Cellular biology of podocytes as immunocompetent cells

• Project/Area Number: 21790817
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Yokohama City University
• Principal Investigator: NAGAHAMA Kiyotaka 横浜市立大学, 医学部, 助教 (00336538)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: ネフリン / 糸球体上皮細胞 / 糸球体障害 / cGVHD / Fcレセプター / 慢性GVHD

Research Abstract

Real-time PCR and confocal microscopy revealed expression of Fc receptor gamma chain in murine podocytes. Although their role in proteinuric state should be clarified, we also found that a cytokine and its receptor expression in podocytes especially in proteinuric state, suggesting that podocytes play a certain role in the inflammatory state associated with glomerulonephritis. Further study using conditional knockout mouse is warranted to dissect certain roles of the cytokine in the pathogenesis of podocyte in jury.

Research Products

• [Journal Article] Complexity of tumor vasculature and molecular targeting therapies (2011)
  • Author(s): Furuya M, Nagahama K, Ishizu A, Otsuka N, Nagashima Y, Aoki I.
  • Journal Title: Front Biosci(Elite Ed) Volume: 3 Pages: 549-61
• [Journal Article] Recovery of Renal Function in a Dialysis-dependent Patient with Microscopic Polyangiitis and both Myeloperoxidase Anti-neutrophil Cytoplasmic Antibodies and Anti-glomerular Basement Membrane Antibodies (2011)
  • Author(s): Mandai S, Nagahama K, Tsuura Y, Hirai T, Yoshioka W, Takahashi D, Aki S, Aoyagi M, Tanaka H, Tamura T.
  • Journal Title: Naika Volume: 50(15) Pages: 1599-603
  • NAID: 130000969356
• [Journal Article] Complexity of tumor vasculature and molecular targeting therapies (2011)
  • Author(s): Furuya M, Nagahama K, et.al
  • Journal Title: Front Biosci (Elite Ed) Volume: 3 Pages: 549-561
  • Peer Reviewed
• [Journal Article] Recovery of Renal Function in a Dialysis-dependent Patient with Microscopic Polyangiitis and both Myeloperoxidase Anti-neutrophil Cytoplasmic Antibodies and Anti-glomerular Basement Membrane Antibodies (2011)
  • Author(s): Mandai S, Nagahama K, et.al
  • Journal Title: Naika Volume: 50(15) Pages: 1599-1603
  • NAID: 130000969356
  • Peer Reviewed
• [Journal Article] A novel method for isolating podocytes using magnetic activated cell sorting (2010)
  • Author(s): Murakami A, Oshiro H, Kanzaki S, Yamaguchi A, Yamanaka S, Furuya M, Miura S, Kanno H, Nagashima Y, Aoki I, Nagahama K.
  • Journal Title: Nephrol Dial Transplant Volume: 25(12) Pages: 3884-90
• [Journal Article] Urinary cytologic features of primary large cell neuroendocrine carcinoma of the urinary bladder : a case report (2010)
  • Author(s): Oshiro H, Gomi K, Nagahama K, Nagashima Y, Kanazawa M, Kato J, Hatano T, Inayama Y.
  • Journal Title: Acta Cytol Volume: 54(3) Pages: 303-10
• [Journal Article] Cutaneous metastasis of pancreatic carcinoma as an initial symptom in the lower extremity with obstructive lymphedema treated by physiotherapy and lymphaticovenous shunt : a case report, review, and pathophysiological implications (2010)
  • Author(s): Shimizu H, Maegawa J, Ho T, Yamamoto Y, Mikami T, Nagahama K.
