Assessment of the possibility for treating renal anemia by targeting SHP-1.

• Project/Area Number: 21790811
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Okayama University
• Principal Investigator: AKAGI Shigeru Okayama University, 大学院・医歯薬学総合研究科, 助教 (80509315)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: SHP-1 / 腎性貧血 / 遺伝子導入 / 骨髄造血前駆細胞

Research Abstract

To determine whether SHP-1 is a therapeutic target molecule for renal anemia, SHP-1 siRNA induction was performed to knockdown SHP-1 expression in renal anemia model mice. The c-kit positive hematopoietic progenitor cells were isolated from bone marrow of 5/6 nephrectomized mice, and transfection of the c-kit positive hematopoietic progenitor cells with SHP-1 siRNA and shRNA was attempted. Eventually, the experiment was not successful maybe due to the condition for transfection or cell viability.