Analysis for mechanism of ventricular and atrial cardiomyocyte differentiation in pluripotent stem cells

Research Project

Project/Area Number	21790746
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Circulatory organs internal medicine
Research Institution	Keio University
Principal Investigator	TANAKA Tomofumi Keio University, 医学部, 研究員 (20468482)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	多能性幹細胞 / ES細胞 / iPS細胞 / 分化誘導 / 心筋細胞 / 再生医学 / ips細胞 / 心室筋 / 心房筋
Research Abstract	It has been appreciated that ES/iPS cells can differentiate into heterogeneous populations of atrial, ventricular, and specialized conduction cardiomyocytes in culture. We found that transient inhibition of BMP signaling by Noggin and ascorbic acid treatment induced atrial cardiomyocyte differentiation of mouse ES and human iPS cells. Furthermore, we found that G-CSF treatment enhanced proliferations of human iPS cell-derived cardiomyocytes.