Elucidation of physiological function of store-operated calcium influx in B cells and its molecular mechanism.

• Project/Area Number: 21790469
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: BABA Yoshihiro Osaka University, 免疫学フロンティア研究センター, 特任准教授 (20415269)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: B細胞 / ストア作動性カルシウム流入 / STIM1 / STIM2

Research Abstract

We established Stim2-deficient DT40 B cells and analyzed the functional difference between STIM1 and STIM2. We generated Stim2-deficient mice, but these are lethal about 4~5 weeks after birth. Furthermore, we found that B cells lacking STIM1 and STIM2 were defective in BCR-evoked NFAT activation and IL-10 production, suggesting that STIM proteins are essential for suppressive function through NFAT-dependent IL-10.

Research Products

• [Journal Article] The Calcium Sensors STIM1 and STIM2 Control B cell Regulatory Function through IL-10 Production. (2011)
  • Author(s): Matsumoto M, Baba Y
  • Journal Title: Immunity Volume: (in press)
  • Peer Reviewed
• [Journal Article] S-glutathionylation activates STIM1 and alters mitochondrial homeostasis. (2010)
  • Author(s): Hawkins BJ, Irrinki KM, Mallilankaraman K, Lien YC, Wang Y, Bhanumathy CD, Subbiah R, Ritchie MF, Soboloff J, Baba Y, Kurosaki T, Joseph SK, Gill DL, Madesh M.
  • Journal Title: J.Cell Biol. 190 Pages: 391-405
  • Peer Reviewed
• [Journal Article] Ca^<2+> signaling and STIM1. (2010)
  • Author(s): Kurosaki T, Baba Y.
  • Journal Title: Prog Biophys Mol Biol. 103 Pages: 51-58
• [Journal Article] B Cell Signaling and Fate Decision. (2010)
  • Author(s): Kurosaki T, Shinohara H, Baba Y.
  • Journal Title: Annu.Rev.Immunol. 28 Pages: 21-55
• [Journal Article] S-glutathionylation activates STIM1 and alters mitochondrial homeostasis. (2010)
  • Author(s): Hawkins BJ, Baba Y
  • Journal Title: J.Cell Biol. Volume: 190 Pages: 391-405
  • Peer Reviewed
• [Journal Article] Ca^<2+> signaling and STIM1. (2010)
  • Author(s): Kurosaki T, Baba Y
  • Journal Title: Prog.Biophys.Mol.Biol. Volume: 103 Pages: 51-58
• [Journal Article] B Cell Signaling and Fate Decision. (2010)
  • Author(s): Kurosaki T, Baba Y
  • Journal Title: Annu.Rev.Immunol. Volume: 28 Pages: 21-55
• [Journal Article] Physiological function and molecular basis of STIM1-mediated calcium entry in immune cells. (2009)
  • Author(s): Baba Y
  • Journal Title: Immunological Review 231 Pages: 174-188
• [Journal Article] STIM protein coupling in the activation of Orai channels. (2009)
  • Author(s): Wang Y
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 7391-7396
  • Peer Reviewed
• [Journal Article] A Stiml-dependent, noncapacitative Ca^<2+>-entry pathway is activated by B-cell-receptor stimulation and depletion of Ca^<2+> (2009)
  • Author(s): Morita T
  • Journal Title: J.Cell Sci. 122 Pages: 1220-1228
  • Peer Reviewed
• [Journal Article] The Calcium Sensors STIM1 and STIM2 Control B cell Regulatory Function through Interleukin-10 Production.
  • Author(s): Matsumoto M, Fujii Y, Baba A, Hikida M, Kurosaki T, Baba Y.
  • Journal Title: Immunity in press
  • Peer Reviewed
• [Presentation] ストア作動性カルシウム流入活性化機構とその生理的役割 (2010)
  • Author(s): 馬場義裕
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール,盛岡(招待講演)
  • Year and Date: 2010-05-19
• [Presentation] B細胞における小胞体カルシウムセンサーSTIMの生理的役割 (2010)
  • Author(s): 馬場義裕
  • Organizer: 第83回日本薬理学会年会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-18
• [Presentation] B細胞における小胞体カルシウムセンサーSTIMの生理的役割 (2010)
  • Author(s): 馬場義裕
  • Organizer: 第83回日本薬理学会年会シンポジウム
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-03-18
• [Presentation] Store-operated Calcium Entry in B cells. (2009)
  • Author(s): 馬場義裕
  • Organizer: 第39回日本免疫学会総会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] Store-operated Calcium Entry in B cells (2009)
  • Author(s): 馬場義裕
  • Organizer: 第39回日本免疫学会総会シンポジウム
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2009-12-03
• [Presentation] Essential role of STIM1, ER calcium sensor, for store-operated calcium influx and mast cell activation. (2009)
  • Author(s): 馬場義裕
  • Organizer: 第10回運動器科学研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-18
• [Presentation] Essential role of STIM1, ER calcium sensor, for store-operated calcium influx and mast cell activation (2009)
  • Author(s): 馬場義裕
  • Organizer: 第10回運動器科学研究会
  • Place of Presentation: 東京ステーションコンファレンス(東京)
  • Year and Date: 2009-09-18
• [Presentation] Physiological function for store-operated calcium entry in immune response. (2009)
  • Author(s): 馬場義裕
  • Organizer: The 2nd International Symposium of WPI-IFReC.第二回国際世界トップレベル拠点免疫学フロンティアセンターシンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-02-12