Establishment of intestinal differentiation method from ES/iPS cells
| Project/Area Number |
21790671
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Kumamoto University |
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Principal Investigator |
SHIRAKI Nobuaki Kumamoto University, 発生医学研究所, 助教 (70448520)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ヒトiPS細胞 / ES細胞 / モデル細胞 / 発生分化 / 再生医学 / 細胞・組織 |
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| Research Abstract |
The efficient differentiation of mouse or human ES cells into mature cell types of the intestine remains a major challenge. Using the in vitro differentiation procedure of pluripotent stem cells into definitive endoderm we established previously, we found that simultaneous application of two specific growth factors efficiently induced intestinal differentiation. All four intestinal differentiated cell types, namely the absorptive enterocytes and three types of secretory cells (goblet cells, enteroendocrine cells, Paneth cells) were efficiently differentiated among mouse and human ES cell-derived intestinal epithelium cells.
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Report
(3 results)
Research Products
(79 results)
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[Journal Article] Synthesized basement membrane substrata direct the differentiation of mouse ES cells into pancreatic lineages2010
Author(s)
Higuchi Y., Shiraki N., Yamane K, Qin Z., Mochitate K., Araki K., Senokuchi K., Yamagata K., Hara M., Kume K., Kume S.
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Journal Title
J.Cell Sci. 123
Pages: 2733-2742
Related Report
Peer Reviewed
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[Journal Article] Synthesized basement membrane substrata direct the differentiation of mouse ES cells into pancreatic lineages2010
Author(s)
Higuchi Y., Shiraki N., Yamane K, Qin Z., Mochitate K., Araki K., Senokuchi K., Yamagata K., Hara M., Kume K., Kume S
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Journal Title
J.Cell Sci.
Volume: 123
Pages: 2733-2742
Related Report
Peer Reviewed
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[Journal Article] Differentiation and characterization of embryonic stem cells into three germ layers.2009
Author(s)
Shiraki, N., Higuchi, Y., Harada, S., Umeda, K., Isagawa, T., Aburatani, H., Kume, K., Kume, S.
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Journal Title
Biochem Biophys Res Commun 381
Pages: 694-699
Related Report
Peer Reviewed
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[Presentation] ヒトES細胞から膵前駆細胞への分化誘導2009
Author(s)
Kahoko Umeda., Nobuaki Shiraki., Daisuke Sakano., Kumi Tokieda., Kazuhiko Kume., Shoen Kume.
Organizer
第32回日本分子生物学会
Place of Presentation
パシフィコ横浜(横浜)(ポスター発表)
Related Report
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[Remarks] Higuchi Y., Shiraki N., Yamane K, Qin Z., Mochitate K., Araki K., Senokuchi K., Yamagata K., Hara M., Kume K., Kume S., Synthesized basement membrane substrata direct the differentiation of mouse ES cells into pancreatic lineages, J.Cell Sci., 123, 2733-42, (2010)
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[Remarks] Higuchi Y., Shiraki N., Yamane K., Qin Z., Mochitate K., Araki K., Senokuchi K., Yamagata K., Hara M., Kume K., and Kume S., Synthesized basement membrane substrata direct the differentiation of mouse ES cells into pancreatic lineages, J. Cell Sci., 123, 2733-42, (2010)
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