Study for COPD and its comorbidity using new COPD animal model

• Project/Area Number: 21790790
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Kurume University
• Principal Investigator: MATSUNAGA Kazuko Kurume University, 医学部, 助教 (60333233)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: COPD / 動物モデル / chitinase / キチナーゼ / マウスモデル / 併存症 / 炎症

Research Abstract

In this study, we analyzed the lung mRNA expression profiles of a murine model of COPD developed using a lung-specific IL-18-transgenic mouse. In this transgenic mouse, the expression of 608 genes was found to vary more than 2-fold in comparison with control WT mice, and was clustered into 4 groups. The expression of 140 genes was constitutively increased at all ages, 215 genes increased gradually with aging, 171 genes decreased gradually with aging, and 82 genes decreased temporarily at 9 weeks of age. Interestingly, the levels of mRNA for the chitinase-related genes chitinase 3-like 1 (Chi3l1), Chi3l3, and acidic mammalian chitinase (AMCase) were significantly higher in the lungs of transgenic mice than in control mice. The level of Chi3l1 protein increased significantly with aging in the lungs of IL-18 transgenic, but not WT mice. Thus our results suggest that chitinase-related genes may play an important role in establishing pulmonary inflammation and emphysematous changes in smokers and COPD patients.

Research Products

• [Journal Article] Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis. (2011)
  • Author(s): Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka SI, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H.
  • Journal Title: Arthritis Res Ther 13:R52.
  • Peer Reviewed
• [Journal Article] Elevated levels of thioredoxin 1 in the lungs and sera of idiopathic pulmonary fibrosis, non-specific interstitial pneumonia and cryptogenic organizing pneumonia (2010)
  • Author(s): Iwata Y, Okamoto M, Hoshino T, Kitasato Y, Sakazaki Y, Tajiri M, Matsunaga K, Azuma K, Kawayama T, Kinoshita T, Imaoka H, Fujimoto K, Kato S, Yano H, Aizawa H.
  • Journal Title: Intern Med 49 Pages: 2393-2400
  • NAID: 130000413366
  • Peer Reviewed
• [Journal Article] Elevated levels of thioredoxin 1 in the lungs and sera of idiopathic pulmonary fibrosis, non-specific interstitial pneumonia and cryptogenic organizing pneumonia. (2010)
  • Author(s): Iwata Y, Matsunaga K, 他
  • Journal Title: Intern Med Volume: 49 Pages: 2393-2400
  • NAID: 130000413366
  • Peer Reviewed
• [Journal Article] Streptomyces pneumoniaの1例 (2009)
  • Author(s): 松永和子, 永田忍彦, 若松謙太郎, 岩田安弘, 熊副洋幸, 古森雅志, 赤崎卓, 加治木章, 北原義也, 原田実根, 相澤久道
  • Journal Title: 日本呼吸器学会雑誌 47 Pages: 569-574
  • NAID: 10027676475
  • Peer Reviewed
• [Journal Article] 自家蛍光電子気管支内視鏡検査が高度扁平上皮異形成の経過観察に有用であった1例 (2009)
  • Author(s): 若松謙太郎, 永田忍彦, 古森雅志, 麻生達磨, 小野聡子, 田口和仁, 松永和子, 赤崎卓, 熊副洋幸, 加治木章, 北原義也, 原田実根
  • Journal Title: 日本胸部臨床 68 Pages: 985-990
  • Peer Reviewed
• [Journal Article] 右傍気管嚢胞の1例. (2009)
  • Author(s): 熊副洋幸, 加治木章, 永田忍彦, 麻生達磨, 小野聡子, 赤崎卓, 田口和仁, 松永和子, 古森雅志, 若松謙太郎, 北原義也, 原田実根, 中園貴彦, 工藤祥.
  • Journal Title: 日本呼吸器学会雑誌 47 Pages: 180-183
  • NAID: 10024823696
  • Peer Reviewed
• [Presentation] Effects of fluticasone and ciclesonide on pulmonary function and airway inflammation in stable mild asthmatics (2009)
  • Author(s): K.Matsunaga, 他
  • Organizer: European Respiratory Society Annual Congress 2009
  • Place of Presentation: Vienna, Austria
• [Remarks] ホームページ等
• [Remarks]
  • URL: http://www.med.kurume-u.ac.jp/med/imed1/index.html
• [Remarks]
  • URL: http://www.med.kurume-u.ac.jp/med/imedl/index.html