| Project/Area Number |
21790562
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hygiene
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
TAKATA Ayako St.Marianna University School of Medicine, 医学部, 教授 (30321897)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | アスベスト / ナノ粒子 / メカノケミカル処理 / 中皮腫 / 発がん / 酸化的DNA損傷 |
|---|
| Research Abstract |
The mechanochemical treatment of asbestos is expected to decompose asbestos and transform into new nanomaterials. To evaluate the carcinogenicity of nanomaterials produced by the mechanochemical treatment of chrysotile, we administered chrysotile (CH) and the nanomaterial (CH-M) to rats by a single intraperitoneal injection. The incidence of mesothelioma was determined histopathologically during the 1-year study. In addition, we determined urinary levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker for oxidative DNA damage, and serum levels of N-ERC/mesothelin, a biomarker for mesothelioma. While mesothelioma was detected in 60% of CH-treated rats, it was not observed in rats treated with CH-M. In the CH group, 8-OHdG and N-ERC/mesothelin levels were elevated after day 180. Levels of 8-OHdG and N-ERC/mesothelin were significantly lower in the CH-M group compared to the CH group. Our results collectively suggested that mechanochemical treatment of CH reduced oxidative stress and carcinogenic potential.
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