Establishment of objective evaluation of neuropathic pain based on axonal ionic pathophysiology

• Project/Area Number: 21790834
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Chiba University
• Principal Investigator: MISAWA Sonoko Chiba University, 大学院・医学研究院, 助教 (30375753)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 軸索機能検査法 / 軸索Na電流 / 神経原性疼痛 / 糖尿病性末梢神経障害 / Naチャネル阻害薬

Research Abstract

Peripheral nerve injury alters function and expression of voltage gated Na^+ channels on the axolemma, leading to ectopic firing and neuropathic pain. We investigated changes in nodal persistent Na^+ currents in human sensory axons using computerized threshold tracking system. Neuropathic pain was significantly associated with increased Na^+ currents and was suppressed by administration of Na^+ channel blocker associated with decreased Na^+ currents. Objective degree of pain could be evaluated by threshold tracking system.

Research Products

• [Journal Article] Distal motor axonal dysfunction in amyotrophic lateral sclerosis. (2011)
  • Author(s): Noto Y, Kanai K, Misawa S, Shibuya K, Isose S, Nasu S, Sekiguchi Y, Fujimaki Y, Nakagawa M, Kuwabara S.
  • Journal Title: J Neurol Sci. 15;302(1-2) Pages: 58-62
  • Peer Reviewed
• [Journal Article] Elevated CSF TDP-43 levels in amyotrophic lateral sclerosis : specificity, sensitivity, and a possible prognostic value. (2011)
  • Author(s): Noto Y, Shibuya K, Sato Y, Kanai K, Misawa S, Sawai S, Mori M, Uchiyama T, Isose S, Nasu S, Sekiguchi Y, Fujimaki Y, Kasai T, Tokuda T, Nakagawa M, Kuwabara S.
  • Journal Title: Amyotroph Lateral Scler. 12(2) Pages: 140-143
  • Peer Reviewed
• [Journal Article] Sensorimotor manifestations without autonomic symptoms in two siblings with TTR Va1107 familial amyloid polyneuropathy. (2011)
  • Author(s): Asahina M, Akaogi Y, Misawa S, Kanai K, Ando Y, Sakakibara R, Arai K, Hattori T, Kuwabara S.
  • Journal Title: Clin Neurol Neurosurg. 113(2) Pages: 139-141
  • Peer Reviewed
• [Journal Article] Altered axonal excitability properties in juvenile muscular atrophy of distal upper extremity (Hirayama disease). (2011)
  • Author(s): Sawai S, Misawa S, Kanai K, Isose S, Shibuya K, Noto Y, Fujimaki Y, Sekiguchi Y, Nasu S, Nomura F, Kuwabara S.
  • Journal Title: Clin Neurophysiol. 122(1) Pages: 205-259
  • Peer Reviewed
• [Journal Article] POEMS syndrome with Guillan-Barre syndrome-like acute onset : a case report and review of neurological progression in 30 cases. (2011)
  • Author(s): Isose S, Misawa S, Kanai K, Shibuya K, Sekiguchi Y, Nasu S, Fujimaki Y, Noto Y, Nakaseko C, Kuwabara S.
  • Journal Title: J Neurol Neurosurg Psychiatry. in press
  • Peer Reviewed
• [Journal Article] POEMS syndrome with Guillan-Barre syndrome-like acute onset : a case report and review of neurological progression in 30 cases. (2011)
  • Author(s): Isose S, Misawa S, et al.
  • Journal Title: J Neurol Neurosurg Psychiatry Volume: (In press)
  • Peer Reviewed
• [Journal Article] Neuromuscular transmission is not impaired in axonal Guillain-Barre syndrome. (2011)
  • Author(s): Kuwabara S, Kokubun N, Misawa S, et al.
  • Journal Title: J Neurol Neurosurg Psychiatry Volume: (In press)
  • Peer Reviewed
• [Journal Article] Spinal myoclonus in the periscapular muscles after mastectomy assessed by FDG-PET. (2010)
  • Author(s): Misawa S, Hayakawa S, Himuro K, Sawai S, Akaogi Y, Ito S, Kuwabara S.
  • Journal Title: Clin Neurol Neurosurg. 112(6) Pages: 527-529
  • Peer Reviewed
• [Journal Article] Multifocal conduction blocksin sarcoid peripheral neuropathy. (2010)
  • Author(s): Sawai S, Misawa S, Kobayashi M, Kanai K, Isose S, Shibuya K, Sekiguchi Y, Nasu S, Noto Y, Fujimaki Y, Koga S, Ohtani R, Kuwabara S.
  • Journal Title: Intern Med. 49(5) Pages: 471-474
