Changing of intracellular trafficking in polarized cells and regulation of serum urate level by the splicing variants of urate transporter URATv1.

• Project/Area Number: 21790819
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Kyorin University
• Principal Investigator: KIMURA Toru Kyorin University, 医学部, 助教 (30433725)
• Co-Investigator(Renkei-kenkyūsha): ANZAI Naohiko 杏林大学, 医学部, 准教授 (70276054)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 尿酸 / トランスポーター / 腎臓 / 極性発現 / 細胞内極性輸送

Research Abstract

It has been reported that two splicing variants of URATv1 are exist. One is the short isoform (URATv1-S) that composed with 512 amino acids and the other one is the long isoform (URATv1-L) that composed with 540 amino acids. URATv1-S was expressed in apical side of renal distal tubules. On the other hand, URATv1-L was expressed in basolateral side of renal proximal tubules.GFP fused URATv1s were expressed in MDCK cells and intracellular localization was observed to analyze trafficking mechanism in vitro. It seems that N-terminal of URATv1-L includes sorting signal to basolateral membrane.

Research Products

• [Journal Article] Functional Analysis of Human Sodium-Phosphate Transporter 4 (NPT4/SLC17A3) Polymorphisms. (2011)
  • Author(s): Jutabha P, Anzai N, Kimura T, Taniguchi A, Urano W, Yamanaka H, Endou H, Sakurai H.
  • Journal Title: J Pharmacol Sci. 115 Pages: 249-253
  • NAID: 10029892290
  • Peer Reviewed
• [Journal Article] Human sodium-phosphate transporter 4 (hNPT4/SLC17A3) as a common renal secretory pathway for drugs and urate. (2010)
  • Author(s): Jutabha P, Anzai N, Kitamura K, Taniguchi A, Kaneko S, Yan K, Yamada H, Shimada H, Kimura T, Katada T, Fukutomi T, Tomita K, Urano W, Yamanaka H, Seki G, Fujita T, Moriyama Y, Yamada A, Uchida S, Wempe MF, Endou H, Sakurai H.
  • Journal Title: J Biol Chem. 285 Pages: 35123-35132
  • Peer Reviewed
• [Journal Article] 尿酸トランスポーターURAT1トランスジェニックマウスにおける尿酸の体内動態 (2010)
  • Author(s): 塚田愛、木村徹、Promsuk Jutabha、安西尚彦、市田公美、櫻井裕之
  • Journal Title: 痛風と核酸代謝 34 Pages: 171-178
  • NAID: 10027722204
• [Journal Article] Functional Analysis of Human Organic Cation Transporter OCT3 (SLC22A3) Polymorphisms. (2010)
  • Author(s): Sakata T, Anzai N, Kimura T, et al.
  • Journal Title: J Pharmacol Sci. Volume: 113 Pages: 263-266
  • NAID: 10029889401
  • Peer Reviewed
• [Journal Article] Human sodium-phosphate transporter 4 (hNpT4/SLC17A3) as a commonrenal secretory pathway for drugs and urate. (2010)
  • Author(s): Jutabha P, Anzai N, Kitamura K, Taniguchi A, Kaneko S, Yan K, Yamada H, Shimada H, Kimura T, et al.
  • Journal Title: J Biol Chem. Volume: 285 Pages: 35123-35132
  • Peer Reviewed
• [Journal Article] 新規尿酸排出トランスポーターURATv1の尿酸輸送特性の解析 (2009)
  • Author(s): 木村徹、安西尚彦、Promsuk Jutabha、三浦大作、遠藤仁、櫻井裕之
  • Journal Title: 痛風と核酸代謝 33 Pages: 177-182
  • NAID: 10025596953
  • Peer Reviewed
• [Journal Article] 腎臓の尿酸トランスポーター:最近の進歩 (2009)
  • Author(s): 安西尚彦、Promsuk Jutabha、木村徹、櫻井裕之
  • Journal Title: 痛風と核酸代謝 33 Pages: 7-15
  • NAID: 10025596817
• [Journal Article] 新規尿酸排出トランスポータ-URArv1の尿酸輸送特性の解析 (2009)
  • Author(s): 木村徹、安西尚彦、Promsuk Jutabha、三浦大作、遠藤仁、櫻井裕之
  • Journal Title: 痛風と核酸代謝 33 Pages: 177-182
  • Peer Reviewed
• [Presentation] Elucidation of urate transport mechanism by analysis of renal urate transporters' transgenic mice. (2011)
  • Author(s): Toru Kimura
  • Organizer: 14th International Symposium on Purine and Pyrimidine Metabolism in Man (PP11)
  • Place of Presentation: 新宿
  • Year and Date: 2011-02-19
• [Presentation] 尿酸トランスポーターURATv1/GLUT9の組織発現と極性細胞におけるソーティングの解析 (2011)
  • Author(s): 木村徹
  • Organizer: 第44回日本痛風・核酸代謝学会総会
  • Place of Presentation: 新宿
  • Year and Date: 2011-02-19
• [Presentation] Isoform specific tissue distribution of URATv1/GLUT9 and their trafficking in the polarized cells. (2010)
  • Author(s): Toru Kimura
  • Organizer: 50^<th> The American Society for Cell Biology Annual Meeting
  • Place of Presentation: アメリカ フィラデルフィア
  • Year and Date: 2010-12-13
• [Presentation] Isoform specific tissue distribution of URATv1/GLUT9 and their trafficking in the polarized cells. (2010)
  • Author(s): Toru Kimura, Anzai Naohiko, Hiroyuki Sakurai
  • Organizer: 50^<th> The American Society for Cell Biology Annual Meeting
  • Place of Presentation: アメリカフィラデルフィア
  • Year and Date: 2010-12-13
• [Presentation] 尿酸輸送体過剰発現マウスを用いた尿酸輸送機構の解析 (2010)
  • Author(s): 木村徹
  • Organizer: 第53回日本腎臓学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-06-16
• [Presentation] 尿酸輸送機構の解明を目的とした尿酸トランスポーター過剰発現マウスの解析 (2010)
  • Author(s): 木村徹
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-03-17