Therapeutic approach for kidney diseases by regenerative medicine

• Project/Area Number: 21790816
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Kochi University
• Principal Investigator: INOUE Kosuke 高知大学, 教育研究部医療学系, 助教 (00403907)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 腎臓 / 再生医学 / 急性腎不全 / 尿細管障害 / 急性腎障害 / dedifferentiation / 再生医療 / 尿細管 / オートファジー / アポトーシス / 血液透析 / 腎保護作用

Research Abstract

The patients of ESKD（End Stage of Kidney Disease） increase up-to 300,000 in Japan. However the therapeutic methods are still limited. We tried novel regenerative methodology to develop new therapies to kidney diseases. Our result shows that Delta/Notch/Hes pathway is activated after ischemic acute kidney injury and plays a role in the proliferation of renal tubules. To regulate embryonic genes could provide a renewable source of cells for use in regenerative therapy for renal diseases.

Research Products

• [Journal Article] Aldosterone Stimulates nuclear factor-kappa B activity and transcription of intercellular adhesion molecule-1 and connective tissue growth factor in rat mesangial cells via serum-and glucocorticoid-inducible protein kinase-1 (2012)
  • Author(s): Terada Y, Ueda S, Hamada ,Shimamura Y, Ogata K, Inoue K, Taniguchi Y, Kagawa T, Horino T, Takao T
  • Journal Title: Clin Exp Nephrol(2012) Volume: 16 Pages: 81-88
  • NAID: 10030342885
  • Peer Reviewed
• [Journal Article] Upregulation of HNF1 during experimental acute kidney injury palys a crucial role in renal tuble regeneration (2012)
  • Author(s): Ogata K, Shimamura Y, Hamada K,Hisa M, Bun M,Okada N, Inoue K, Taniguchi Y, Ishinara M, Kagawa T, Horino T, Fujimoto S, Terada Y
  • Journal Title: Am J Physiol Reneal Physiol
  • Peer Reviewed
• [Journal Article] Serum levels of soluble secreted α-Klotho are decmaking it a probable novel biomarker for early diagnosisreased in the early stages of chronic kindney disease (2012)
  • Author(s): Shimamura Y, Hamada K, Inoue K, Ogata K, Ishihara M, Kagawa T, Inoue M, Fujimoto S, Ikebe M, Yuasa K, Yamanaka S, Sugiura T, Terada Y
  • Journal Title: Clin Exp Nephrol Volume: 16 Pages: 722-729
  • Peer Reviewed
• [Journal Article] Upregulation of HNF-1β during experimental acute kidney injury plays a crucial role in renal tubule regeneration. (2012)
  • Author(s): Ogata K,Inoue K,et al
  • Journal Title: 10.1152/ajprenal.00086 Volume: 303 Issue: 5 Pages: 689-699
  • DOI: 10.1152/ajprenal.00086.2012
  • Peer Reviewed
• [Journal Article] Aldosterone stimulates nuclear factor-kappa B activity and transcription of intercellular adhesion molecule-1 and connective tissue growth factor in rat mesangial cells via serum- and glucocorticoid-inducible protein kinase-1 (2012)
  • Author(s): Terada Y, Inoue K, et al
  • Journal Title: Clin Exp Nephrol Volume: 16 Pages: 81-88
  • NAID: 10030342885
  • Peer Reviewed
• [Journal Article] Possible involvement of intracellular angiotensin IIreceptor in high-glucose-induceddamage in renal proximal tubular cells (2011)
  • Author(s): Takao T, Horino T, Kagawa T, Matsumoto R, Shimamura Y,Ogata K, Inoue K, Taniguchi Y, Taguchi T, Morita T, Terada Y
  • Journal Title: Journal of Nephrology Volume: 24(3) Pages: 218-224
  • Peer Reviewed
• [Journal Article] 急性腎障害における尿中バイオマーカーの現状 (2011)
  • Author(s): 井上紘輔,緒方巧二,島村芳子,香川 亨,寺田典生
  • Journal Title: 腎と透析 Volume: vol.70(3) Pages: 339-341
• [Journal Article] AKIの新規治療法の開発 (2011)
  • Author(s): 井上紘輔,島村芳子,緒方巧二,石原正行,香川 亨,寺田典生
  • Journal Title: 腎臓 Volume: vo1.34(2) Pages: 103-106
• [Journal Article] Possible involvement of intracellular angiotensin II receptor in high-glucose-induced damage in renal proximal tubular cells (2011)
  • Author(s): Takao T, Terada T, et al
  • Journal Title: Journal of Nephrology Volume: 24 Issue: 2 Pages: 218-224
  • DOI: 10.5301/jn.2010.5785
  • Peer Reviewed
• [Journal Article] Possible involvement of intracellular angiotensin II receptor in high-glucose-induced damage in renal proximal tubular cells. (2011)
  • Author(s): Takao T,, Inoue K, Taniguchi Y, Taguchi T, Morita T, Terada Y.