  • Peer Reviewed
• [Journal Article] Mexiletine suppresses nodal persistent sodium currents in sensory axons of patients with neuropathic pain. (2010)
  • Author(s): Isose S, Misawa S, Sakurai K, Kanai K, Shibuya K, Sekiguchi Y, Nasu S, Noto Y, Fujimaki Y, Yokote K, Kuwabara S.
  • Journal Title: Clin Neurophysiol. 121(5) Pages: 719-724
  • Peer Reviewed
• [Journal Article] Long-term regular plasmapheresis as a maintenance treatment for chronic inf-ammatory demyelinating polyneuropathy. (2010)
  • Author(s): Isose S, Mori M, Misawa S, Shibuya K, Kuwabara S.
  • Journal Title: J Peripher Nery Syst. 15(2) Pages: 147-149
  • Peer Reviewed
• [Journal Article] Spinal myoclonus in the periscapular muscles after mastectomy assessed by FDG-PET. (2010)
  • Author(s): Misawa S, et al.
  • Journal Title: Clin Neurol Neurosurg Volume: 112 Pages: 527-529
  • Peer Reviewed
• [Journal Article] Mexiletine suppresses nodal persistent sodium currents in sensory axons of patients with neuropathic pain. (2010)
  • Author(s): Isose S, Misawa S, et al.
  • Journal Title: Clin Neurophyisiol Volume: 122 Pages: 205-209
  • Peer Reviewed
• [Journal Article] Mexiletine suppresses nodal persistent sodium currents in sensory axons of patients with neuropathic pain (2010)
  • Author(s): Isose S, et al.
  • Journal Title: Clin Neurophyisiol 121 Pages: 719-724
  • Peer Reviewed
• [Journal Article] Neuropathic pain is associated with increased nodal persistent Na(+) currents in human diabetic neuropathy. (2009)
  • Author(s): Misawa S, Sakurai K, Shibuya K, Isose S, Kanai K, Ogino J, Ishikawa K, Kuwabara S.
  • Journal Title: J Peripher Nerv Syst. 14(4) Pages: 279-284
  • Peer Reviewed
• [Journal Article] Chronic inflammatory demyelinating polyneuropathy sera inhibitaxonal growth of mouse dorsal root ganglion neurons by activation of Rho-kinase. (2009)
  • Author(s): Taniguchi J, Sawai S, Mori M, Kubo T, Kanai K, Misawa S, Isose S, Yamashita T, Kuwabara S.
  • Journal Title: Ann Neurol. 66(5) Pages: 694-697
  • Peer Reviewed
• [Journal Article] Relapse of POEMS syndrome without increased level of VEGF. (2009)
  • Author(s): Kuwabara S, Kanai K, Misawa S, Nakaseko C.
  • Journal Title: Neuromuscul Disord. 19(10)
  • Peer Reviewed
• [Journal Article] Tokyo Metropolitan Neuromuscular Electrodiagnosis Study Group. Utility of the distal compound muscle action potential duration for diagnosis of demyelinating neuropathies. (2009)
  • Author(s): Isose S, Kuwabara S, Kokubun N, Sato Y, Mori M, Shibuya K, Sekiguchi Y, Nasu S, Fujimaki Y, Noto Y, Sawai S, Kanai K, Hirata K, Misawa S
  • Journal Title: J Peripher Nery Syst. 14(3) Pages: 151-158
  • Peer Reviewed
• [Journal Article] The effects of age, gender, and body mass index on amplitude of sensory nerve action potentials : multivariate analyses. (2009)
  • Author(s): Fujimaki Y, Kuwabara S, Sato Y, Isose S, Shibuya K, Sekiguchi Y, Nasu S, Noto Y, Taniguchi J, Misawa S.
  • Journal Title: Clin Neurophysiol. 120(9) 39(1)
  • Peer Reviewed
• [Journal Article] Successful combination treatment with bevacizumab, thalidomide and autologous PBSC for severe POEMS syndrome. (2009)
  • Author(s): Ohwada C, Nakaseko C, Sakai S, Takeda Y, Abe D, Takeuchi M, Sakaida E, Masuda S, Shimizu N, Cho R, Nishimura M, Kanai K, Misawa S, Kuwabara S.
  • Journal Title: Bone Marrow Transplant. 43(9) Pages: 739-740
  • Peer Reviewed
• [Journal Article] Differences in excitability properties of FDI and ADM motor axons. (2009)
  • Author(s): Bae JS, Sawai S, Misawa S, Kanai K, Isose S, Kuwabara S.
  • Journal Title: Muscle Nerve. 39(3) Pages: 350-354
  • Peer Reviewed
• [Journal Article] Tokyo Metropolitan Neuromuscular Electrodiagnosis Study Group. Utility of trapezius EMG for diagnosis of amyotrophic lateral sclerosis. (2009)
