Isolation and characterization of a human monoclonal antibody against M2 of influenza A virus for reduction of avian influenza
| Project/Area Number |
21790767
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | University of Toyama |
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Principal Investigator |
OZAWA Tatsuhiko University of Toyama, 大学院・医学薬学研究部(医学), 助教 (10432105)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | インフルエンザ / M2 / 抗体医薬 / インフルエザ |
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| Research Abstract |
In this study, we applied ISAAC method to detect influenza virus-specific B-cells in peripheral blood from healthy volunteers who had been immunized with influenza HA vaccine to generate human influenza monoclonal antibodies. We obtained many human monoclonal antibodies reacting influenza viruses, and characterized their functions. One of them (Ab1-10) specifically bound external domain of the matrix protein 2 (M2e). We demonstrate that Ab1-10 reacted not only to seasonal influenza A viruses, but also to pandemic (H1N1) 2009 virus (2009 H1N1) and highly pathogenic avian influenza A virus. More importantly, it inhibited 2009 H1N1 viral replication in vitro, suggesting that it could be a potent candidate for medical application.
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Report
(3 results)
Research Products
(49 results)
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[Presentation] A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood.2009
Author(s)
Ozawa T, Jin A, Tajiri K, Obata T, Kondo S, Kinoshita K, Kadowaki S, Takahashi K, Sugiyama T, Kishi H, Muraguchi A.
Organizer
第32回日本分子生物学会年会
Place of Presentation
横浜
Related Report
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