Development of "microRNA-regulating chemoprevention"
| Project/Area Number |
21790555
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hygiene
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TOMOSUGI Mitsuhiro Kyoto Prefectural University of Medicine, 医学部, プロジェクト研究員 (60533429)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 癌 / microRNA / 予防法開発 / 細胞周期停止 / RB / miR-34a / p53 / プロモーター |
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| Research Abstract |
miR-34a is a tumor suppressor microRNA. I searched for the food components that regulated miR-34a expression. As a result from flow cytometry analysis, several kinds of materials inducing G1 arrest were found, using RB inactivated cancer cells.
Real-time PCR specified flavone that induced miR-34a expression from candidate materials. In addition, decrease in target proteins of miR-34a was confirmed by Western blot analysis.
These results suggested that food component could control expression of microRNA in vivo. The research of miR-34a regulated by flavone might realize "microRNA-regulating chemoprevention"
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Report
(3 results)
Research Products
(4 results)
