Establishment of assay system of renal generaration and development of livestock animal model for parmanent production of donor kidney

• Project/Area Number: 21790794
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: University of Tsukuba
• Principal Investigator: USUI Joichi 筑波大学, 医学医療系, 講師 (70447340)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 腎臓学 / 再生医学 / 幹細胞 / 腎移植 / 腎臓発生 / 糸球体腎炎

Research Abstract

I have progressed to develop how to produce donor kidneys using kidney stem cells and kidney developmental mechanism. I made studies of the production of donor kidney with iPS/ES cells, the establishment of in vitro assay for kidney differentiation, expansion to human kidney diseases and livestock animal experiment.

Research Products

• [Journal Article] Generation of kidney from pluripotent stem cells via blastocyst complementation. (2012)
  • Author(s): Usui J, et al.
  • Journal Title: Am J Pathol Volume: 180 Issue: 6 Pages: 2417-2426
  • DOI: 10.1016/j.ajpath.2012.03.007
  • Peer Reviewed
• [Journal Article] 腎疾患アプローチのための解剖生理 (2012)
  • Author(s): 臼井丈一、山縣邦弘
  • Journal Title: 薬局 Volume: Vol.63 Pages: 972-975
• [Journal Article] 腎疾患アプローチのための解剖生理 (2012)
  • Author(s): 臼井丈一
  • Journal Title: 薬局 Volume: 63 Pages: 972-975
• [Journal Article] Generation of kidney from pluripotent stem cells via blastocyst complementation (2012)
  • Author(s): Usui J, et al.
  • Journal Title: Am J Pathol Volume: (In press)
  • Peer Reviewed
• [Journal Article] 研究の窓辺 (2011)
  • Author(s): 臼井丈一
  • Journal Title: 血圧 Volume: Vol.18 Pages: 1272-1273
• [Journal Article] 腎疾患アプローチのための解剖生理 (2011)
  • Author(s): 臼井丈一、山縣邦弘
  • Journal Title: 薬局 Volume: Vol.62 Pages: 538-541
• [Journal Article] 腎疾患アプローチのための解剖生理 (2011)
  • Author(s): 臼井丈一, 他
  • Journal Title: 薬局 Volume: 62 Pages: 538-541
• [Journal Article] Generation of rat pancreas in mouse by interspecific blastocyst injection of pluripotent stem cells. (2010)
  • Author(s): Kobayashi T,...Usui J et al.
  • Journal Title: Cell Volume: 142 Pages: 787-799
  • Peer Reviewed
• [Journal Article] 腎疾患アプローチのための解剖生理 (2010)
  • Author(s): 臼井丈一、山縣邦弘
  • Journal Title: 薬局 Volume: Vol.61 Pages: 500-503
• [Journal Article] Generation of rat pancreas in mouse by interspecific blastocyst injection of pluripotent stem cells (2010)
  • Author(s): Kobayashi T, et al.
  • Journal Title: Cell Volume: 142 Pages: 787-799
  • Peer Reviewed
• [Journal Article] 腎疾患アプローチのための解剖生理 (2010)
  • Author(s): 臼井丈一, 他
  • Journal Title: 薬局 61 Pages: 500-503
• [Presentation] 遺伝子改変マウスを用いた腎臓再構築の試み (2013)
  • Author(s): 西中村隆一、...臼井丈一
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜、横浜市
  • Year and Date: 2013-03-22
• [Presentation] 腎臓欠損マウスに作成したiPS細胞キメラ腎臓(腎臓再生モデル)における脈管形成の解析 (2012)
  • Author(s): 臼井丈一
  • Organizer: 第55回日本腎臓学会学術総会
  • Place of Presentation: パシフィコ横浜、横浜市
  • Year and Date: 2012-06-01
• [Presentation] 腎臓欠損モデルマウスとblastocyst complementation を組み合わせたマウスES/iPS細胞由来腎臓の作成 (2009)
  • Author(s): 臼井丈一
  • Organizer: 第15回分子腎臓研究会
  • Place of Presentation: 京都市
  • Year and Date: 2009-09-05
• [Presentation] 腎臓欠損モデルマウスと blastocyst complementation を組み合わせたマウスES/iPS細胞由来腎臓の作成 (2009)
  • Author(s): 臼井丈一, 他
  • Organizer: 第15回分子腎臓研究会
  • Place of Presentation: 京都
  • Year and Date: 2009-09-05
• [Presentation] 腎臓欠損マウスに作成したiPS細胞キメラ腎臓（腎臓再生モデル）における脈管形成の解析
  • Author(s): 臼井丈一
  • Organizer: 第55回日本腎臓学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Remarks] ホームページ等筑波大学腎臓内科学研究紹介HP
  • URL: http://www.tsukuba-igaku-kidney.com/up/index.php?mode=s&cate=3&seq=40
• [Remarks] 筑波大学臨床医学系腎臓内科研究一覧
  • URL: http://www.tsukuba-igaku-kidney.com/up/index.php?mode=s&cate=3&seq=40
• [Remarks]
  • URL: http://www.tsukuba-igaku-kidney.com/up/index.php?mode=s&cate=3&seq=40
• [Remarks]
  • URL: http://www.tsukuba-igaku-kidney.com/up/index.php?mode=s&cate=3&seq=40
• [Patent(Industrial Property Rights)] BLASTOCYST COMPLEMENTATIONを利用した臓器再生法 (2008)
  • Inventor(s): 中内啓光,小林俊寛,李允秀,臼井丈一
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Filing Date: 2008-08-28
  • Overseas