| Project/Area Number |
21790539
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
KISHIKAWA Naoya Nagasaki University, 大学院・医歯薬学総合研究科, 准教授 (90336181)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | 化学発光 / キノン / 酸化還元 / 標識抗体 / 活性酸素 / ナフトキノン / 酸化還元サイクル / 標識ビオチン / イムノアッセイ / 酵素標識 |
|---|
| Research Abstract |
A strong and long-lived chemiluminescence was observed when quinone was mixed with dithiothreitol (DTT) and luminol, and the chemiluminescence intensity was proportional to the concentration of quinone. Therefore, we considered that quinone-labeled antibody could be applied to develop a non-enzymatic chemiluminescence immunoassay. In this study, quinone-labeled antibody was prepared by coupling of 1,4-dihydro-1,4-dioxo-3-methyl-2-naphthylmethyl chloroformate to amino group of antibody. Since the quinone-labeled antibody emitted chemiluminescence after addition of DTT and luminol, it should become a new analytical tool for various substances based on antigen-antibody interaction.
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