和夫、横田(恒次)恭子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] HIV-1感染モデルとしてのヒト化マウスの妥当性とX4およびR5HIV-1感染 (2010)
  • Author(s): 石毛真行、寺原和孝、光木裕也、渋沢謙太郎、小林和夫、岡田誠治、横田(恒次)恭子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] Inhibition of HIV-1 replication by envelope-engineered lentivirus vectors : efficient gene delivery using envelope protein of measles virus. (2010)
  • Author(s): Shibusawa K, Mitsuki Y, Terahara K, Ishige M, Yanagi Y, Kobayashi K, Tsunetsugu-Yokota Y
  • Organizer: The 10th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji, Japan
• [Presentation] Inhibition of HIV-1 replication by engineered lentivirus vectors : a potential advantage of SLAM-mediated virus entry. (2010)
  • Author(s): Shibusawa K, Mitsuki Y, Terahara K, Yanagi Y, Kobayashi K, Tsunetsugu-Yokota Y
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
• [Presentation] HIV-1 infection enhances the susceptibility of T cells to measles virus infection by upregulating signaling lymphocyte activation molecule (SLAM) expression. (2010)
  • Author(s): Mitsuki Y, Shibusawa K, Terahara K, Kobayashi K, Takeda M, Yanagi Y, Tsunetsugu-Yokota Y
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
• [Presentation] Characteristic activation/differentiation phenotype of CD4^+ T cells and their distinct susceptibility to X4-type and/or R5-type HIV-1 infection in humanized NOD/SCID/Jac3-null mice. (2010)
  • Author(s): Terahara K, Ishige M, Mitsuki Y, Shibusawa K, Watanabe S, Okada S, Kobayashi K, Tsunetsugu-Yokota Y
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
• [Presentation] Factors crucial for the preferential propagation of R5-tropic HIV-1 in the early phase of HIV-1 infection. (2010)
  • Author(s): Tsunetsugu-Yokota Y, Terahara K
  • Organizer: The 5th German-Japanese Symposium
  • Place of Presentation: Tokyo, Japan
• [Presentation] HIV-1 infection enhances the susceptibility of T cells to measles virus infection by upregulating signaling lymphocyte activation molecule (SLAM) expression. (2010)
  • Author(s): Mitsuki Y, Shibusawa K, Terahara K, Kobayashi K, Takeda M, Yanagi Y, Tsunetsugu-Yokota Y
  • Organizer: The 5th German-Japanese Symposium
  • Place of Presentation: Tokyo, Japan
• [Presentation] Inhibition of HIV-1 replication by engineered lentivirus vectors : a potential advantage of SLAM-mediated virus entry. (2010)
  • Author(s): Shibusawa K, Mitsuki Y, Terahara K, Yanagi Y, Kobayashi K, Tsunetsugu-Yokota Y
  • Organizer: The 5th German-Japanese Symposium
  • Place of Presentation: Tokyo, Japan
• [Presentation] HIV-1感染モデルとしてのヒト化マウスの妥当性とX4およびR5 HIV-1感染 (2010)
  • Author(s): 石毛真行、寺原和孝、光木裕也、渋沢謙太郎、小林和夫、岡田誠治、横田(恒次)恭子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] Inhibition of HIV-1 replication by envelope-engineered lentivirus vectors : efficient gene delivery using envelope protein of measles virus (2010)
  • Author(s): Shibusawa K, Mitsuki Y, Terahara K, Ishige M, Yanagi Y, Kobayashi K, Tsunetsugu-Yokota Y
  • Organizer: The 10th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路
• [Presentation] Inhibition of HIV-1 replication by engineered lentivirus vectors : a potential advantage of SLAM-mediated virus entry (2010)
  • Author(s): Shibusawa K, Mitsuki Y, Terahara K, Yanagi Y, Kobayashi K, Tsunetsugu-Yokota Y
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] HIV-1 infection enhances the susceptibility of T cells to measles virus in fection by upregulating signaling lymphocyte activation molecule (SLAM) expression (2010)
  • Author(s): Mitsuki Y, Shibusawa K, Terahara K, Kobayashi K, Takeda M, Yanagi Y, Tsunetsugu-Yokota Y
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] Characteristic activation/differentiation phenotype of CD4^<+> T cells and their distinct susceptibility to X4-type and/or R5-type HIV-1 infection in humanized NOD/SCID/Jac3-null mice (2010)
  • Author(s): Terahara K, Ishige M, Mitsuki Y, Shibusawa K, Watanabe S, Okada S, Kobayashi K, Tsunetsugu-Yokota Y
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] Factors crucial for the preferential propagation of R5-tropic HIV-1 in the early phase of HIV-1 infection (2010)
  • Author(s): Tsunetsugu-Yokota Y, Terahara K
  • Organizer: The 5th German-Japanese Symposium
  • Place of Presentation: 東京
• [Presentation] HIV-1 infection enhances the susceptibility of T cells to measles virus infection by upregulating signaling lymphocyte activation molecule (SLAM) expression (2010)
  • Author(s): Mitsuki Y, Shibusawa K, Terahara K, Kobayashi K, Takeda M, Yanagi Y, Tsunetsugu-Yokota Y
  • Organizer: The 5th German-Japanese Symposium
  • Place of Presentation: 東京
• [Presentation] Inhibition of HIV-1 replication by engineered lentivirus vectors : a potential advantage of SLAM-mediated virus entry (2010)
  • Author(s): Shibusawa K, Mitsuki Y, Terahara K, Yanagi Y, Kobayashi K, Tsunetsugu-Yokota Y
  • Organizer: The 5th German-Japanese Symposium
  • Place of Presentation: 東京
• [Remarks] ホームページ等