Induction of brown adipogenesis of muscle-derived stem cells

• Project/Area Number: 21790884
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Metabolomics
• Research Institution: Fujita Health University
• Principal Investigator: UEZUMI Akiyoshi 藤田保健衛生大学, 総合医科学研究所, 助教 (60434594)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 褐色脂肪細胞 / 骨格筋 / 間葉系幹細胞 / 間葉系前駆細胞 / メタボリックシンドローム

Research Abstract

Generation of new brown adipocytes in adult provides a new therapeutic strategy against obesity. We identified mesenchymal stem cells in adult skeletal muscle and showed that these cells possess high brown adipogenic differentiation potential. Muscle-derived mesenchymal stem cells have an independent developmental origin from a classic brown adipose tissue. We also identified these mesenchymal stem cells in adult human skeletal muscle. Finally, we established efficient gene transduction system that enables highly regulated gene expression in mesenchymal stem cells.

Research Products

• [Journal Article] Fibrosis and adipogenesis originate from common mesenchymal progenitor in skeletal muscle (2011)
  • Author(s): Uezumi A, Ito T, Morikawa D, Shimizu N, Yoneda T, Segawa M, Yamaguchi M, Ogawa R, Milkov M, Miyagoe-Suzuki Y, Takeda S, Tsujikawa K, Tsuchida K, Yamamoto H, Fukada S
  • Journal Title: J. Cell Sci. Volume: 124(21) Pages: 3654-3664
  • Peer Reviewed
• [Journal Article] Fibrosis and adipogenesis originate from a common mesenchymal progenitor in skeletal muscle (2011)
  • Author(s): Uezumi A, Ito T, Morikawa D, Shimizu N, Yoneda T, Segawa M, Yamaguchi M, Ogawa R, Matev MM, Miyagoe-Suzuki Y, Takeda S, Tsujikawa K, Tsuchida K, Yamamoto H, Fukada S
  • Journal Title: J Cell Sci Volume: 124(21) Issue: 21 Pages: 3654-3664
  • DOI: 10.1242/jcs.086629
  • Peer Reviewed
• [Journal Article] Hesr1 and Hesr3 are essential to generate undifferentiated quiescent satellite cells and to maintain satellite cell numbers (2011)
  • Author(s): F ukada S, Yamaguchi M, Kokubo H, Ogawa R, Uezumi A, Yoneda T, Matev MM, Motohashi N, Ito T, Zolkiewska A, Johnson RL, Saga Y, Miyagoe-Suzuki Y, Tsujikawa K, Takeda S, Yamamoto H
  • Journal Title: Development Volume: 138 Issue: 21 Pages: 4609-4619
  • DOI: 10.1242/dev.067165
  • Peer Reviewed
• [Journal Article] 骨格筋内脂肪形成のメカニズム (2011)
  • Author(s): 上住聡芳
  • Journal Title: Modern Physician Volume: 31(11) Pages: 1313-1315
• [Journal Article] 骨格筋内在性の間葉系前駆細胞 (2011)
  • Author(s): 上住聡芳
  • Journal Title: 生体の科学 Volume: 62(2) Pages: 157-160
• [Journal Article] 骨格筋内脂肪の形成に寄与する間葉系前駆細胞の同定 (2011)
  • Author(s): 上住聡芳
  • Journal Title: 日本老年医学会雑誌 Volume: 48(2) Pages: 99-103
  • NAID: 10030469170
• [Journal Article] Mesenchymal progenitors distinct from satellite cells contribute to ectopic fat cell formation in skeletal muscle (2010)
  • Author(s): Uezumi A, Fukada S, Yamamoto N, Takeda S, Tsuchida K
  • Journal Title: Nat. Cell Biol Volume: 12(2) Pages: 143-152
  • Peer Reviewed
• [Journal Article] 老化や疾患における骨格筋の萎縮と治療への応用 (2010)
  • Author(s): 上住聡芳、土田邦博, 他
  • Journal Title: Biomedical Gerontology Volume: 34(4) Pages: 5-11
  • NAID: 10027470268
• [Journal Article] Mesenchymal progenitors distinct from satellite cells contribute to ectopic fat cell formation in skeletal muscle (2010)
