Study on a molecular basis for the cytotoxicity induced by polyglutamine proteins.
| Project/Area Number |
21790837
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Niigata University |
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Principal Investigator |
TAKAHASHI Toshiaki Niigata University, 医歯学系, 助教 (70377191)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 神経変性 / ポリグルタミン病 / モノマー / オリゴマー / FRET / 重合体 / 細胞膜 / シリンダー構造 |
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| Research Abstract |
To assess potential a conformational change of an expanded polyglutamine monomer in living cells, I used single molecular fluorescence resonance energy transfer (FRET) methods in living cells. On the basis of the FRET microscopic observation, a polyglutamine monomer forms a cylindrical structure. However, these cylindrical monomers had no cytotoxicity in neuronally differentiated cells. Furthermore, it was shown that polyglutamine oligomers induced greater cytotoxicity than these polyglutamine monomers. These findings support the hypothesis that polyglutamine oligomers have an important initiation of cytotoxicities.
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Report
(3 results)
Research Products
(6 results)
