Cooperation of TGF-β and Notch in the induction of intestinal metaplasia and cancer stem cells of the esophagus and stomach

• Project/Area Number: 21790647
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Tohoku University
• Principal Investigator: ASANO Naoki Tohoku University, 病院, 助教 (20526454)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 上部消化管学(食道、胃、十二指腸) / ヘリコバクター・ピロリ / TGF-β / 腸上皮化生 / NFκB / NFkB

Research Abstract

Immunohistochemistry of human gastric cancer specimens revealed that only poorly differentiated gastric cancer cells express the stem cell marker CD133. The same tendency was observed in gastric cancer cell lines. We established a CD133 expressing gastric cancer cell line KATOIII that stably express doxycycline inducible Notch1 siRNA vector. Analysis using this and other cell line revealed that CD133 is directly regulated by Notch1 through RBP-Jk. We also found out that Notch1 regulates proliferation and tumorigenesis in these cells.

Research Products

• [Presentation] RBP-J κ dependent Notch1 signaling is required for the maintenance of stem-like cells in diffuse type gastric cancers (2010)
  • Author(s): 浅野直喜、小西秀知、今谷晃、木村修、近藤穣、千葉隆士、宇野要、阿部靖彦、飯島克則、小池智幸、下瀬川徹
  • Organizer: 1st JSGE International Topic Conference
  • Place of Presentation: 鎌倉
  • Year and Date: 2010-09-25
• [Presentation] RBP-Jκ dependent Notch1 signaling is required for the maintenance of stem-like cells in diffuse type gastric cancers (2010)
  • Author(s): 浅野直喜
  • Organizer: The 1st JSGE International Topic Conference
  • Place of Presentation: 鎌倉パークホテル
  • Year and Date: 2010-09-25
• [Presentation] RBP-J κ dependent Notch1 signaling regulates proliferation, tumorigenesis and CD133 in diffuse type gastric cancers (2010)
  • Author(s): 浅野直喜、今谷晃、下瀬川徹
  • Organizer: 第69回日本癌学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-22
• [Presentation] 低分化型胃癌におけるRBP-Jκを介したNotch1シグナルによる増殖、造腫瘍能、CD133発現の制御機構 (2010)
  • Author(s): 浅野直喜
  • Organizer: 第69回日本癌学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-22
• [Presentation] 低分化型胃癌におけるRBP-Jκを介したNotchシグナルによる幹細胞マーカーCD133発現機構 (2009)
  • Author(s): 浅野直喜、小西秀知、今谷晃、近藤穣、下瀬川徹
  • Organizer: 第17回浜名湖シンポジウム
  • Place of Presentation: 浜松
  • Year and Date: 2009-12-22
• [Presentation] ヒト低分化型胃癌におけるNotchシグナルを介した幹細胞マーカーCD133発現と発癌誘導機構 (2009)
  • Author(s): 小西秀知、浅野直喜、今谷晃、近藤穣、小池智幸、飯島克則、下瀬川徹
  • Organizer: JDDW 2009
  • Place of Presentation: 京都
  • Year and Date: 2009-10-15