  • Author(s): Uezumi A.et al.
  • Journal Title: Nature Cell Biology 12 Pages: 143-152
  • Peer Reviewed
• [Presentation] Roles for nonmyogenic mesenchymal progenitors in skeletal muscle regeneration (2012)
  • Author(s): Uezumi A.
  • Organizer: Keystone Symposia
  • Place of Presentation: California, USA
  • Year and Date: 2012-03-14
• [Presentation] Roles for nonmyogenic mesenchymal progenitors in skeletal muscle regeneration (2012)
  • Author(s): 上住聡芳
  • Organizer: Keystone Symposia
  • Place of Presentation: Olympic Valley, CA, USA
  • Year and Date: 2012-03-14
• [Presentation] Interaction between non-myogenic mesenchymal progenitors and muscle cells (2011)
  • Author(s): 上住聡芳
  • Organizer: 第34回日本分子生物学会年会ワークショップ
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] Interaction between non-myogenic mesenchymal progenitors and muscle cells (2011)
  • Author(s): 上住聡芳
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)(招待講演)
  • Year and Date: 2011-12-16
• [Presentation] 間葉系前駆細胞による筋再生制御機構の解析 (2011)
  • Author(s): 上住聡芳
  • Organizer: 精神・神経疾患研究開発費筋ジストロフィーに対するトランスレーショナル・リサーチ平成23年度研究班会議
  • Place of Presentation: JA共済ビル(東京都)(招待講演)
  • Year and Date: 2011-12-02
• [Presentation] 骨格筋の脂肪化や線維化に寄与する間葉系前駆細胞の同定 (2011)
  • Author(s): 上住聡芳
  • Organizer: 第11回徳島Bone Forum講演会
  • Place of Presentation: ホテルクレメント徳島(徳島県)(招待講演)
  • Year and Date: 2011-12-01
• [Presentation] 骨格筋の脂肪化や線維化に寄与する間葉系前駆細胞の同定 (2011)
  • Author(s): 上住聡芳
  • Organizer: 第29回日本骨代謝学会学術集会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2011-07-29
• [Presentation] 骨格筋の脂肪化や線維化に寄与する間葉系前駆細胞の同定 (2011)
  • Author(s): 上住聡芳
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)(招待講演)
  • Year and Date: 2011-07-29
• [Presentation] 骨格筋の脂肪変性のメカニズム (2011)
  • Author(s): 上住聡芳
  • Organizer: 第11回抗加齢医学会総会シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2011-05-27
• [Presentation] 骨格筋の脂肪変性のメカニズム (2011)
  • Author(s): 上住聡芳
  • Organizer: 第11回抗加齢医学会総会
  • Place of Presentation: 国立京都国際会館(京都府)(招待講演)
  • Year and Date: 2011-05-27
• [Presentation] Mesenchymal progenitors distinct from muscle satellite cells contribute to ectopic fat cell formation in skeletal muscle (2011)
  • Author(s): Uezumi A.
  • Organizer: Keystone Symposia
  • Place of Presentation: New Mexico, USA
  • Year and Date: 2011-02-02
• [Presentation] Mesenchymal progenitors distinct from muscle satellite cells contribute to ectopic fat cell formation in skeletal muscle (2011)
  • Author(s): 上住聡芳
  • Organizer: Keystone Symposia
  • Place of Presentation: Santa Fe, New Mexico, USA
  • Year and Date: 2011-02-02
• [Presentation] Mesenchymal progenitors distinct from muscle satellite cells contribute to ectopic fat formation in skeletal muscle (2009)
  • Author(s): Uezumi A.
  • Organizer: A joint meeting of Frontier in Myogenesis and Skeletal Muscle Stem and Satellite Cells
  • Place of Presentation: New York, USA
  • Year and Date: 2009-06-01
• [Presentation] Mesenchymal progenitors distinct from muscle satellite cells contribute to ectopic fat formation in skeletal muscle (2009)
  • Author(s): Uezumi A.
  • Organizer: 第7回幹細胞シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-05-16