Establishment of the effective cell culture method for regulatory T cells.

• Project/Area Number: 21790535
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Okayama University
• Principal Investigator: SHIBAKURA Misako Okayama University, 大学院・保健学研究科, 准教授 (30314694)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 制御性T細胞 / 培養方法 / P-セレクチン / PSGL-1 / 移植片対宿主病

Research Abstract

I tried the establishment of the effective cell culture method of regulatory T cells. To clarify the role of selectins/selectin-ligands in the cell proliferation and the function of Treg cells, we examined the cell proliferation rate and the function of Treg cells, using P-selectin-/-mice and PSGL-1-/-mice. The growth rate of P-selectin-/-Treg cells and PSGL-1-/-Treg cells were high in comparison with the wild-type Treg cells. PSGL-1-/-Treg cells could inhibit wild-type T cell proliferation in in-vitro suppression assay.