Functional analysis of ERK signaling pathway in ERK1/2 kidney specific knockout mice

• Project/Area Number: 21790818
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: SUGIYAMA Noriyuki Kyoto Prefectural University of Medicine, 医学研究科, 助教 (90381954)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 腎臓学 / 嚢胞腎 / Erk / ノックアウトマウス / 急性腎不全 / 細胞内シグナル伝達系 / 細胞増殖

Research Abstract

This study is that analysis of Erk in kidney. We obtained that Erk functioned renal regeneration after ischemia-refusion.
The next study indicates that random oriented cell division is an initial event for renal tubule expansion and precedes cell proliferation in inv mutant kidneys. However, our results do not support the hypothesis that the canonical Wnt signaling pathway is involved in renal cyst development of inv mutant mice.

Research Products

• [Journal Article] The canonical Wnt signaling pathway is not involved in renal cyst development in the kidneys of inv mutant mice. (2011)
  • Author(s): Sugiyama N, Tsukiyama, T Yamaguchi TP, Yokoyama T.
  • Journal Title: Kidney Int. 79(9) Pages: 957-965
  • Peer Reviewed
• [Journal Article] 線毛と嚢胞腎 (2011)
  • Author(s): 杉山紀之
  • Journal Title: 腎と透析 70(2) Pages: 237-242
• [Journal Article] 繊毛と嚢胞腎 (2011)
  • Author(s): 杉山紀之
  • Journal Title: 腎と透析 Volume: 2 Pages: 237-242
• [Journal Article] Abnormal epithelial cell polarity and ectopic epidermal growth factor receptor (EGFR) expression induced in Emx2 KO embryonic gonads. (2011)
  • Author(s): Kusaka, M.
  • Journal Title: Endocrinology Volume: 151 Pages: 5893-904
  • Peer Reviewed
• [Journal Article] Abnormal Epithelial Cell Polarity and Ectopic Epidermal Growth Factor Receptor (EGFR) Expression Induced in Emx2 KO Embryonic Gonads. (2010)
  • Author(s): Kusaka M, Katoh-Fukui Y, Ogawa H, Miyabayashi K, Baba T, Shima Y, Sugiyama N, Sugimoto Y, Okuno Y, Kodama R, Iizuka-Kogo A, Senda T, Sasaoka T, Kitamura K, Aizawa S, Morohashi KI.
  • Journal Title: Endocrinology. 151(12) Pages: 5893-5904
  • NAID: 120005227384
  • Peer Reviewed
• [Journal Article] ERK regulates renal cell proliferation and renal cyst expansion in inv mutant mice. (2009)
  • Author(s): Okumura Y, Sugiyama N, Tanimura S, Nishida M, Hamaoka K, Kohno M, Yokoyama T.
  • Journal Title: Acta Histochem Cytochem. 42(2) Pages: 39-45
  • NAID: 130000114963
  • Peer Reviewed
• [Journal Article] 嚢胞性腎疾患と繊毛 (2009)
  • Author(s): 横山尚彦、杉山紀之、芝大
  • Journal Title: 細胞工学 28(10) Pages: 1021-1026
• [Presentation] Renal cystic diseases as a cilliopathy (2011)
  • Author(s): Yokoyama, T., Sugiyama, N., Shiba,D., Fukui, H., Nakata, K.
  • Organizer: 第116回解剖学会全国学術集会 抄録 The Journal of Physiological Sciences 61(suppl): s78(2011)
  • Place of Presentation: 横浜
• [Presentation] 嚢胞腎疾患と一次線毛 (2010)
  • Author(s): 杉山紀之
  • Organizer: 第13回埼玉若手腎臓病若手研究会 招待講演
  • Place of Presentation: 大宮 パレスホテル大宮
  • Year and Date: 2010-12-11
• [Presentation] 嚢胞性腎疾患と一次線毛 (2010)
  • Author(s): 杉山紀之
  • Organizer: 第13回埼玉若手腎臓病若手研究会
  • Place of Presentation: パレスホテル大宮(埼玉)
  • Year and Date: 2010-12-11
• [Presentation] 繊毛と嚢胞腎 (2010)
  • Author(s): 杉山紀之
  • Organizer: 第32回腎臓セミナー 招待講演
  • Place of Presentation: 東京 海運ビル
  • Year and Date: 2010-08-28
• [Presentation] 繊毛と嚢胞腎 (2010)
  • Author(s): 杉山紀之
  • Organizer: 第32回腎臓セミナー
  • Place of Presentation: 海運ホテル(東京)
  • Year and Date: 2010-08-28
• [Presentation] Defective planar cell polarity/noncanonical Wnt signaling pathway and normal canonical Wnt/β-catenin signaling pathway in inv mice (2010)
  • Author(s): Sugiyama, N., Yokoyama, T.
  • Organizer: Keystone Symposia on Molecular and Cellular Biology "Cilia, Signaling and Human Disease"
  • Place of Presentation: Portola Hotel & Spa, Monterey, California
  • Year and Date: 2010-02-23
• [Presentation] Defective planar cell polarity/non-canonical Wnt signaking pathway and normal canonical Wnt/β-catenin signaling pathway in inv mice (2010)
  • Author(s): Sugiyama N, Yokoyama T
  • Organizer: Keystone Symposia on Molecular and Cellular Biology. "Cilia, Signaling and Human Disease"
  • Place of Presentation: Portola Hotel & Spa(California, USA)
  • Year and Date: 2010-02-23
• [Presentation] 尿細管形態維持装置としての繊毛タンパクInvの機能 (2009)
  • Author(s): 横山尚彦、杉山紀之、芝大
  • Organizer: 第32回日本分子生物学会年会Workshop
  • Place of Presentation: 神奈川県・パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] 尿細管形態維持装置としての繊毛蛋白Invの機能 (2009)
  • Author(s): 横山尚彦、杉山紀之、芝大
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-11
• [Presentation] ERK Regulates Renal Cell Proliferation and Renal Cyst Expansion in inv Mutant Mice (2009)
  • Author(s): 杉山紀之、奥村保子、横山尚彦
  • Organizer: 第50回日本組織細胞化学会
  • Place of Presentation: 滋賀・ピアザ淡海
  • Year and Date: 2009-09-26
• [Presentation] ERK Regulates Renal Cell Proliferation and Renal Cyst Expansion in inv Mutant Mice (2009)
  • Author(s): 奥村保子、杉山紀之、横山尚彦
  • Organizer: 日本組織細胞化学会
  • Place of Presentation: ピアザ淡海(滋賀県)
  • Year and Date: 2009-09-26
• [Presentation] invマウスの嚢胞腎におけるcanonical Wntシグナル経路の関与 (2009)
  • Author(s): 杉山紀之、横山尚彦
  • Organizer: 第52回日本腎臓学会
  • Place of Presentation: 神奈川県・パシフィコ横浜
  • Year and Date: 2009-06-04
• [Presentation] invマウスの嚢胞腎におけるcanonical Wntシグナル経路の関与 (2009)
  • Author(s): 杉山紀之、横山尚彦
  • Organizer: 日本腎臓学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-06-04
• [Remarks] ホームページ等
  • URL: http://www.f.kpu-m.ac.jp/k/anat2/Projects/themal.html