Analysis of immunological synapse in physiological condition

• Project/Area Number: 21790486
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TANE Akiko 独立行政法人理化学研究所, 免疫シグナル研究グループ, 研究員 (10415226)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 免疫シナプス / T 細胞抗原受容体 / 蛍光イメージング / 細胞骨格 / T細胞抗原受容体

Research Abstract

When T cells recognize antigen on antigen.presenting cells, they start adhere and generate T cell antigen receptors (TCR) microclusters (TCR-MCs) which is a unit for starting T cell activation. The TCR-MCs move toward the center of the interface and form immunological synapse. We revealed and published that the transportation of TCR-MCs depends on microtubule motor, dynein and negatively regulate T cell activation. These observation were based on the quantitative analysis of TCR-MCs generated under physiological stimulus conditions.

Research Products

• [Journal Article] Dynein-Driven Transport of T Cell Receptor Microclusters Regulates Immune Synapse Formation and T Cell Activation (2011)
  • Author(s): Akiko Hashimoto-Tane, Tadashi Yokosuka, Kumiko Sakata-Sogawa, Machie Sakuma, Chitose Ishihara, Makio Tokunaga, Takashi Saito
  • Journal Title: Immunity Volume: 34 Pages: 919-931
  • Peer Reviewed
• [Journal Article] T cell receptor-microclusters critical for T-cell activation are formed independently of lipid raft clustering (2010)
  • Author(s): Hashimoto-Tane A, Yokosuka T, Ishihara C, Sakuma M, Kobayashi W and Saito, T
  • Journal Title: Molecular and Cellular Biology Volume: 30 (14) Pages: 3421-3429
  • Peer Reviewed
• [Journal Article] Spatiotemporal basis of CTLA-4 costimulatory molecule-mediated negative regulation of T cell activation (2010)
  • Author(s): Yokosuka, T, Kobayashi, W, Takamatsu, M, Sakata-Sogawa, K, Zeng, H, Hashimoto-Tane, A, Yagita, H, Tokunaga, M, Saito, T
  • Journal Title: Immunity Volume: 33(3) Pages: 326-339
  • Peer Reviewed
• [Journal Article] Dynamic regulation of T cell activation and co-stimulation through TCR-microclusters (2010)
  • Author(s): Saito, T, Yokosuka, T. and Hashimoto-Tane, A
  • Journal Title: FEBS Letters Volume: 584 Pages: 4865-4871
  • Peer Reviewed
• [Journal Article] T cell receptor-microclusters critical for T-cell activation are formed independently of lipid raft clustering. (2010)
  • Author(s): Hashimoto-Tane A, Yokosuka T, Ishihara C, Sakuma M, Kobayashi W, Saito, T
  • Journal Title: Molecular and Cellular Biology Volume: 30(14) Pages: 3421-3429
  • Peer Reviewed
• [Journal Article] Spatiotemporal basis of CTLA-4 costimulatory molecule-mediated negative regulation of T cell activation. (2010)
  • Author(s): Yokosuka, T, Kobayashi, W, Takamatsu, M, Sakata-Sogawa, K, Zeng, H, Hashimoto-Tane, A, Yagita, H, Tokunaga, M, Saito, T
  • Journal Title: Immunity Volume: 33(3) Pages: 326-339
  • Peer Reviewed
• [Journal Article] Dynamic regulation of T cell activation and co-stimulation through TCR-microclusters. (2010)
  • Author(s): Saito, T, Yokosuka, T., Hashimoto-Tane, A
  • Journal Title: FEBS Letters Volume: 584 Pages: 4865-4871
  • Peer Reviewed
• [Presentation] 免疫シナプス形成を担うダイニンによるT 細胞抗原受容体運搬の分子機構 (2012)
  • Author(s): 多根(橋本)彰子、横須賀忠、斉藤隆
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-16
• [Presentation] 免疫シナプス形成を担うダイニンによるT細胞抗原受容体運搬の分子機構 (2012)
  • Author(s): 多根(橋本)彰子
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-16
• [Presentation] モーター分子ダイニンによるT 細胞受容体ミクロクラスター輸送を介した活性化制御 (2012)
  • Author(s): 多根(橋本)彰子
  • Organizer: 第21回東京免疫フォーラム
  • Place of Presentation: 東京
  • Year and Date: 2012-03-08
• [Presentation] モーター分子ダイニンによるT細胞受容体ミクロクラスター輸送を介した活性化制御 (2012)
  • Author(s): 多根(橋本)彰子
  • Organizer: 第21回東京免疫フォーラム
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2012-03-08
