Molecular Pathogenesis of Primary Myelofibrosis Mouse

• Project/Area Number: 21790909
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: KANEKO Shin The University of Tokyo, 医科学研究所, 助教 (40361331)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 血液腫瘍学 / 癌幹細胞生物学 / 骨髄増殖性腫瘍 / 原発性骨髄線維症 / 造血幹細胞 / 疾患モデル動物 / STAT5A / 真性多血症 / 間葉系幹細胞 / STAT5 / c-Myc / 骨髄増殖性疾患 / JAK2V617F変異 / レトロウイルスベクター / 疾患モデルマウス

Research Abstract

We have developed a model mouse of primary myelofibrosis using genetically modified hematopoietic stem cell transplantation. Progression manner and molecular pathogenesis of bone marrow fibrosis in the mouse have closely resembled to those of the human disease. Using the mouse model, we have demonstrated that normal HSCs also can be affected to lose stem cell potentials in fibrotic marrow. The newly established mouse model would be useful for developing new molecular therapies against primary myelofibrosis.

Research Products

• [Journal Article] Constitutive STAT5A activation enables erythropoiesis from human embryonic stem cell-derived primitive hematopoietic cells in the absence of erythropoietin (2011)
  • Author(s): Shimizu T, Sano S, Takayama N, Kaneko S (corresponding author), Eto K, Takeuchi Y, Nakauchi H.
  • Journal Title: The Kitasato Medical Journal 41 Pages: 42-49
  • Peer Reviewed
• [Journal Article] Constitutive STAT5A activation enables erythropoiesis from human embryonic stem cell-derived primitive hematopoietic cells in the absence of erythropoietin (2011)
  • Author(s): Takafumi Shimizu
  • Journal Title: The Kitasato Medical Journal Volume: 41 Pages: 42-49
  • Peer Reviewed
• [Presentation] Developing Mouse Model of Myeloproliferative Neoplasms by Genetically Modified Highly Purified Hematopoietic Stem Cell Transplantation (2011)
  • Author(s): Kaneko S
  • Organizer: USA-Japan Cooperative Cancer Research Workshop
  • Place of Presentation: Hayama, Japan
  • Year and Date: 2011-02-25
• [Presentation] Developing Mouse Model of Myeloproliferative Neoplasms by Genetically Modified Highly Purified Hematopoietic Stem Cell Transplantation (2011)
  • Author(s): Shin Kaneko
  • Organizer: 日米血液腫瘍セミナー2011
  • Place of Presentation: 葉山
  • Year and Date: 2011-02-25
• [Presentation] STAT5A over-expression induces hematopoietic exhaustion not only in transduced hematopoietic stem cells but also to non-transduced normal hematopoietic stem cells (2010)
  • Author(s): Shimizu T, Kaneko S, et al
  • Organizer: European School of Hematology, International Conference; Myeloproliferative Neoplasms
  • Place of Presentation: Albufeira, Portugal
  • Year and Date: 2010-10-02
• [Presentation] STAT5A over-expression induces hematopoietic exhaustion not only in transduced hematopoietic stem cells but also to non-transduced normal hematopoietic stem (2010)
  • Author(s): Takafumi Shimizu
  • Organizer: European School of Hematology, International Conference ; Myeloproliferative Neoplasms
  • Place of Presentation: Albufeira, Portugal
  • Year and Date: 2010-10-02
• [Presentation] Analysis of hematopoiesis in advanced PMF mice generated by STAT5A(1*6) HSC transplantation (2010)
  • Author(s): Shimizu T, Kaneko S, et al
  • Organizer: 第72回日本血液学会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-26
• [Presentation] Analysis of hematopoiesis in advanced PMF mice generated by STAT5A (1^*6) HSC transplantation (2010)
  • Author(s): Takafumi Shimizu
  • Organizer: 第72回日本血液学会総会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2010-09-26
• [Presentation] 遺伝子治療・再生医療の現状と可能性 (2010)
  • Author(s): 金子新
  • Organizer: 関東甲信地区遺伝子・染色体検査セミナー
  • Place of Presentation: 東京
  • Year and Date: 2010-03-14
• [Presentation] Establishment of mouse PMF model by introduction of constitutive STAT5a into purified hematopoietic stem cells (2009)
  • Author(s): Shimizu T, Kaneko S, et al
  • Organizer: Annual meeting of American Society of Hematology
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2009-12-06
• [Presentation] Establishment of mouse PMF model by introduction of constitutive STAT5a into purified hematopoietic stem cells (2009)
  • Author(s): 清水崇史
  • Organizer: 米国血液学会
  • Place of Presentation: ニューオーリンズ・米国
  • Year and Date: 2009-12-06
• [Presentation] STAT5a活性化造血幹細胞に起因する原発性骨髄線維症モデルマウスの作製および解析 (2009)
  • Author(s): 清水崇史, 金子新, ら
  • Organizer: 第71回日本血液学会
  • Place of Presentation: 京都
  • Year and Date: 2009-10-24
• [Presentation] STAT5a活性化造血幹細胞に起因する原発性骨髄線維症モデルマウスの作製および解析 (2009)
  • Author(s): 清水崇史
  • Organizer: 日本血液学会
  • Place of Presentation: 京都
  • Year and Date: 2009-10-24
• [Remarks] ホームページ
  • URL: http://stemcell-u-tokyo.org/sct/project/
• [Remarks]
  • URL: http://stemcell-u-tokyo.org/sct/project/#anchor04
• [Remarks]
  • URL: http://stemcell-u-tokyo.org/sct/project/