Molecular epidemiological study on the causative agent of cat scratch disease, Bartonella henselae

• Project/Area Number: 21790538
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Yamaguchi University
• Principal Investigator: YANAGIHARA Masashi 山口大学, 大学院・医学系研究科, 助教 (40379954)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 猫ひっかき病 / Cat scratch disease / Bartonella henselae / Multilocus sequencetyping(MLST) / Multispacer typing(MST) / virB5 / bepA / badA / cat scratch disease / Multispacer typing (MST) / 猫引っかき病 / multilocus sequence typing (MLST)

Research Abstract

Bartonella henselae is the causative agent of cat scratch disease(CSD). We characterized B. henselae isolates from CSD patients and cats using DNA sequence-based genotyping methods. Our study demonstrated that human infections in Japan can be caused by B. henselae strains in MST cluster 1, distinct from clusters containing the Houston-1 and Marseille type strains. Furthermore, we developed a novel genotyping method based on three virulence genes(virB5, bepA and badA), and uncovered the genotypic distribution of B. henselae in Japan.

Research Products

• [Journal Article] Prosthetic valve endocarditis caused by Bartonella quintana in a patient during immunosuppressive therapies for collagen vascular diseases (2011)
  • Author(s): Yamada Y, Ohkusu K, Yanagihara M, Tsuneoka H, Ezaki T, Tsuboi J, Okabayashi H, Suwabe A.
  • Journal Title: Diagnostic Microbiology and Infectious Disease Volume: 70 Pages: 395-398
  • Peer Reviewed
• [Journal Article] Oxidation stress index' as a possible clinical marker for the evaluation of non-Hodgkin lymphoma (2011)
  • Author(s): Nojima J, Motoki Y, Tsuneoka H, Kuratsune H, Matsui T, Yamamoto M, Yanagihara M, Hinoda Y, Ichihara K
  • Journal Title: British Journal of Haematology Volume: 155 Pages: 528-530
  • Peer Reviewed
• [Journal Article] 'Oxidation stress index' as a possible clinical marker for the evaluation of non-Hodgkin lymphoma (2011)
  • Author(s): Nojima J
  • Journal Title: British Journal of Haematology Volume: 155 Issue: 4 Pages: 528-530
  • DOI: 10.1111/j.1365-2141.2011.08719.x
  • Peer Reviewed
• [Journal Article] Multispacer Typing of Bartonella henselae Isolates from Humans and Cats, Japan (2010)
  • Author(s): Yanagihara M, Tsuneoka H, Sugasaki M, Nojima J, Ichihara K
  • Journal Title: Emerging Infectious Diseases Volume: 16 Pages: 1983-1985
  • Peer Reviewed
• [Journal Article] Antimicrobial susceptibility by Etest of Bartonella henselae isolated from cats and human in Japan (2010)
  • Author(s): Tsuneoka H, Yanagihara M, Nojima J, Ichihara K
  • Journal Title: Journal of Infection and Chemotherapy Volume: 16 Pages: 446-448
  • NAID: 10027656193
  • Peer Reviewed
• [Journal Article] A first Japanese case of Bartonella henselae induced endocarditis diagnosed by prolonged culture of a specimen from the excised valve (2010)
  • Author(s): Tsuneoka H, Yanagihara M, Otani S, Katayama Y, Fujinami H, Nagafuji H, Asari S, Nojima J, Ichihara K
  • Journal Title: Diagnostic Microbiology and Infectious Disease Volume: 68 Pages: 174-176
  • Peer Reviewed
• [Journal Article] Molecular typing of Bartonella henselae DNA extracted from human clinical specimens and cat isolates in Japan (2010)
  • Author(s): Yanagihara M, Tsuneoka H, Hoshide S, Ishido E, Umeda A, Tsukahara M, Nojima J, Ichihara K, Hino K, Hirai I, Yamamoto Y
  • Journal Title: FEMS Immunology and Medical Microbiology Volume: 60 Pages: 44-48
  • Peer Reviewed
• [Journal Article] Multispacer typing of Bartonella henselae isolates from humans and cats, Japan. (2010)
  • Author(s): Yanagihara M
  • Journal Title: Emerging Infectious Diseases Volume: 16 Pages: 1983-1985
  • Peer Reviewed
• [Journal Article] Molecular typing of Bartonella henselae DNA extracted from human clinical specimens and cat isolates in Japan. (2010)
  • Author(s): Yanagihara M
  • Journal Title: FEMS Immunology and Medical Microbiology Volume: 60 Pages: 44-48
  • Peer Reviewed
• [Journal Article] A first Japanese case of Bartonella henselae induced endocarditis diagnosed by prolonged culture of a specimen from the excised valve. (2010)
  • Author(s): Tsuneoka H
  • Journal Title: Diagnostic Microbiology and Infectious Disease Volume: 68 Pages: 174-176
  • Peer Reviewed
• [Journal Article] Antimicrobial susceptibility by Etest of Bartonella henselae isolated from cats and human inJapan. (2010)