  • Journal Title: Lymphology Volume: 43(1) Pages: 19-24
• [Journal Article] Quiz page July 2010 : multiple lung nodules with worsening kidney function (2010)
  • Author(s): Tanaka H, Fujii T, Kusaura T, Kobayashi A, Oshiro H, Sasaki T, Yamaguchi Y, Nagahama K.
  • Journal Title: Am J Kidney Dis Volume: 56(1)
• [Journal Article] A novel method for isolating podocytes using magnetic activated cell sorting. (2010)
  • Author(s): A.Murakami, et al.
  • Journal Title: Nephrol Dial Transplant. Volume: 25 Pages: 3884-3890
  • Peer Reviewed
• [Journal Article] Cutaneous metastasis of pancreatic carcinoma as an initial symptom in the lower extremity with obstructive lymphedema treated by physiotherapy and lymphaticovenous shunt : a case report, review, and pathophysiological implications. (2010)
  • Author(s): H.Shimizu, et al.
  • Journal Title: Lymphology Volume: 43 Pages: 19-24
  • Peer Reviewed
• [Journal Article] Quiz page July 2010 : multiple lung nodules with worsening kidney function. (2010)
  • Author(s): H.Tanaka, et al.
  • Journal Title: Am J Kidney Dis Volume: 56
  • Peer Reviewed
• [Journal Article] Symptomatic gastric sarcoidosis with multiple antral ulcers (2009)
  • Author(s): Akiyama T, Endo H, Inamori M, Iida H, Hosono K, Fujita K, Yoneda M, Takahashi H, Goto A, Abe Y, Kirikoshi H, Kobayashi N, Kubota K, Saito S, Nagahama K, Inayama Y, Nakajima A.
  • Journal Title: Endoscopy Volume: 41 Pages: 2-159
• [Journal Article] Mizoribine for renal sarcoidosis : effective steroid tapering and prevention of recurrence (2009)
  • Author(s): Ito S, Harada T, Nakamura T, Imagawa T, Nagahama K, Sasaki T, Fujinaga S, Aihara Y, Yokota S.
  • Journal Title: Pediatr Nephrol Volume: 24(2) Pages: 411-4
• [Journal Article] Differential requirement for CARMA1 in agonist-selected T-cell development (2009)
  • Author(s): Medoff BD, Sandall BP, Landry A, Nagahama K, Mizoguchi A, Luster AD, Xavier RJ.
  • Journal Title: Eur J Immunol Volume: 39(1) Pages: 78-84
• [Journal Article] Differential requirement for CARMA1 in agonist-selected T-cell development. (2009)
  • Author(s): Medoff BD, et al
  • Journal Title: Eur J Immunol 39(1) Pages: 78-84
  • Peer Reviewed
• [Journal Article] Mizoribine for renal sarcoidosis : effective steroid tapering and prevention recurrence. (2009)
  • Author(s): Ito S, et al
  • Journal Title: Pediatr Nephrol 24(2) Pages: 411-414
  • Peer Reviewed
• [Presentation] 診断病理における制度管理:大腸癌における脈管侵襲評価における診断者間のばらつきについて (2011)
  • Author(s): 稲山嘉明, 田栗正隆, 山中正二, 中山崇, 平沙代子, 小野響子, 海野俊徳, 矢澤卓也, 下山田博明, 奥寺康司, 長嶋洋治, 古屋充子, 長濱清隆, 村上あゆみ, 青木一郎, 森田智視
  • Organizer: 第100会日本病理学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-04-30
• [Presentation] 子宮内膜症性嚢胞と卵巣癌:CXCケモカイン軸に特徴づけられる炎症性微小環境の変化 (2011)
  • Author(s): 古屋充子, 米山朋那, 宮城悦子, 上大介, 長濱清隆, 田中玲子, 太田一郎, 宮城洋平, 長嶋洋治, 平原史樹, 稲山嘉明, 青木一郎
  • Organizer: 第100会日本病理学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-04-30
• [Presentation] 診断病理における制度管理:大腸癌における脈管侵襲評価における診断者間のばらつきについて (2011)
  • Author(s): 稲山嘉明, 長濱清隆, 他
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-04-30
• [Presentation] 子宮内膜症性嚢胞と卵巣癌:CXCケモカイン軸に特徴づけられる炎症性微小環境の変化 (2011)
