| Project/Area Number |
21790758
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
NAGAI Katsura Hokkaido University, 大学院・医学研究科, 助教 (40374293)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 非閉塞性肺疾患癌 / 肺線維症 / 呼吸器感染症 / 加齢 / 喫煙 / 閉塞性肺疾患 / グルタチオン化 |
|---|
| Research Abstract |
1) Protein S-glutathionylation in the epithelial lining fluid during allergic airway inflammation. The S-glutathionylated protein in bronchoalveolar fluid (BALF) was temporally present only at 8 h in ovalbumin(OVA) stimulated eosinophilic inflammation model. The level of glutaredoxin1 (GRx1) was significantly higher at 24 and 48 h. 2) Aging affects the airway expression of NF-E2-Related Factor 2 (Nrf2) and γ-glutamyl cysteine synthetase regulatory subunit (GCLm) in response to cigarette smoke. Airway epithelial cells from the subsegmental bronchus were harvested by brushing through a flexible bronchoscope (n=30). The levels of Nrf2 mRNA and GCLm mRNA were significantly decreased in current smokers compared to never-smokers in the old subjects, but not in the young subjects. The ratio of nuclear Nrf2-positive cells tended to be increased (p=0.008) in current smokers compared to never-smokers in the young subjects, but not in old subjects.
|
