Development of inhaled biodegradable nano/micro-particles incorporated immuno-modulatory DNA for treatment of inflammatory lung diseases

• Project/Area Number: 21790778
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Yokohama City University
• Principal Investigator: SATO Takashi Yokohama City University, 医学部, 助教 (70510436)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 免疫調節性DNA / ナノ粒子

Research Abstract

Recently we have developed biomaterials incorporated oligodeoxynucleotide (ODN) achieving acceleration of epithelial cell repair. In this regard, we focused on the effective delivery of ODN to pulmonary immune cells such as alveolar macrophages and bronchial associated lymphoid tissue. Finally, we synthesized biodegradable polyketal nano-particles containing ODN, which showed sustaining-hydrolyzed and preferably degraded in acidic condition. Thereby, we could extend our studies in the local administrative inhaled approach in lung inflammation study.

Research Products

• [Journal Article] The acceleration of wound healing in primates by the local administration of immunostimulatory CpG oligonucleotides. (2011)
  • Author(s): Yamamoto M, Sato T, Baren J, Verthelyi D, Klinman DM
  • Journal Title: Biomaterials. 32 Pages: 4238-4242
• [Journal Article] The acceleration of wound healing in primates by the local administration of immunostimulatory CpG oligonucleotides. (2011)
  • Author(s): Yamamoto M, Sato T, et al.
  • Journal Title: Biomaterials Volume: 32(掲載確定) Pages: 4238-42
  • Peer Reviewed
• [Journal Article] Accelerated wound healing mediated by activation of Toll-like receptor 9. (2010)
  • Author(s): Sato T, Yamamoto M, Shimosato T, Klinman DM
  • Journal Title: Wound Repair Regen. 18 Pages: 586-593
  • NAID: 10031181701
• [Journal Article] CpG oligodeoxynucleotides induce strong up-regulation of interleukin 33 via Toll-like receptor 9. (2010)
  • Author(s): Shimosato T, Fujimoto M, Tohno M, Sato T, Tateo M, Otani H, Kitazawa H
  • Journal Title: Biochem Biophys Res Commun. 394 Pages: 81-86
  • NAID: 120007106179
• [Journal Article] Accelerated wound healing mediated by activation of Toll-like receptor 9 (2010)
  • Author(s): Sato T, Yamamoto M, et al.
  • Journal Title: Wound Repair and Regeneration Volume: 18 Pages: 586-593
  • NAID: 10031181701
  • Peer Reviewed
• [Journal Article] CpG oligodeoxynucleotides induce strong up-regulation of interleukin 33 via Toll-like receptor 9. (2010)
  • Author(s): Shimosato T, Sato T, et al.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 394 Pages: 81-86
  • NAID: 120007106179
  • Peer Reviewed
• [Journal Article] Therapeutic application and mechanisms underlying the activity of immunosuppressive oligonucleotides. (2009)
  • Author(s): Klinman DM, Tross D, Klaschik S, Shirota H, Sato T
  • Journal Title: Ann NY Acad Sci. 1175 Pages: 80-88
• [Journal Article] Identification of a potent immunostimulatory oligodeoxynucleotide from the Streptococcus thermophilus lacZ. (2009)
  • Author(s): Shimosato T, Tohno M, Sato T, Kitazawa H.
  • Journal Title: Anim Sci J. 80 Pages: 597-604
  • NAID: 10027887689
• [Journal Article] CpG oligonucleotides as adjuvants for vaccines targeting infectious diseases. (2009)
  • Author(s): Klinman DM, Klaschik S, Sato T, Tross D
  • Journal Title: Adv Drug Deliv Rev. 61 Pages: 248-255
• [Journal Article] CpG oligonucleotied as adjuvants for vaccines targeting infectious diseases (2009)
  • Author(s): Klinman DM, Klaschik S, Sato T, et al.
