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Epigenomic analysis of gastrointestinal cancer cells

Research Project

Project/Area Number 21790675
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionSapporo Medical University

Principal Investigator

SUZUKI Hiromu  Sapporo Medical University, 医学部, 助教 (20381254)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords消化器癌 / メチル化 / エピゲノム / 癌幹細胞
Research Abstract

In order to dissect the epigenome of gastrointestinal cancer, we analyzed the chromatin signatures and gene expression in colorectal cancer (CRC) cells. We carried out high-throughput ChIP-seq analysis and compared the data with gene expression and microRNA (miRNA) expression signatures. We also examined the DNA methylation and histone modifications in primary CRC tissues. Through these analyses, we identified a number of epigenetically silenced miRNA genes in CRCs. Our study provided a large set of epigenome data in CRC cells, suggesting that the data set are useful tools to identify cancer related genes or miRNAs.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (38 results)

All 2011 2010 2009

All Journal Article (23 results) (of which Peer Reviewed: 23 results) Presentation (12 results) Book (3 results)

  • [Journal Article] Role of DNA Methylation in the Development of Diffuse-Type Gastric Cancer.2011

    • Author(s)
      Yamamoto E, Suzuki H, Takamaru H, Yamamoto H, Toyota M, Shinomura Y.
    • Journal Title

      Digestion 83

      Pages: 241-249

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic inactivation of calcium-sensing receptor in colorectal carcinogenesis.2011

    • Author(s)
      K, Yamamoto H, Taniguchi H, Adachi Y, Nakazawa M, Tanuma T, Kato N, Sukawa Y, Sanchez JV, Suzuki H, Sasaki S, Imai K, Shinomura Y.
    • Journal Title

      Mod Pathol

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Correlation between LINE-1 Hypomethylation and the Malignancy of Gastrointestinal Stromal Tumors.2010

    • Author(s)
      Igarashi S, Suzuki H, Niinuma T, Shimizu H, Nojima M, Iwaki H, Nobuoka T, Nishida T, Miyazaki Y, Takamaru H, Yamamoto E, Yamamoto H, Tokino T, Hasegawa T, Hirata K, Imai K, Toyota M, Shinomura Y.
    • Journal Title

      Clin Cancer Res 16

      Pages: 5114-5123

    • NAID

      130004927691

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Methylation-associated silencing of microRNA-34b/c in gastric cancer and its involvement in an epigenetic field defect.2010

    • Author(s)
      Suzuki H, Yamamoto E, Nojima M, Kai M, Yamano HO, Yoshikawa K, Kimura T, Kudo T, Harada E, Sugai T, Takamaru H, Niinuma T, Maruyama R, Yamamoto H, Tokino T, Imai K, Toyota M, Shinomura Y.
    • Journal Title

      Carcinogenesis 31

      Pages: 2066-2073

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA methylation of interferon regulatory factors in gastric cancer and noncancerous gastric mucosae.2010

    • Author(s)
      Awe Yamashita M, Toyota M, Suzuki H, Nojima M, Yamamoto E, Kamimae S, Watanabe Y, Kai M, Akashi H, Maruyama R, Sasaki Y, Yamano H, Sugai T, Shinomura Y, Imai K, Tokino T, Itoh F.
    • Journal Title

      Cancer Sci 101

      Pages: 1708-1716

    • NAID

      10026594987

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Array-based genomic resequencing of human leukemia.2010

    • Author(s)
      Awe Yamashita Y, Yuan J, Suetake I, Suzuki H, Ishikawa Y, Choi YL, Ueno T, Soda M, Hamada T, Haruta H, Takada S, Miyazaki Y, Kiyoi H, Ito E, Naoe T, Tomonaga M, Toyota M, Tajima S, Iwama A, Mano H.
    • Journal Title

      Oncogene 29

      Pages: 3723-3731

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic dissection of differential signaling threshold requirements for the Wnt/beta-catenin pathway in vivo.2010

    • Author(s)
      MBuchert M, Athineos D, Abud HE, Burke ZD, Faux MC, Samuel MS, Jarnicki AG, Winbanks CE, Newton IP, Meniel VS, Suzuki H, Stacker SA, Nathke IS, Tosh D, Huelsken J, Clarke AR, Heath JK, Sansom OJ, Ernst M.
    • Journal Title

      PLoS Genet 6

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] IGFBP7 is a p53 Responsive Gene Specifically Silenced in Colorectal Cancer with CpG Island Methylator Phenotype.2010

    • Author(s)
      Suzuki H, Igarashi S, Nojima M, Maruyama R, Yamamoto E, Kai M, Akashi H, Watanabe Y, Yamamoto H, Sasaki Y, Itoh F, Imai K, Sugai T, Shen L, Issa JP, Shinomura Y, Tokino T, Toyota M.
    • Journal Title

      Carcinogenesis 31

      Pages: 342-349

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genomic screening for genes upregulated by demethylation revealed novel targets of epigenetic silencing in breast cancer.2010

