Research for physiological role of desacyl ghrelin by functional analysis of its receptor endogenously expressed in cultured cells

• Project/Area Number: 21790901
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: MORI Miwa 独立行政法人国立循環器病研究センター, 生化学部, 流動研究員 (50363148)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: グレリン / デスアシルグレリン / 受容体 / 内分泌

Research Abstract

The orexigenic hormone ghrelin has two major molecular forms : acylated ghrelin and desacyl ghrelin. Intracerebroventricularly administered desacyl ghrelin induces food intake in rodent by a mechanism independent of receptor for acylated ghrelin. However, desacyl ghrelin receptor is not yet identified. In this study, the cell lines endogenously expressing receptor for desacyl ghrelin were investigated. Desacyl ghrelin induced cAMP production in H9c2 and C2C12 cells in dose-dependent manner, suggesting that desacyl ghrelin receptor may be expressed in these cell lines.