| Project/Area Number |
21790895
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Endocrinology
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| Research Institution | Nagasaki University |
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Principal Investigator |
ROGOUNOVITCH Tatiana Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (30423643)
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|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
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| Keywords | 甲状腺乳頭癌 / 変異BRAF / レトロウイルスマッピング / レトロウィルスマッピング |
|---|
| Research Abstract |
Activation of oncogene alone is usually insufficient to transform epithelial cells. For PTCs driven by mutant BRAF we attempted to reveal the additional genetic abnormalities that are required to confer malignant phenotype on cancer cells. For this purpose, as a model, we performed retrovirus insertional mutagenesis in doxycycline-inducible BRAFV600E-expressing clonal lines derived from rat thyroid PCCL3 cells, and picked the clones that do not require TSH for growth. By PCR-based splinkerette procedure and microarray analysis we have successfully identified several possible candidate genes (Trpm1, Nek6, etc).
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