| Project/Area Number |
21790672
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Kagoshima University |
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Principal Investigator |
KANMURA Shuji Kagoshima University, 医学部・歯学部附属病院, 医員 (60448561)
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | ディフェンシン / 炎症性腸疾患 / 腸管免疫 / 抗菌ペプチド / プロテオミクス / 潰瘍性大腸炎 / サイトカイン / 好中球 / プロイオミクス |
|---|
| Research Abstract |
Human neutrophil peptides (HNPs) 1-3 promoted proliferation of epithelial cell lines at low peptide concentrations ; however, higher peptide concentrations inhibited cellular proliferation. And, treatment with HNPs resulted in the increase expression of interleukin-1 (IL-8), vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1) from epithelial cell lines. Additionally, after administration of HNPs to DSS-induced mouse IBD model, high histological colitis score, weight loss, and shortening of the large intestine were observed as compared to non-treated mice. These results suggest that HNPs increase the expression of proinflammatory cytokines in intestinal epithelial cells and exacerbates experimental colitis.
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