Functional analysis of molecular mechanism of type I interferon production regulated by ubiquitin ligases

• Project/Area Number: 21790477
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Chiba Institute of Technology
• Principal Investigator: FUNAMI Kenji Chiba Institute of Technology, 附属総合研究所, 研究員 (00421983)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 自然免疫 / ユビキチンリガーゼ / I型インターフェロン

Research Abstract

In my project, I have no progression in the field of TRIM25 function. So I performed the functional analyses of ubiquitin ligases other than TRIM25. From these analyses, I identified the new function of C-terminal region of RNF125, which is ubiquitin ligase regulating type-I interferon production.

Research Products

• [Journal Article] Polyubiquitin conjugation to NEMO by triparite motif protein 23 (TRIM 23) is critical in antiviral defense (2011)
  • Author(s): Arimoto, K., K.Funami, et al.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 107(36) Pages: 15856-61
  • Peer Reviewed
• [Journal Article] Infectivity of hepatitis C virus is influenced by association with apolipoprotein E isoforms. (2010)
  • Author(s): Hishiki, T., Shimizu, Y., Tobita, R., Sugiyama, K., Ogawa, K., Funami, K., Ohsaki, Y., Fujimoto, T., Takaku, H., Wakita, T., Baumert, T.F., Miyanari, Y., Shimotohno, K.
  • Journal Title: J Virol 84(22) Pages: 12048-12057
  • Peer Reviewed
• [Journal Article] Lipoprotein lipase and hepatic triglyceride lipase reduce the infectivity of hepatitis C virus (HCV) through their catalytic activities on HCV-associated lipoproteins. (2010)
  • Author(s): Shimizu, Y., Hishiki, T., Sugiyama, K., Ogawa, K., Funami, K., Kato, A., Ohsaki, Y., Fujimoto, T., Takaku, H., Shimotohno, K.
  • Journal Title: Virology 407(1) Pages: 152-159
  • Peer Reviewed
• [Journal Article] Polyubiquitin conjugation to NEMO by triparite motif protein 23 (TRIM23) is critical in antiviral defense. (2010)
  • Author(s): Arimoto, K., Funami, K., Saeki, Y., Tanaka, K., Okawa, K., Takeuchi, O., Akira, S., Murakami, Y., Shimotohno, K.
  • Journal Title: Proc Natl Acad Sci USA 107(36) Pages: 15856-15861
  • Peer Reviewed
• [Journal Article] Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro. (2009)
  • Author(s): Sugiyama, K., Suzuki, K., Nakazawa, T., Funami, K., Hishiki, T., Ogawa, K., Saito, S., Shimotohno, K.W., Suzuki, T., Shimizu, Y., Tobita, R., Hijikata, M., Takaku, H., Shimotohno, K.
  • Journal Title: J Virology 83(13) Pages: 6922-6928
  • Peer Reviewed
• [Journal Article] Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro. (2009)
  • Author(s): Sugiyama K, et al.
  • Journal Title: Journal of Virology 83(13) Pages: 6922-6928
  • Peer Reviewed
• [Journal Article] Hepatitis C virus utilizes lipid droplet for production of infectious virus. (2009)
  • Author(s): Ogawa K, et al.
  • Journal Title: Proc Jpn Acad Ser B Phys Biol Sci. 85(7) Pages: 217-228
  • Peer Reviewed
• [Presentation] Characterization of functional domain at the C-terminal region of RNF125 (2009)
  • Author(s): 舟見健児、有元啓一郎、日紫喜隆行、清水裕子、小川和也、杉山和夫、下遠野邦忠
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] Characterization of functional domain at the C-terminal region of RNF 125 (2009)
  • Author(s): 舟見健児
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-09