The genetic change of pancreatic duct epithelial cells mediated by epithelial mesenchymal transition

• Project/Area Number: 21790645
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Tohoku University
• Principal Investigator: KANNO Atsushi Tohoku University, 病院, 助教 (70509190)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 膵癌 / 慢性膵炎 / K-ras / miRNA / 膵星細胞

Research Abstract

1. The establish of the Human Pancraetic Duct Epithelial Cell and Pancreatic stellate cell with K-ras mutation
We established that Human Pancraetic Duct Epithelial Cell (HPDE) which retroviral vector pBabepuro-KRAS4B^<G12V> was transfected. We confirmed that the HPDE had K-ras mutation. We are trying that retroviral vector pBabepuro-KRAS4B^<G12V> was transfected to the human pancreatic stellate cell and the rat pancreatic stellate cell in the similar medhod.
2. The alteration of the gene by the co-culture of Human Pancraetic Duct Epithelial Cell and Pancreatic stellate cell with K-ras mutation and Pancreatic stellate cell.
(1) We are researching that the alteration of the gene by the co-culture of Human Pancraetic Duct Epithelial Cell and Pancreatic stellate cell with K-ras mutation and Pancreatic stellate cell.
(2) We are examining that the alteration of the miRNA by the co-culture of Human Pancraetic Duct Epithelial Cell and Pancreatic stellate cell with K-ras mutation and Pancreatic stellate cell with miRNA microarray.

