A study on the mechanisms of ghrelin production and secretion by using a novel ghrelin-producing cell line.

• Project/Area Number: 21790891
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Kyoto University
• Principal Investigator: IWAKURA Hiroshi Kyoto University, 医学研究科, 助教 (20378615)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 内分泌学 / グレリン / 細胞株 / Ghrelin O-acyltrasferase / インスリン / ソマトスタチン / Ghrelin O-acyltransferase

Research Abstract

In this study, we developed a ghrelin producing cell line MGN3-1, which secretes high amount of ghrelin with physiological acyl-modification and maturation under the physiological regulation, making this cell line one of the best tools to study the mechanisms of ghrelin production and secretion in vitro. Further, we examined the effects of peptide hormones and neurotransmitters on ghrelin secretion from MGN3-1 cells and found that oxytocin, epinephrine and dopamine significantly stimulated ghrelin secretion from MGN3-1 cells in vitro.

Research Products

• [Journal Article] Generation of transgenic mice overexpressing a ghrelin analog. (2010)
  • Author(s): Yamada G, Ariyasu H, Iwakura H, Hosoda H, Akamizu T, Nakao K, Kangawa K.
  • Journal Title: Endocrinology. 151(12) Pages: 5935-5940
  • Peer Reviewed
• [Journal Article] Ghrelin and functional dyspepsia. (2010)
  • Author(s): Akamizu T, Iwakura H, Ariyasu H, Kangawa K.
  • Journal Title: Int J Pept.2010 Pages: 548457-548457
• [Journal Article] Molecular characterization of tumors from a transgenic mouse adrenal tumor model : comparison with human pheochromocytoma. (2010)
  • Author(s): Hattori Y, Kanamoto N, Kawano K, Iwakura H, Sone M, Miura M, Yasoda A, Tamura N, Arai H, Akamizu T, Nakao K, Maitani Y.
  • Journal Title: Int J Oncol. 37(3) Pages: 695-705
  • Peer Reviewed
• [Journal Article] Establishment of a novel ghrelin-producing cell line. (2010)
  • Author(s): Iwakura H, Li Y, Ariyasu H, Hosoda H, Kanamoto N, Bando M, Yamada G, Hosoda K, Nakao K, Kangawa K, Akamizu T.
  • Journal Title: Endocrinology. 151(6) Pages: 2940-2945
  • Peer Reviewed
• [Journal Article] A postweaning reduction in circulating ghrelin temporarily alters growth hormone (GH) responsiveness to GH-releasing hormone in male mice but does not affect somatic growth. (2010)
  • Author(s): Ariyasu H, Iwakura H, Yamada G, Kanamoto N, Bando M, Kohno K, Sato T, Kojima M, Nakao K, Kangawa K, Akamizu T.
  • Journal Title: Endocrinology. 151(4) Pages: 1743-1750
  • NAID: 120002949719
  • Peer Reviewed
• [Journal Article] Establishment of a novel ghrelin-producing cell line. (2010)
  • Author(s): 岩倉浩
  • Journal Title: Endocrinology Volume: 151 Pages: 2940-5
  • Peer Reviewed
• [Journal Article] Generation of transgenic mice overexpressing a ghrelin analog. (2010)
  • Author(s): 山田豪
  • Journal Title: Endocrinology Volume: 151 Pages: 5935-40
  • Peer Reviewed
• [Journal Article] A postweaning reduction in circulating ghrelin temporarily alters growth hormone (GH) responsiveness to GH-releasing hormone in male mice but does not affect somatic growth. (2010)
  • Author(s): 有安宏之
  • Journal Title: Endocrinology Volume: 151 Pages: 1743-50
  • Peer Reviewed
• [Journal Article] Molecular characterization of tumors from a transgenic mouse adrenal tumor model : comparison with human pheochromocytoma. (2010)