  • Journal Title: Journal of Nephrology. Volume: 24 Pages: 218-224
  • Peer Reviewed
• [Journal Article] Cisplatin-induced macroautophagy occurs prior to apoptosis in proximal tubules in vivo (2010)
  • Author(s): Inoue K, Kuwana H, Shimamura Y, Ogata K, Taniguchi Y, Kagawa T, Horino T, Takao T, Morita T, Sasaki S, Mizushima N, Terada Y
  • Journal Title: Clin Exp Nephrol Volume: 14 Pages: 112-122
  • NAID: 10027228406
  • Peer Reviewed
• [Journal Article] 急性腎不全と慢性腎不全の鑑別 (2010)
  • Author(s): 井上紘輔,寺田典生
  • Journal Title: 総合臨床 Volume: 第59巻第6号 Pages: 1350-1353
• [Journal Article] 薬物によるCKD.腎と透析vol69増刊号 (2010)
  • Author(s): 井上紘輔,緒方巧二,島村芳子,寺田典生
  • Journal Title: AKIとCKDのすべて Volume: vol69 Pages: 201-203
• [Journal Article] Cisplatin-induced macroautophagy occurs prior to apoptosis in proximal tubules in vivo (2010)
  • Author(s): Inoue K, Terada Y, et al
  • Journal Title: Clinical and Experimental Nephrology Volume: 14 Pages: 112-122
  • NAID: 10027228406
  • Peer Reviewed
• [Journal Article] Mechanism of renal tubular regeneration in acute kidney injury. (2010)
  • Author(s): Terada Y, Inoue K.
  • Journal Title: Nippon Jinzo Gakkai Shi. Volume: 52 Pages: 558-561
  • NAID: 10026607962
• [Journal Article] Cisplatin-induced macroautophagy occurs prior to apoptosis in proximal tubules in vivo (2010)
  • Author(s): Inoue K, Terada Y, et al
  • Journal Title: Clinical and Experimental Nephrology 14 Pages: 112-122
  • NAID: 10027228406
  • Peer Reviewed
• [Presentation] 腎臓病におけるオートファジーの意義 (2012)
  • Author(s): 井上紘輔,香川 亨,寺田典生
  • Organizer: 第55回 日本腎臓学会学術総会ワークショップ
  • Place of Presentation: 横浜
  • Year and Date: 2012-06-03
• [Presentation] Small Heat Shock Protein beta-1 (HSPB1 or HSP27) Is Up-Regulated and Regulates Autophagy and Apoptosis of Renal Tubular Cells in the Acute Kidney Injury In Vitro and In Vivo (2012)
  • Author(s): Inoue K, Ogata K, Ishihara M, Urushido M, Okada N, Shimamura Y, Hamada K, Kagawa T, Takao T, Terada Y
  • Organizer: KIDNEY WEEK 2012
  • Place of Presentation: San Diego,USA
• [Presentation] Small heat shock protein beta-1 (HSPB1 or HSP27) is up-regulated and regulates autophagy and apoptosis of renal tubular cells in the acute kidney injury in vitro and in vivo (2012)
  • Author(s): Inoue K
  • Organizer: アメリカ腎臓病学会
  • Place of Presentation: サンディエゴコンベンションセンター（USA)
• [Presentation] Urinary IL-18 is the most useful early predictive biomarker of contrast-induced nephropathy (CIN) on chronic kidney disease (CKD) stage 3 patients in comparison with NGAL and L-FABP (2011)
  • Author(s): Inoue K, Terada Y
  • Organizer: American Society of Nephrology 44th Annual Meeting and Scientific Exposition
  • Place of Presentation: ペンシルバニアコンベンションセンター(米国)
  • Year and Date: 2011-11-11
• [Presentation] Urinary IL-18 and L-FABP as early predictive biomarkers in contrast-in duced AKI(acute kidney injury)on Chronic kidney disease (CKD)stage 3 patients. (2010)
  • Author(s): Inoue K, Terada Y
  • Organizer: American Society of Nephrology 43th Annual Meeting and Scientific Exposition
  • Place of Presentation: 米国コロラド州デンバーコンベンションセンター
  • Year and Date: 2010-11-20
• [Presentation] Cisplatin-Induced Macroautophagy Occurs Prior to Apoptosis in Proximal Tubules In Vivo. (2009)
  • Author(s): Kosuke I., et al
  • Organizer: The American society. of Nephrology Renal week
  • Place of Presentation: 米国カリフォルニア州サンディエゴ
  • Year and Date: 2009-10-30
• [Presentation] Cisplatin-Induced Macroautophagy Occurs Prior to Apoptosis in Proximal Tubules In Vivo (2009)
  • Author(s): Inoue K, Kuwana H, Shimamura Y, Ogata , Taniguchi Y, Kagawa T, Horino T, Takao T, Morita T, Ikeda Y, Sasaki S, and Terada Y
  • Organizer: The American society of NephrologyRenal week
  • Place of Presentation: San Diego,USA
• [Book] AKIの管理Q＆A (2012)
  • Author(s): 井上 紘輔
  • Total Pages: 6
  • Publisher: 総合医学社
• [Book] 内分泌性高血圧診療マニュアル (2010)
  • Author(s): 井上紘輔, 寺田典生
  • Total Pages: 3
  • Publisher: 診断と治療社
• [Book] 内分泌高血圧診療マニュアル (2010)
  • Author(s): 井上紘輔、寺田典生
  • Publisher: 診断と治療社