  • Author(s): Tsuneoka H
  • Journal Title: Journal of Infection and Chemotherapy Volume: 16 Pages: 446-448
  • Peer Reviewed
• [Journal Article] Acquired activated protein C resistance is associated with IgG antibodies to protein S in patients with systemic lupus erythematosus (2009)
  • Author(s): Nojima J, Iwatani Y, Ichihara K, Tsuneoka H, Ishikawa T, Yanagihara M, Takano T, Hidaka Y
  • Journal Title: Thrombosis Research Volume: 124 Pages: 127-131
  • Peer Reviewed
• [Journal Article] 最近注目される微生物-その臨床的意義と検査法、Bartonella quintana, B. hensalae (2009)
  • Author(s): 常岡英弘、柳原正志
  • Journal Title: 臨床と微生物 Volume: 36 Pages: 139-142
• [Journal Article] Acquired activated protein C resistance is associated with IgG antibodies to protein S in patients with systemic lupus erythematosus. (2009)
  • Author(s): Nojima J, et. al.
  • Journal Title: Thrombosis research 124 Pages: 127-131
  • Peer Reviewed
• [Journal Article] 最近注目される微生物-その臨床的意義と検査法Bartonella quintana, Bartonella henselae (2009)
  • Author(s): 常岡英弘, 他
  • Journal Title: 臨床と微生物 36 Pages: 139-142
  • Peer Reviewed
• [Presentation] 感染性心内膜炎患者より分離されたBartonella henselaeの病原因子の遺伝子解析 (2011)
  • Author(s): 菅崎幹樹、柳原正志、常岡英弘
  • Organizer: 第44回中国四国医学検査学会
  • Place of Presentation: アスティとくしま(徳島県)
  • Year and Date: 2011-11-06
• [Presentation] 猫ひっかき病原因菌Bartonella henselaeのIV型分泌装置とBepAの配列多型および病原性との関連性について (2011)
  • Author(s): 柳原正志、常岡英弘
  • Organizer: 第85回日本感染症学会総会
  • Place of Presentation: ザプリンスパークタワー東京(東京都)
  • Year and Date: 2011-04-22
• [Presentation] わが国におけるヒトおよびネコ由来Bartonella henselaeのMultispacer typingによる遺伝子型の分布 (2010)
  • Author(s): 柳原正志、常岡英弘
  • Organizer: 第80回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 松山市総合コミュニティセンター(愛媛県)
  • Year and Date: 2010-11-20
• [Presentation] わが国におけるヒトおよびネコ由来Bartonella henselaeのMultispacer typingによる遺伝子型の分布 (2010)
  • Author(s): 柳原正志
  • Organizer: 第80回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 愛媛県・松山市総合コミュニティセンター
  • Year and Date: 2010-11-20
• [Presentation] 感染性心内膜炎患者より分離されたBartonella henselaeの分子系統解析 (2010)
  • Author(s): 柳原正志、菅崎幹樹、梅田昭子、常岡英弘
  • Organizer: 第63回日本細菌学会中国・四国支部総会
  • Place of Presentation: 松山大学文京キャンパス(愛媛県)
  • Year and Date: 2010-10-17
• [Presentation] 感染性心内膜炎患者より分離されたBartonella henselaeの分子系統解析 (2010)
  • Author(s): 柳原正志
  • Organizer: 第63回日本細菌学会中国・四国支部総会
  • Place of Presentation: 愛媛県・松山大学文京キャンパス
  • Year and Date: 2010-10-17
• [Presentation] Bartonella henselae ST-1型(MLST法)のSpacer配列による亜型分類 (2010)
  • Author(s): 柳原正志、常岡英弘、梅田昭子
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2010-04-06
• [Presentation] Bartonella henselae ST-1型(MLST法)のSpacer配列による亜型分類 (2010)
  • Author(s): 柳原正志
  • Organizer: 第84回日本感染症学会学術集会
  • Place of Presentation: 京都府・国立京都国際会館
  • Year and Date: 2010-04-06
• [Presentation] Presence of 2 different copies of the tRNA-Ala/ GCA-tRNA-Ile/ AUC spacer in Bartonella henselae (2010)
  • Author(s): 柳原正志、常岡英弘、梅田昭子
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-03-29
• [Presentation] Presence of 2 different copies of the tRNA-Ala/GCA-tRNA-Ile/AUC spacer in Bartonella henselae (2010)
  • Author(s): 柳原正志
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川県・パシフィコ横浜
  • Year and Date: 2010-03-29
• [Presentation] 本邦初めてのBartonella henselaeによる感染性心内膜炎-大動脈弁疣贅からの分離例- (2010)
  • Author(s): 常岡英弘、柳原正志、浅利祥子、藤波裕子、長藤宏士
  • Organizer: 第21回日本臨床微生物学会総会
  • Place of Presentation: 東京ドームホテル(東京都)
  • Year and Date: 2010-01-30
• [Presentation] 本邦初めてのBartonella henselaeによる感染性心内膜炎-大動脈弁疣贅からの分離例- (2010)
  • Author(s): 常岡英弘
  • Organizer: 第21回日本臨床微生物学会総会
  • Place of Presentation: 東京都・東京ドームホテル
  • Year and Date: 2010-01-30
• [Presentation] 感染性心内膜炎患者の大動脈弁疣贅からBartonella henselaeを分離した1症例 (2009)
  • Author(s): 浅利祥子、藤波裕子、長藤宏士、柳原正志、常岡英弘
  • Organizer: 第42回中国四国医学検査学会
  • Place of Presentation: サンポートホテル高松(香川県)
  • Year and Date: 2009-10-31
• [Presentation] 感染性心内膜炎患者の大動脈疣贅からBartonella henselaeを分離した1症例 (2009)
  • Author(s): 浅利祥子
  • Organizer: 第42回中国四国医学検査学会
  • Place of Presentation: 香川県・サンポートホール高松
  • Year and Date: 2009-10-31
• [Presentation] MLST法によるわが国のヒトおよびネコ由来Bartonella henselaeの分子系統解析 (2009)
  • Author(s): 柳原正志、常岡英弘、梅田昭子、塚原正人
  • Organizer: 第83回日本感染症学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2009-04-24
• [Presentation] MLST法によるわが国のヒトおよびネコ由来Bartonella henselaeの分子系統解析 (2009)
  • Author(s): 柳原正志
  • Organizer: 第83回日本感染症学会総会
  • Place of Presentation: 東京都・京王プラザホテル
  • Year and Date: 2009-04-24