  • Author(s): Sonoo M, Kuwabara S, Shimizu T, Komori T, Hirashima F, Inaba A, Hatanaka Y, Misawa S, Kugio Y
  • Journal Title: Muscle Nerve.
• [Journal Article] Neuropathic pain is associated with increased nodal persistent Na^+ currents in human diabetic neuropathy (2009)
  • Author(s): Misawa S, et al.
  • Journal Title: J Peripher Nerv Syst 14 Pages: 279-284
  • Peer Reviewed
• [Journal Article] Chronic inflammatory demyelinating polyneuropathy sera inhibit axonal growth of mouse dorsal root ganglion neurons by activation of Rho-kinase (2009)
  • Author(s): Taniguchi J, et al.
  • Journal Title: Ann Neurol. 66 Pages: 694-697
  • Peer Reviewed
• [Journal Article] Utility of the distal compound muscle action potential duration for diagnosis of demyelinating neuropathies (2009)
  • Author(s): Isose S, et al.
  • Journal Title: J Peripher Nerv Syst 14 Pages: 151-158
  • Peer Reviewed
• [Journal Article] The effects of age, gender, and body mass index on amplitude of sensory nerve action potentials : multivariate analyses (2009)
  • Author(s): Fujimaki, et al.
  • Journal Title: Clin Neurophyisiol 120 Pages: 1683-1686
  • Peer Reviewed
• [Journal Article] Differences in excitability properties of FDI and ADM motor axons (2009)
  • Author(s): Bae JS, et al.
  • Journal Title: Muscle Nerve 39 Pages: 350-354
  • Peer Reviewed
• [Journal Article] Neuromuscular transmission is not impaired in axonal Guillain-Barre syndrome.
  • Author(s): Kuwabara S, Kokubun N, Misawa S, Kanai K, Isose S, Shibuya K, Noto Y, Mori M, Sekiguchi Y, Nasu S, Fujimaki Y, Hirata K, Yuki N.
  • Journal Title: J Neurol Neurosurg Psychiatry. In press
  • Peer Reviewed
• [Presentation] Electrophysiology in hemifacial spasm (2010)
  • Author(s): Misawa S.
  • Organizer: 29th.International Congress of Clinical Neurophysiology、Workshop
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-10-31
• [Presentation] Electrophysiology in Hemifcialspasm (2010)
  • Author(s): Misawa S.
  • Organizer: International Congress of Clinical Neurophysiology
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-10-31
• [Presentation] Ionic pathophysiology of human diabetic neuropathy (2010)
  • Author(s): Misawa S.
  • Organizer: 29th.International Congress of Clinical Neurophysiology、Symposium
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-10-30
• [Presentation] Ionic Pathophysiology in Diabetic Neuropathy (2010)
  • Author(s): Misawa S.
  • Organizer: International Congress of Clinical Neurophysiology
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-10-30
• [Presentation] Muscle ultrasonography : A novel diagnostic approach to detect fasciculations in amyotrphic lateral sclerosis (2010)
  • Author(s): Misawa S.
  • Organizer: International Congress of Clinical Neurophysiology
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-10-29
• [Presentation] 軸索イオンチャネル機能モニタリングに基づく糖尿病性神経障害治療 (2010)
  • Author(s): 三澤園子
  • Organizer: 第25回日本糖尿病合併症学会、シンポジウム
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2010-10-23
• [Presentation] Crow-Fukase (POEMS)症候群におけるサリドマイド療法と移植療法:稀少疾病における治療のエビデンス確立をめざして (2010)
  • Author(s): 三澤園子
  • Organizer: 第28回日本神経治療学会総会、シンポジウム
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2010-07-15
• [Presentation] Crow-Fukase症候群に対するサリドマイド療法と移植療法 (2010)
  • Author(s): 三澤園子
  • Organizer: 日本神経治療学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-07-15
• [Presentation] 神経伝導検査の基礎と臨床:パターンで考える神経伝導検査所見 (2009)
  • Author(s): 三澤園子
  • Organizer: 第39回日本臨床神経生理学会学術集会、教育セミナー
  • Place of Presentation: 福岡県北九州市
  • Year and Date: 2009-11-18
• [Presentation] 神経伝導検査の基礎と臨床 (2009)
  • Author(s): 三澤園子
  • Organizer: 臨床神経生理学会
  • Place of Presentation: 小倉周際会議場
  • Year and Date: 2009-11-17