• [Presentation] 免疫シナプスにおけるダイニンによるT 細胞ミクロクラスター運搬の分子機構 (2011)
  • Author(s): 多根(橋本)彰子、横須賀忠、斉藤隆
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-29
• [Presentation] 免疫シナプスにおけるダイニンによるT細胞ミクロクラスター運搬の分子機構 (2011)
  • Author(s): 多根(橋本)彰子
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-29
• [Presentation] Dynein-driven transport of TCR microclusters forms Immune synapse and regulates T cell activation (2011)
  • Author(s): Hashimoto-Tane,A., Yokosuka, T., Sakuma, M. and Saito, T
  • Organizer: The 84th Annual Meeting of The Japanese Pharmacology Society
  • Place of Presentation: on line
• [Presentation] Dynein-driven transport of TCR microclusters forms Immune synapse and regulates T cell activation. (2011)
  • Author(s): Hashimoto-Tane, A., Yokosuka, T., Sakuma, M., Saito, T.
  • Organizer: The 84^<th> Annual Meeting of The Japanese Pharmacology Society
  • Place of Presentation: 横浜
• [Presentation] Dynein-driven transport of TCR microclusters forms Immune synapse and regulates T cell activation (2010)
  • Author(s): Hashimoto-Tane,A., Saito, T
  • Organizer: The 16th Takeda Science Foundation Symposium on Bioscience
  • Place of Presentation: 東京
  • Year and Date: 2010-12-01
• [Presentation] Dynein-mediated translocation of TCR microclusters along microtubules to form csmac during T cell activation (2010)
  • Author(s): Hashimoto-Tane, Yokosuka,T., Machie Sakuma, Takashi Saito
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-24
• [Presentation] Dynein-mediated translocation of TCR microclusters along microtubules to form cSMAC Keystone Symposia (2010)
  • Author(s): Hashimoto-Tane,A., Yokosuka, T., Saito, T
  • Organizer: Lymphocyte Activation and Gene Expression
  • Place of Presentation: Breckenridge (USA)
  • Year and Date: 2010-03-01
• [Presentation] Dynein-mediated translocation of TCR microclusters along microtubules to form cSMAC (2010)
  • Author(s): 橋本彰子(多根), 横須賀忠, 斉藤隆
  • Organizer: Keystone Symposia Lymphocyte Activation and Gene Expression
  • Place of Presentation: Breckenridge(USA)
  • Year and Date: 2010-03-01
• [Presentation] Dynein-mediated translocation of TCR microclusters along microtubules to form csmac during T cell activation (2010)
  • Author(s): Hashimoto-Tane, Yokosuka, T., Machie Sakuma, Takashi Saito
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] Dynein-driven transport of TCR microclusters forms Immune synapse and regulates T cell activation (2010)
  • Author(s): Hashimoto-Tane, A., Saito, T.
  • Organizer: The 16^<th> Takeda Science Foundation Symposium on Bioscience
  • Place of Presentation: 東京
• [Presentation] T細胞活性化及びcSMAC形成につながる微小管モーター・ダイニンによるTCRミクロクラスターの移動 (2009)
  • Author(s): 橋本彰子(多根)、横須賀忠、斉藤隆
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] TCR microclusters move along microtubules in early T cell activation (2009)
  • Author(s): Hashimoto-Tane, A., Yokosuka, T.,Ishihara, C. and Saito, T
  • Organizer: The 5th International Workshop of Kyoto T Cell Conference
  • Place of Presentation: Kyoto
  • Year and Date: 2009-06-01
• [Presentation] TCR microclusters move along microtubules in early T cell activation (2009)
  • Author(s): Hashimoto-Tane, A., Yokosuka, T, Ishihara, C., Saito, T.
  • Organizer: The 5^<th> International Workshop of Kyoto T Cell Conference
  • Place of Presentation: Kyoto
  • Year and Date: 2009-06-01
• [Remarks] Immunity preview
  • URL: http://www.sciencedirect.com/science/article/pii/S1074761311002354
• [Remarks] 理化学研究所プレスリリース
  • URL: http://www.riken.jp/r-world/info/release/press/2011/110624_2/detail.html
• [Remarks] ライフサイエンス 新着論文レビュー
  • URL: http://first.lifesciencedb.jp/archives/3201
• [Remarks]
  • URL: http://first.lifesciencedb.jp/archives/3201