| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Hepatic stem/progenitor cells (HSPCs) in the liver bud are postulated to migrate into septum transversum mesenchyme at around embryonic day (E) 9 in mice. Mid-fetal livers (E11.5-E14.5) support proliferation and differentiation of hematopoietic stem cells derived from the aorta-gonad-mesonephros region. We established a new co-culture system, using mouse embryonic fibroblast cells, to permit analysis of the phenotypes of early-fetal HSPC candidates. In this co-culture system, CD13^+Dlk^+ cells purified from mouse early-fetal livers (E9.5 and E10.5) formed colonies composed of both albumin-positive hepatocytic cells and cytokeratin (CK) 19-positive cholangiocytic cells, indicating that early-fetal CD13^+Dlk^+ cells have properties of bi-potent progenitor cells. These first prospective studies of early-fetal HSPC candidates demonstrate that bi-potent stem/progenitor cells exist before E11.5.
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