    • Author(s)
      Fujikane T, Nishikawa N, Toyota M, Suzuki H, Nojima M, Maruyama R, Ashida M, Ohe-Toyota M, Kai M, Nishidate T, Sasaki Y, Ohmura T, Hirata K, Tokino T.
    • Journal Title

      Breast Cancer Res Treat 122

      Pages: 699-710

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular analysis of single isolated glands in gastric cancers and their surrounding gastric intestinal metaplastic mucosa.2010

    • Author(s)
      Sugai T, Habano W, Jiao YF, Toyota M, Suzuki H, Tsukahara M, Koizuka H, Akasaka R, Koeda K, Wakabayashi G, Suzuki K.
    • Journal Title

      Oncol Rep 23

      Pages: 25-33

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Methylation-associated silencing of microRNA-34b/c in gastric cancer and its involvement in an epigenetic field defect.2010

    • Author(s)
      Suzuki H., et al.
    • Journal Title

      Carcinogenesis

      Volume: 31 Pages: 2066-2073

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Correlation between LINE-1 Hypomethylation and the Malignancy of Gastrointestinal Stromal Tumors.2010

    • Author(s)
      Igarashi S., Suzuki H., et al.
    • Journal Title

      Clinical Cancer Research

      Volume: 16 Pages: 5114-5123

    • NAID

      130004927691

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic dissection of differential signaling threshold requirements for the Wnt/beta-catenin pathway in vivo.2010

    • Author(s)
      Buchert M., et al.
    • Journal Title

      PLoS Genetics

      Volume: 6

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic Profiles Distinguish Malignant Pleural Mesothelioma from Lung Adenocarcinoma.2009

    • Author(s)
      Goto Y, Shinjo K, Kondo Y, Shen L, Toyota M, Suzuki H, Gao W, An B, Fujii M, Murakami H, Osada H, Taniguchi T, Usami N, Kondo M, Hasegawa Y, Shimokata K, Matsuo K, Hida T, Fujimoto N, Kishimoto T, Issa JP, Sekido Y.
    • Journal Title

      Cancer Res 69

      Pages: 9073-9082

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genomic screening for genes silenced by DNA methylation revealed an association between RASD1 inactivation and dexamethasone resistance in multiple myeloma.2009

    • Author(s)
      Nojima M, Maruyama R, Yasui H, Suzuki H, Maruyama Y, Tarasawa I, Sasaki Y, Asaoku H, Sakai H, Hayashi T, Mori M, Imai K, Tokino T, Ishida T, Toyota M, Shinomura Y.
    • Journal Title

      Clin Cancer Res 15

      Pages: 4356-4364

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel method, digital genome scanning detects KRAS gene amplification in gastric cancers : involvement of overexpressed wild-type KRAS in downstream signaling and cancer cell growth.2009

    • Author(s)
      Mita H, Toyota M, Aoki F, Akashi H, Maruyama R, Sasaki Y, Suzuki H, Idogawa M, Kashima L, Yanagihara K, Fujita M, Hosokawa M, Kusano M, Sabau SV, Tatsumi H, Imai K, Shinomura Y, Tokino T.
    • Journal Title

      BMC Cancer 9

      Pages: 198-198

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Elevated Dnmt3a activity promotes polyposis in Apc Min mice by relaxing extracellular restraints on Wnt signaling.2009

    • Author(s)
      Samuel MS, Suzuki H, Buchert M, Putoczki TL, Tebbutt NC, Lundgren-May T, Christou A, Inglese M, Toyota M, Heath JK, Ward RL, Waring PM, Ernst M.
    • Journal Title

      Gastroenterology 137

      Pages: 902-913

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Single Recombinant Adenovirus Expressing p53 and p21-targeting Artificial microRNAs Efficiently Induces Apoptosis in Human Cancer Cells.2009

    • Author(s)
      Idogawa M, Sasaki Y, Suzuki H, Mita H, Imai K, Shinomura Y, Tokino T.
    • Journal Title

      Clin Cancer Res 15

      Pages: 3725-3732

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] CHFR, a potential tumor suppressor, downregulates interleukin-8 through the inhibition of NF-kappaB.2009

    • Author(s)
      Kashima L, Toyota M, Mita H, Suzuki H, Idogawa M, Ogi K, Sasaki Y, Tokino T.
    • Journal Title

      Oncogene 28

      Pages: 2643-2653

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic dysregulation of secreted Frizzled-related proteins in multiple myeloma.2009

    • Author(s)
      Jost E, Gezer D, Wilop S, Suzuki H, Herman JG, Osieka R, Galm O.
    • Journal Title

      Cancer Lett 281

      Pages: 24-31

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] IGFBP7 is a p53-responsive gene specifically silenced in colorectal cancer with CpG island methylator phenotype.2009

    • Author(s)
      Suzuki H, et al.
    • Journal Title

      Carcinogenesis 31

      Pages: 342-349

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Elevated Dnmt3a activity promotes polyposis in Apc(Min) mice by relaxing extracellular restraints on Wnt signaling.2009