Research Products

• [Journal Article] Intraductal papillary mucininous neoplasm of the bile ducts : multimodality assessment with pathologic correlation. (2011)
  • Author(s): Takanami K, Yamada T, Tsuda M, Takase K, Ishida K, Nakamura Y, Kanno A, Shimosegawa T, Unno M, Takahashi S.
  • Journal Title: Abdom Imaging. 印刷中
  • Peer Reviewed
• [Journal Article] 【IPMNにおける「国際診療ガイドライン」の検証】手術例と経過観察例から導き出した分枝型IPMNの治療方針 (2010)
  • Author(s): 菅野敦, 佐藤賢一, 下瀬川徹
  • Journal Title: 胆と膵 30巻3号 Pages: 245-249
• [Journal Article] 類円形を呈した胆管内乳頭状腫瘍の一例 (2010)
  • Author(s): 菅野敦, 佐藤賢一, 廣田衛久, 正宗淳, 片寄友, 海野倫明, 石田和之, 全陽, 下瀬川徹
  • Journal Title: 胆道 23巻4号 Pages: 677-683
  • NAID: 10030360783
  • Peer Reviewed
• [Journal Article] 目でみるトレーニング (2010)
  • Author(s): 菅野敦, 下瀬川徹
  • Journal Title: Medicina 第47巻 第6号 Pages: 1093-1099
• [Journal Article] 術前に診断し得た下部胆管原発腺内分泌細胞癌の1例 (2010)
  • Author(s): 菅野敦, 佐藤賢一, 廣田衛久, 正宗淳, 高舘達之, 力山敏樹, 海野倫明, 石田和之, 下瀬川徹
  • Journal Title: 胆道 24巻5号 Pages: 714-721
  • NAID: 10030361596
  • Peer Reviewed
• [Journal Article] Granular cell tumor of the pancreas : A case report and review of literature. (2010)
  • Author(s): Kanno A, Satoh K, Hirota M, Hamada S, Umino J, Itoh H, Masamune A, Egawa S, Motoi F, Unno M, Ishida K, Shimosegawa T.
  • Journal Title: World J Gastrointest Oncol. 2(2) Pages: 121-124
  • Peer Reviewed
• [Journal Article] Prediction of invasive carcinoma in branch type intraductal papillary mucinous neoplasms of the pancreas. (2010)
  • Author(s): Kanno A, Satoh K, Hirota M, Hamada S,Umino J, Itoh H, Masamune A, Asakura T, Shimosegawa T.
  • Journal Title: J Gastroenterol. 45(9) Pages: 952-959
  • NAID: 10027699047
  • Peer Reviewed
• [Journal Article] Prediction of invasive carcinoma in branch type intraductal papillary mucinous neoplasms of the pancreas. (2010)
  • Author(s): Kanno A, Satoh K, et a
  • Journal Title: J Gastroenterol. Volume: 45 Pages: 952-9
  • NAID: 10027699047
  • Peer Reviewed
• [Journal Article] Intraductal papillary mucininous neoplasm of the bile ducts : multimodality assessment with pathologic correlation. (2010)
  • Author(s): Takanami K, Yamada T, Tsuda M, Takase K, Ishida K, Nakamura Y, Kanno A, et al
  • Journal Title: Abdom Imaging. Volume: 20
  • Peer Reviewed
• [Journal Article] 目でみるトレーニング (2010)
  • Author(s): 菅野敦, 下瀬川徹
  • Journal Title: Medicina Volume: 47 Pages: 1093-1099
• [Journal Article] 術前に診断し得た下部胆管原発腺内分泌細胞癌の1例 (2010)
  • Author(s): 菅野敦, 佐藤賢一, 他
  • Journal Title: 胆道 Volume: 24 Pages: 714-721
  • NAID: 10030361596
  • Peer Reviewed
• [Journal Article] Granular cell tumor of the pancreas : A case report and review of literature (2010)
  • Author(s): Kanno A.Satoh K., et al.
  • Journal Title: World Journal of Gastroenterology 2 Pages: 121-124
  • Peer Reviewed
• [Journal Article] 手術例と経過観察例から導き出した分枝型IPMNの治療方針 (2009)
  • Author(s): 菅野敦, 佐藤賢一, 下瀬川徹
  • Journal Title: 胆と膵 30 Pages: 245-249
• [Journal Article] 類円形を呈した胆管内乳頭状腫瘍の一例 (2009)
  • Author(s): 菅野敦, 佐藤賢一, 他
  • Journal Title: 胆道 23 Pages: 677-683
  • NAID: 10030360783
  • Peer Reviewed
• [Presentation] 自己免疫性膵炎の治療と予後 自己免疫性膵炎の再燃に関する検討 (2010)
  • Author(s): 菅野敦, 佐藤賢一, 下瀬川徹
  • Organizer: 日本消化器病学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-10-13
• [Presentation] Standard steroid therapy for autoimmune pancreatitis (2010)
  • Author(s): A Kanno, T Kamisawa, T Shimosegawa, K Okazaki, T Nishino, H Watanabe, F Okumura, T Nishikawa, K Kobayashi, T Ichiya, H Takatori, K Yamakita, K Kubota, H Hamano, K Okamura, K Hirano, T Ito, S B H Ko, M Omata
  • Organizer: IAP and JPS 2010
  • Place of Presentation: Fukuoka
  • Year and Date: 2010-07-10
• [Presentation] ワークショップ5:自己免疫性膵炎をめぐる新たな展開 IgG4陰性自己免疫性膵炎の診断基準における位づけ (2010)
  • Author(s): 菅野敦, 佐藤賢一, 下瀬川徹
  • Organizer: 第96回日本消化器病学会総会
  • Place of Presentation: 新潟
  • Year and Date: 2010-04-23
• [Presentation] シンポジウム10:膵IPMNの手術適応の見直し分枝型IPMNの手術適応の再検討 (2010)
  • Author(s): 菅野敦, 佐藤賢一, 下瀬川徹
  • Organizer: 第96回日本消化器病学会総会
  • Place of Presentation: 新潟
  • Year and Date: 2010-04-22
• [Presentation] ポスター 膵管内乳頭粘液腫瘍(IPMN)の手術適応と切離線を決定するための検査法 (2009)
  • Author(s): 菅野敦, 佐藤賢一, 下瀬川徹
  • Organizer: JDDW 2009
  • Place of Presentation: 京都
  • Year and Date: 2009-10-17
• [Presentation] ワークショップ 自己免疫性膵炎関連疾患の病因病態IgG4陰性自己免疫性膵炎の臨床的背景の検討 (2009)
  • Author(s): 菅野敦, 佐藤賢一, 下瀬川徹
  • Organizer: JDDW 2009
  • Place of Presentation: 京都
  • Year and Date: 2009-10-14
• [Presentation] 自己免疫性膵炎の治療と予後 自己免疫性膵炎の再燃に関する検討 (2009)
  • Author(s): 菅野敦, 佐藤賢一, 下瀬川徹
  • Organizer: JDDW 2010
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-13
• [Presentation] 下部胆管に発生した腺内分泌細胞癌の一例 (2009)
  • Author(s): 菅野敦, 佐藤賢一, 正宗淳, 廣田衛久, 菊田和弘, 粂潔, 濱田晋, 渡辺崇, 伊藤広通, 下瀬川徹, 高舘達之, 力山敏樹, 海野倫明, 石田和之
  • Organizer: 第45回日本胆道学会
  • Place of Presentation: 千葉
  • Year and Date: 2009-09-18
• [Presentation] ワークショップ2 自己免疫性膵炎診断基準2006の再評価 診断基準の国際化に向けて当科における自己免疫性膵炎診断基準2006の検討 (2009)
  • Author(s): 菅野敦, 佐藤賢一, 下瀬川徹
  • Organizer: 第40回日本膵臓学会大会
  • Place of Presentation: 東京?
  • Year and Date: 2009-07-30
• [Presentation] 膵外病変からみた自己免疫性膵炎 (2009)
  • Author(s): 菅野敦, 佐藤賢一, 下瀬川徹
  • Organizer: 第95回日本消化器病学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-05-07
• [Book] 自己免疫性膵炎 1 超音波見て診て学ぶ 膵腫瘍の画像診断(木村理著) (2010)
  • Author(s): 菅野敦, 佐藤賢一, 下瀬川徹
  • Publisher: 永井書店
• [Book] 見て診て学ぶ 膵腫瘍の画像診断 (2009)
  • Author(s): 菅野敦, 佐藤賢一, 他
  • Total Pages: 391
  • Publisher: 永井書店