| Project/Area Number |
21790893
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Endocrinology
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
ARIYASU Hiroyuki Kyoto University, 医学研究科, 助教 (50378650)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | 成長ホルモン / グレリン / GH(成長ホルモン) |
|---|
| Research Abstract |
In this study, we generated a transgenic mouse that expresses human diphtheria toxin (DT) receptor cDNA in ghrelin-secretion cells (GPDTR-Tg mice). Administration of DT to this mouse ablates ghrelin-secretion cells in a controlled manner. Four days after injection of DT into GPDTR-Tg mice, ghrelin-secreting cells were ablated, and plasma levels of ghrelin were markedly decreased. Daily food intake of these animals, however, was not affected. Three-week-old GPDTR-Tg mice were then treated with DT twice a week for five weeks. Although plasma ghrelin levels were reduced, GPDTR-Tg mice did not display any decreases in serum GH or IGF-1 levels or any growth retardation. Our results strongly suggest that circulating ghrelin does not play a crucial role in either feeding behavior or somatic growth.
|
