| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
The efficient differentiation of mouse or human ES cells into mature cell types of the intestine remains a major challenge. Using the in vitro differentiation procedure of pluripotent stem cells into definitive endoderm we established previously, we found that simultaneous application of two specific growth factors efficiently induced intestinal differentiation. All four intestinal differentiated cell types, namely the absorptive enterocytes and three types of secretory cells (goblet cells, enteroendocrine cells, Paneth cells) were efficiently differentiated among mouse and human ES cell-derived intestinal epithelium cells.
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