    • Author(s)
      Samuel MS, et al.
    • Journal Title

      Gastroenterology 137

      Pages: 902-913

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic profiles distinguish malignant pleural mesothelioma from lung adenocarcinoma.2009

    • Author(s)
      Goto Y, et al.
    • Journal Title

      Cancer Research 69

      Pages: 9073-9082

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 大腸癌においてエピジェネティックに不活化したmicroRNAの網羅的解析2010

    • Author(s)
      鈴木拓
    • Organizer
      日本エピジェネティクス研究会
    • Place of Presentation
      米子市
    • Year and Date
      2010-05-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 消化管問質腫瘍におけるHNE-1低メチル化と悪性度との相関2010

    • Author(s)
      山本英一郎, 鈴木拓, 新沼猛, 五十嵐伸一, 山本博幸, 豊田実, 篠村恭久
    • Organizer
      第21回日本消化器癌発生学会総会
    • Place of Presentation
      軽井沢
    • Related Report
      2010 Final Research Report
  • [Presentation] GISTにおけるエピジェネティックな異常とその臨床的意義2010

    • Author(s)
      鈴木拓, 新沼猛, 篠村恭久
    • Organizer
      第18回日本消化器病関連学会週間
    • Related Report
      2010 Final Research Report
  • [Presentation] CIMP大腸癌の起源2010

    • Author(s)
      山本英一郎, 鈴木拓, 山野泰穂, 神前正幸, 甲斐正広, 菅井有, 時野隆至, 今井浩三, 篠村恭久, 豊田実
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] 次世代シークエンサーを用いた遺伝子転写開始点予測とエピゲノム解析への応用2010

    • Author(s)
      泰史, 篠村恭久, 時野隆至, 今井浩三
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] 大腸がん・胃がん細胞におけるDGKGのエピジェネティック制御2010

    • Author(s)
      甲斐正広, 鈴木拓, 山本英一郎, 今井浩三, 豊田実
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] 胃癌におけるmicroRNA34b/cのエピジェネティックな不活化2010

    • Author(s)
      鈴木拓, 山本英一郎, 高丸博之, 五十嵐伸一, 野島正寛, 山本博幸, 豊田実, 今井浩三, 篠村恭久
    • Organizer
      第6回日本消化管学会学術集会
    • Place of Presentation
      福岡
    • Related Report
      2010 Final Research Report
  • [Presentation] 大腸癌においてエピジェネティックに不活性したmicroRNAの網羅的解析2009

    • Author(s)
      鈴木拓
    • Organizer
      第68回日本癌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] 多発胃癌背景胃粘膜における異常メチル化2009

    • Author(s)
      山本英一郎, 豊田実, 鈴木拓, 山本博幸, 山野泰穂, 菅井有, 今井浩三, 篠村恭久
    • Organizer
      第20回日本消化器癌発生学会総会
    • Place of Presentation
      広島
    • Related Report
      2010 Final Research Report
  • [Presentation] 大腸癌におけるIGFBP7のメチル化とCIMPとの相関2009

    • Author(s)
      鈴木拓, 五十嵐伸一, 高丸博之, 新沼猛, 山本英一郎, 野島正寛, 山本博幸, 豊田実, 今井浩三, 篠村恭久
    • Organizer
      第20回日本消化器癌発生学会総会
    • Place of Presentation
      広島
    • Related Report
      2010 Final Research Report
  • [Presentation] 大腸癌においてエピジェネティックに不活化したmicroRNAの網羅的解析2009

    • Author(s)
      鈴木拓, 五十嵐伸一, 高丸博之, 新沼猛, 山本博幸, 時野隆至, 今井浩三, 豊田実, 篠村恭久
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] 大腸発癌におけるDNAメチル化の役割2009

    • Author(s)
      山本英一郎, 山野泰穂, 鈴木拓, 野島正寛, 丸山玲緒, 時野隆至, 今井浩三, 篠村恭久, 豊田実
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Book] バイサルファイトPCR法.(改訂第5版新遺伝子工学ハンドブック)(村松正實, 山本雅, 岡崎康司編)2010

    • Author(s)
      鈴木拓, 豊田実
    • Publisher
      東京 羊土社
    • Related Report
      2010 Final Research Report
  • [Book] 改訂第5版 新遺伝子工学ハンドブック,村松正實,山本雅,岡崎康司編2010

    • Author(s)
      鈴木拓
    • Publisher
      羊土社
    • Related Report
      2010 Annual Research Report
  • [Book] Inflammation and Cancer Methods and Protocols : Volume 2: Molecular Analysis and Pathways (Inflammation-related aberrant patterns of DNA methylation : detection and role in epigenetic deregulation of cancer cell transcriptome.)(Methods in Molecular Biology Edited by Serguei V.Kozlov.)2009

    • Author(s)
      Suzuki H, Toyota M, Kondo Y, Shinomura Y.
    • Total Pages
      512
    • Publisher
      Humana Press, New York, USA.
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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