Visualization and molecular analysis of antigen-uptake and -delivery at M cell.

• Project/Area Number: 21790487
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KIMURA Shunsuke The Institute of Physical and Chemical Research, 大学院・医学研究科, 助教 (40444525)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: パイエル板 / M細胞 / CCR1 / CCL9 / 形質細胞 / pDC

Research Abstract

Peyer's patch (PP) in the small intestine is a primary site for the intestinal immune system. The luminal side of PPs is covered by the follicle-associated epithelium (FAE), which contains M cells. M-cells uptake antigens or macromolecules and deliver them to dendritic cells inhabiting subepithelial dome region under FAE. We found high expression level of a chemokine gene, Ccl9, in the M cells by using the mouse chemokine array and in situ hybridization analysis. The cells expressing CCR1 protein, a receptor of CCL9, were found underneath M cells and close contact with. Moreover, M cells expressed a gene of M-Sec which product is a key molecule of the formation of membrane nanotubes, a new structure for cell-cell communication. Taken together, our data suggest that M cells interact and communicate with immune cells by two ways, CCL9-CCR1 and membrane nanotubes.

Research Products

• [Journal Article] The epithelia-specific membrane trafficking factor AP-1B secures gut immune homeostasis in mice. (2011)
  • Author(s): Takahashi D, Hase K, Kimura S, Nakatsu F, Ohmae M, Mandai Y, Sato T, Date Y, Ebisawa M, Kato T, Obata Y, Fukuda S, Kawamura Y, Dohi D, Katsuno T, Yokosuka O, Waguri S, Ohno H
  • Journal Title: Gastroenterology
  • Peer Reviewed
• [Journal Article] M-Sec : Emerging secrets of tunneling nanotube formation. (2010)
  • Author(s): Ohno H, Hase K, Kimura S
  • Journal Title: Commun Integr Biol. 3(3) Pages: 231-233
  • Peer Reviewed
• [Journal Article] M-Sec : Emerging secrets of tunneling nanotube formation. (2010)
  • Author(s): Hiroshi Ohno, Koji Hase, Shunsuke Kimura
  • Journal Title: Communicative & Integrative Biology Volume: 3 Pages: 231-233
  • Peer Reviewed
• [Journal Article] M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex. (2009)
  • Author(s): Hase K, Kimura S, Takatsu H, Ohmae M, Kawano S, Kitamura H, Ito M, Watarai H, Hazelett CC, Yeaman C, Ohno H.
  • Journal Title: Nat Cell Biol 11(12) Pages: 1427-1432
  • Peer Reviewed
• [Journal Article] Uptake through glycoprotein 2 of FimH(+) bacteria by M cells initiates mucosal immune response. (2009)
  • Author(s): Hase K, Kawano K, Nochi T, Pontes GS, Fukuda S, Ebisawa M, Kadokura K, Tobe T, Fujimura Y, Kawano S, Yabashi A, Waguri S, Nakato G, Kimura S, Murakami T, Iimura M, Hamura K, Fukuoka S, Lowe AW, Itoh K, Kiyono H, Ohno H.
  • Journal Title: Nature 462(7270) Pages: 226-230
  • Peer Reviewed
• [Journal Article] Monitoring autophagy in mammalian cultured cells through the dynamics of LC3. (2009)
  • Author(s): Kimura S, Fujita N, Noda T, Yoshimori T
  • Journal Title: Methods Enzymol 452 Pages: 1-12
  • Peer Reviewed
• [Journal Article] M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex (2009)
  • Author(s): Hase K, Kimura S, Takatsu H, Ohmae M, Kawano S, Kitamura H, Ito M, Watarai H, Hazelett CC, Yeaman C, Ohno H
  • Journal Title: Nat Cell Biol 11 Pages: 1427-32
  • Peer Reviewed
• [Journal Article] Uptake through glycoprotein 2 of FimH(+)bacteria by M cells initiates mucosal immune response (2009)
  • Author(s): Hase K, Kawano K, Nochi T, Pontes GS, Fukuda S, Ebisawa M, Kadokura K, Tobe T, Fujimura Y, Kawano S, Yabashi A, Waguri S, Nakato G, Kimura S, Murakami T, Iimura M, Hamura K, Fukuoka S, Lowe AW, Itoh K, Kiyono H, Ohno H.
  • Journal Title: Nature 462 Pages: 226-30
  • Peer Reviewed
• [Journal Article] Monitoring autophagy in mammalian cultured cells through the dynamics of LC3 (2009)
  • Author(s): Kimura S, Fujita N, Noda T, Yoshimori T.
  • Journal Title: Methods Enzymol 452 Pages: 1-12
• [Presentation] M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex (2010)
  • Author(s): Koji Hase, Shunsuke Kimura, Hiroshi Ohno
  • Organizer: 第14回国際免疫学会議
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-08-26
• [Presentation] M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex (2010)
  • Author(s): Koji Hase, Shunsuke Kimura, Hiroshi Ohno
  • Organizer: 第14回国際免疫学会議
  • Place of Presentation: 神戸ポートピアホテル、神戸国際展示場(神戸)
  • Year and Date: 2010-08-26
• [Presentation] Functional analysis of CCL9 in small intestine (2009)
  • Author(s): Ryo Yamazaki, Shunsuke Kimura, Masashi Ebisawa, Yasuhiro Date, Satoshi Tsuneda, Haruhiko Suzuki, Koji Hase, Hiroshi Ohno
  • Organizer: 日本免疫学会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2009-12-02
• [Presentation] (2009)
  • Author(s): Shunsuke Kimura, Ayako Sakamoto, Ryo Yamazaki, Kazuya Kawano, Koji Hase, Hiroshi Ohno
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-06-02
• [Presentation] Functional analysis of CCL9 in small intestine (2009)
  • Author(s): Ryo Yamazaki, Shunsuke Kimura, Koji Hase, Masashi Ebisawa, Yasuhiro Date, Hiroshi Ohno
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-06-02