| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Research Abstract |
Old BALB/c mice infected with SARS-CoV showed the viral clearance and the development of pneumonia during 6-9 dpi. In contrast, young SCID mice were persistently infected with SARS-CoV during experimental periods (by 21 dpi) without pneumonia, indicating that adaptive immune responses are involved in not only the viral clearance but also the development of pneumonia. We depleted CD4^+ and/or CD8+ cells of old BALB/c mice by specific antibodies. On the other hand, the splenocytes of old BALB/c mice were adoptively transferred into young SCID mice. After SARS-CoV infection, we investigated the development of pneumonia in both mouse models. These results suggest that proinflammatory cytokines produced by pneumocytes and immune competent cells except for lymphocytes result in the development of pneumonia in old BALB/c mice.
|