  • Author(s): 古屋充子, 長濱清隆, 他
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-04-30
• [Presentation] A novel method for isolating podocytes using magnetic activated cell sorting (2010)
  • Author(s): 長濱清隆, 村上あゆみ, 藩勤雅
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-06-16
• [Presentation] A novel method for isolating podocytes using magnetic activated cell sorting (2010)
  • Author(s): 長濱清隆, ら
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: 神戸国際会議場(兵庫)
  • Year and Date: 2010-06-16
• [Presentation] MACSを用いた糸球体上皮細胞の単離 (2010)
  • Author(s): 村上あゆみ, 潘勤雅, 大城久, 山中正二, 古屋充子, 長嶋洋治, 青木一郎, 長濱清隆
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京京王プラザ
  • Year and Date: 2010-04-28
• [Presentation] 卵巣腫瘍におけるIFN-γ誘導性ケモカイン受容体CXCR3バリアントの解析 (2010)
  • Author(s): 古屋充子, 米山朋那, 宮城悦子, 田中玲子, 上大介, 長濱清隆, 長嶋洋治, 宮城洋平, 稲山嘉明, 青木一郎
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京京王プラザ
  • Year and Date: 2010-04-28
• [Presentation] 多発性骨髄腫に合併した好酸球性多発性筋炎の剖検例 (2010)
  • Author(s): 大城久, 小野響子, 平沙代子, 海野俊徳, 渡辺眞一郎, 山中正二, 中山崇, 長嶋洋治, 長濱清隆, 村上あゆみ, 青木一郎, 稲山嘉明
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京京王プラザ
  • Year and Date: 2010-04-27
• [Presentation] 横浜市立大学におけるバイオバンク室の運営と課題 (2010)
  • Author(s): 稲山嘉明, 山中正二, 村上あゆみ, 中山崇, 大城久, 海野俊徳, 小野響子, 平沙代子, 矢澤卓也, 下山田博明, 奥寺康司, 長嶋洋治, 古屋充子, 長濱清隆, 北村均, 青木一郎
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京京王プラザ
  • Year and Date: 2010-04-27
• [Presentation] 副鼻腔原発lipid-rich carcinomaの細胞学的気所見. (2009)
  • Author(s): 大城久, 他
  • Organizer: 第48回日本臨床細胞学会秋期大会
  • Place of Presentation: JAL福岡リゾートシーフォークホテル
  • Year and Date: 2009-10-31
• [Presentation] 副鼻腔原発lipid-rich carcinomaの細胞学的所見 (2009)
  • Author(s): 大城久, 長嶋洋治, 金澤美千代, 佐川弘美, 西尾由紀子, 長濱清隆, 小野響子, 中山崇, 山中正二, 稲山嘉明
  • Organizer: 第48回日本臨床細胞学会秋季大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-10-30
• [Presentation] 体肺動脈短絡術20年後にチアノーゼ腎症と診断された一例 (2009)
  • Author(s): 村上あゆみ, 長濱清隆, 小林麻裕美, 平和伸仁
  • Organizer: 第39回日本腎臓学会東部学術大会
  • Place of Presentation: 東京グランドプリンスホテル新高輪
  • Year and Date: 2009-10-03
• [Presentation] 体肺動脈短絡術20年後にチアノーゼ腎症と診断された一例. (2009)
  • Author(s): 村上あゆみ, 他
  • Organizer: 第39回日本腎臓学会東部学術大会
  • Place of Presentation: グランドプリンスホテル新高輪 国際館パミール
  • Year and Date: 2009-10-03
• [Presentation] 尿細管間質性腎炎に膜性腎症、間質性肺炎を合併したIgG4関連疾患の一例. (2009)
  • Author(s): 藤井琢磨, 他
  • Organizer: 第39回日本腎臓学会東部学術大会
  • Place of Presentation: グランドプリンスホテル新高輪 国際館パミール
  • Year and Date: 2009-10-02
• [Presentation] A novel method for isolating podocyte utilizing magnetic cell sorting. (2009)
  • Author(s): 長濱清隆, 他
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-06-03
• [Presentation] WT1遺伝子のexon10のナンセンス変異を認め腎移植に至った1例 (2009)
  • Author(s): 村上あゆみ, 長濱清隆, 原田知典, 伊藤秀一, 寺西淳一, 江中牧子, 田辺美樹子, 河内香江, 佐々木毅, 野澤明典, 古屋充子, 長嶋洋治, 青木一郎
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都国立京都国際会館
  • Year and Date: 2009-05-03
• [Presentation] WT1遺伝子のexon10のナンセンス変異を認め腎移植に至った1例 (2009)
  • Author(s): 村上あゆみ, 他
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-05-03
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/~byori2/index.html
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/~byori2/index.html