  • Journal Title: Advaced Drug Delivery Reviews 61 Pages: 248-255
  • Peer Reviewed
• [Journal Article] Therapeutic Application and Mechanisms Underlyinng the Activity of Immunosupp ressive Oligonucleotides (2009)
  • Author(s): Klinman DM. Tross D, Klaschik S, Shirota H, Sato T
  • Journal Title: Ann NY Acad Sci 1175 Pages: 80-88
  • Peer Reviewed
• [Journal Article] CpG oligodeoxynucleotide incorporated basement membrane extract facilitate wound repair in mice (2009)
  • Author(s): Sato T, Shimosato T, Klinman DM.
  • Journal Title: J Immunol (Abstract) 182 Pages: 135-145
  • Peer Reviewed
• [Presentation] CPG Oligodeoxynucleotides Promote Wound Healing In Airway Epithelial Cells. (2011)
  • Author(s): Sato T, Miyazawa N, Klinman DM, Shimosato T, Yamamoto M, Ishigatsubo Y
  • Organizer: Am J Respir Crit Care Med 183:A2087
• [Presentation] Accelerated wound healing in mice by local administration of sustained-release CpG oligodeoxynucleotide (2010)
  • Author(s): Sato Takashi
  • Organizer: 97^<th> American Association of Immunologist, Annual Meeting (Immunology 2010)
  • Place of Presentation: ボルチモア・米国
  • Year and Date: 2010-05-10
• [Presentation] Accelerated wound healing in mice by local administration of sustained-release CpG oligodeoxynucleotide. (2010)
  • Author(s): Sato T, Shimosato T, Yamamoto M, Kobayashi N, Miyazawa N, Klinman DM
  • Organizer: 97th American Association of Immunologist Annual Meeting 2010 J Immunol 184:136.4
• [Presentation] CpG oligodeoxynucleotide incorporated basement membrane extract facilitate wound repair in mice (2009)
  • Author(s): Sato T, Shimosato T, Klinman DM.
  • Organizer: Immunology 2009(第96回米国免疫学会年次集会)
  • Place of Presentation: シアトル・米国
  • Year and Date: 2009-05-13
• [Presentation] CpG oligodeoxynucleotide incorporated basement membrane extract facilitate wound repair in mice. (2009)
  • Author(s): Sato T, Shimosato T, Klinman DM
  • Organizer: 96th American Association of Immunologist Annual Meeting 2009 J Immunol 182:135.5
• [Presentation] CpG oligodeoxynucleotide from Streptococcus thermophilus regulates anti-inflammatory responses (2009)
  • Author(s): Shimosato T, Tohno M, Sato T, Kitazawa H
  • Organizer: ADSA-CSAS-ASAS2009 Joint Annual Meeting
• [Presentation] CpG oligodeoxynucleotide from Streptococcus thermophilus lacZ strongly induces the expression of interleukin-33 in murine immune cells (2009)
  • Author(s): Shimosato T, Tohno M, Sato T, Tateo M, Otani H, Kitazawa H
  • Organizer: The 9th World Congress on Inflammation, The 9th World Congress on Inflammation Book of Abstracts 58:120-121
• [Remarks] ホームページ等
  • URL: http://www.yokohama.ac.jp
• [Remarks]
  • URL: http://www.yokohama.ac.jp
• [Remarks]
  • URL: http://www.yokohama.ac.jp
• [Patent(Industrial Property Rights)] USE OF CPG OLIGODEOXYNUCLEOTIDES TO INDUCE EPITHELIAL CELL GROWTH (2009)
  • Inventor(s): Sato Takashi, Klinman Dennis
  • Industrial Property Rights Holder: Sato Takashi, Klinman Dennis
  • Filing Date: 2009-04-16
• [Patent(Industrial Property Rights)] USE OF CpG OLIGODEOXYNUCLE OTIDES TO INDUCE EPITHELIAL CELL GROWTH (2009)
  • Inventor(s): Sato T, Klinman DM
  • Industrial Property Rights Holder: Sato T, Klinman DM
  • Filing Date: 2009-04-29
  • Overseas
• [Patent(Industrial Property Rights)] METHOD OF TREATING PNEUMOCONIOSIS WITH OLIGODEOX YNUCLEOTIDES (2009)
  • Inventor(s): Sato T, Klinman DM
  • Industrial Property Rights Holder: Sato T, Klinman DM
  • Acquisition Date: 2009-11-26
  • Overseas