Analysis of hematopoietic stem cell and leukemic stem cell niche by focusing on Spred-1 functions

• Project/Area Number: 21790910
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Kanazawa University
• Principal Investigator: TADOKORO Yuko Kanazawa University, がん研究所, 助教 (00447343)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 幹細胞 / 白血病幹細胞 / 幹細胞ニッチ / がん

Research Abstract

The aim of this research project is to understand both the similarities and differences between hematopoietic stem cells and leukemic stem cells and their niches. I have carried out competitive repopulation assay using Spred-1 knockout and leukemia-developed mouse bone marrow cells. Leukemic stem cells showed cell surface marker phenotype and multipotency similar to that of hematopoietic stem cells. Furthermore, competitive repopulation assay with Spred-1 knockout bone marrow cells suggested that both stem cells might compete in their sharing niches. However, Spred-1 knockout cells could not significantly rescue the leukemia-developed mice.

Research Products

• [Journal Article] Nishimura.Hair Follicle Stem Cells Provide a Functional Niche for Melanocyte Stem Cells. (2011)
  • Author(s): Shintaro Tanimura, Yuko Tadokoro(co-first author), Ken Inomata, Nguyen Thanh Binh, Wataru Nishie, Satoshi Yamazaki, Hiromitsu Nakauchi, Yoshio Tanaka, James R.McMillan, Daisuke Sawamura, Kim Yancey, Hiroshi Shimizu, Emi K.
  • Journal Title: Cell Stem Cell 8(2) Pages: 177-187
  • Peer Reviewed
• [Journal Article] NKX2.2Suppresses Self-Renewal of Glioma-Initiating Cells. (2011)
  • Author(s): Teruyuki Muraguchi, Shingo Tanaka, Daisuke Yamada, Akira Tamase, Mitsutoshi Nakada, Hideo Nakamura, Takayuki Hoshii, Takako Ooshio, Yuko Tadokoro, Kazuhito Naka, Yasushi Ino, Tomoki Todo, Jun-ichi Kuratsu, Hideyuki Saya, Jun-ichiro Hamada, Atsushi Hirao
  • Journal Title: Cancer Research 71(3) Pages: 1135-1145
  • Peer Reviewed
• [Journal Article] Hair Follicle Stem Cells Provide a Functional Niche for Melanocyte Stem Cells (2011)
  • Author(s): Shintaro Tanimura
  • Journal Title: Cell Stem Cell Volume: 8 Pages: 177-187
  • Peer Reviewed
• [Journal Article] NKX2.2 Suppresses Self-Renewal of Glioma-Initiating Cells (2011)
  • Author(s): Teruyuki Muraguchi
  • Journal Title: Cancer Research Volume: 71 Pages: 1135-1145
  • Peer Reviewed
• [Journal Article] TGF-β.FOXO signalling maintains leukaemia-initiating cells in chronic myeloid leukaemia. (2010)
  • Author(s): Kazuhito Naka, Takayuki Hoshii, Teruyuki Muraguchi, Yuko Tadokoro, Takako Ooshio, Yukio Kondo, Shinji Nakao, Noboru Motoyama, Atsushi Hirao
  • Journal Title: Nature 463 Pages: 676-680
  • Peer Reviewed
• [Journal Article] TGF-β-FOXO signalling maintains leukaemia-initiating cells in chronic myeloid leukaemia (2010)
  • Author(s): Kazuhito Naka
  • Journal Title: Nature 463 Pages: 676-680
  • Peer Reviewed
• [Presentation] Enhanced competitive repopulation ability of hematopoietic stem cells by inhibition of Spred-1. (2011)
  • Author(s): Yuko Tadokoro
  • Organizer: USA-Japan Cooperative Cancer Research Workshop
  • Place of Presentation: 湘南国際村センター(神奈川県)
  • Year and Date: 2011-02-26
• [Presentation] Enhanced competitive repopulation ability of hematopoietic stem cells by inhibition of Spred-1 (2011)
  • Author(s): Yuko Tadokoro
  • Organizer: USA-Japan Cooperative Cancer Research Workshop
  • Place of Presentation: 湘南国際村センター(神奈川県)
  • Year and Date: 2011-02-26
• [Book] 幹細胞研究の最近の進歩(後篇)組織幹細胞「毛包における幹細胞とそのニッチ」 (2009)
  • Author(s): 田所優子, 西村栄美
  • Publisher: 最新医学社
• [Book] 幹細胞研究の最近の進歩(後篇)組織幹細胞 (2009)
  • Author(s): 田所優子
  • Publisher: 最新医学社