Development of novel phage antibody libraries for preparation of anti-drug antibodies

Research Project

Project/Area Number	21790610
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Legal medicine
Research Institution	Kumamoto University
Principal Investigator	SASAO Ako Kumamoto University, 大学院・生命科学研究部, 助教 (80284751)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords	社会医学 / 抗薬物抗体 / 法医中毒学 / 組換え抗体
Research Abstract	The aim of this study was to develop phage antibody libraries to facilitate anti-drug antibodies preparation. The hybridoma that produces anti-fluvoxamine (FLV ; antidepressant) antibodies was used for analysis of the epitope sequence. And also, plasmid vector to produce the anti-FLV single chain variable fragment (scFv) was prepared. The hybridoma that produces anti-phenothiazines (antipsychotic) antibodies was also prepared.