  • Author(s): 服部喜之
  • Journal Title: Int J Oncol Volume: 37 Pages: 695-705
  • Peer Reviewed
• [Journal Article] Ghrelin and functional dyspepsia. (2010)
  • Author(s): 赤水尚史
  • Journal Title: Int J Pept. Volume: 2010 Pages: 548457-548457
• [Journal Article] A mouse model of ghrelinoma exhibited activated growth hormone-insulin-like growth factor I axis and glucose intolerance. (2009)
  • Author(s): Iwakura H, Ariyasu H, Li Y, Kanamoto N, Bando M, Yamada G, Hosoda H, Hosoda K, Shimatsu A, Nakao K, Kangawa K, Akamizu T.
  • Journal Title: Am J Physiol Endocrinol Metab. 297(3) Pages: 802-811
  • Peer Reviewed
• [Journal Article] A mouse model of ghrelinoma exhibited activated growth hormone-insulin-like growth factor I axis and glucose intolerance. (2009)
  • Author(s): 岩倉浩
  • Journal Title: Am J Physiol Endocrinol Metab 297
  • Peer Reviewed
• [Presentation] Establishment of a ghrelin-producing cell line from a gastric tumor in ghrelin-promoter SV40 T-antigen transgenic mice. (2010)
  • Author(s): Iwakura, et al.
  • Organizer: 5th International Peptide Symposium
  • Place of Presentation: Kyoto
  • Year and Date: 2010-12-06
• [Presentation] グレリノーマモデルマウスとグレリン分泌細胞株の樹立と解析 (2010)
  • Author(s): 岩倉浩
  • Organizer: 第15回アディポサイエンス研究会シンポジウム
  • Place of Presentation: 千里新阪急ホテル
  • Year and Date: 2010-08-21
• [Presentation] グレリノーマモデルマウスの作製と解析 (2010)
  • Author(s): 岩倉浩
  • Organizer: 第53回日本糖尿病学会年次学術集会
  • Place of Presentation: 岡山国際交流センター
  • Year and Date: 2010-05-28
• [Presentation] Establishment of a transgenic mouse model of ghrelinoma. (2010)
  • Author(s): Iwakura H, et al.
  • Organizer: the 14th International Congress of Endocrinology (ICE2010)
  • Place of Presentation: Kyoto
  • Year and Date: 2010-03-29
• [Presentation] Establishment of a transgenic mouse model of ghrelinoma (2010)
  • Author(s): 岩倉浩
  • Organizer: The 14th International Congress of Endocrinology
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-03-29
• [Presentation] Establishment of a mouse model of ghrelinoma (2009)
  • Author(s): 岩倉浩
  • Organizer: The 2009 International Symposium on Ghrelin
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2009-11-19
• [Presentation] グレリン投与がヒトの糖代謝に与える影響の検討人工股関節置換周術期患者に対するグレリン無作為二重盲検偽薬対照比較試験より (2009)
  • Author(s): 岩倉浩
  • Organizer: 第52回日本糖尿病学会年次学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-05-21
• [Presentation] グレリン投与がヒトの糖代謝に与える影響の検討 人工股関節置換周術期患者に対するグレリン無作為二重盲検偽薬対照比較試験より (2009)
  • Author(s): 岩倉浩
  • Organizer: 糖尿病学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-05-21
• [Presentation] グレリン細胞腫瘍化トランスジェニックマウスの解析 (2009)
  • Author(s): 岩倉浩
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 前橋商工会議所
  • Year and Date: 2009-04-23
• [Presentation] Effects of Three Weeks Treatment of Ghrelin on Body Composition and Metabolic Parameters in Patients Undergoing Total Hip Replacement for Osteoarthritis. (2009)
  • Author(s): 岩倉浩
  • Organizer: The Endocrine Society's 91st Annual Meeting
  • Place of Presentation: Washington, DC(USA)
• [Presentation] Effects of Three Weeks Treatment of Ghrelin on Body Composition and Metabolic Parameters in Patients Undergoing Total Hip Replacement for Osteoarthritis.
  • Author(s): Iwakura H, et al.
  • Place of Presentation: in Washington, DC