Analysis of the role of ETV6/FLT3 in oncogenesis, using a murine bone marrow transduction model

• Project/Area Number: 21790913
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Kyoto University
• Principal Investigator: HISHIZAWA Masakatsu Kyoto University, 大学院・医学研究科, 助教 (90444455)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 血液腫瘍学 / 白血病 / マウス / 融合遺伝子 / FLT3 / ETV6

Research Abstract

We identified a novel ETV6/FLT3 variant fusion transcript in myeloproliferative disease (MPD) with T-lymphoblastic leukemia/lymphoma (T-LBL). To investigate the role of ETV6/FLT3 fusion gene in oncogenesis, we used a murine bone marrow transplant model. ETV6/FLT3 induced a rapidly fatal MPD in primary recipient mice. We demonstrated that the Grb2-Gab2 pathway plays an important role in oncogenesis.

Research Products

• [Journal Article] Transplantation of allogeneic hematopoietic stem cells for adult T-cell leukemia : a nationwide retrospective study (2010)
  • Author(s): Hishizawa Masakatsu, et.al.
  • Journal Title: Blood Volume: 116 Pages: 1369-1376
  • Peer Reviewed
• [Journal Article] Transfusion-Related Acute Lung Injury (TRALI) Induced by Donor-Derived Anti-HLA Antibodies in Aplastic Anemia : Possible Priming Effect of Granulocyte-Colony Stimulating Factor (G-CSF) on the Recipient Neutrophils (2009)
  • Author(s): Masakatsu Hishizawa, et.al.
  • Journal Title: Internal Medicine 48 Pages: 1979-1983
  • NAID: 130000122222
  • Peer Reviewed
• [Journal Article] 成人T細胞白血病 (2009)
  • Author(s): 菱澤方勝、内山卓
  • Journal Title: 医学と薬学 61 Pages: 163-169
• [Presentation] Molecular cloning and functional analysis of PCM1/JAK2 fusion gene in MPN with t(8;9)(p22;p24) (2010)
  • Author(s): Chonabayashi Kazuhisa, et al.
  • Organizer: 第72回日本血液学会学術総会
  • Place of Presentation: パシフィコ横浜、横浜
• [Presentation] Direct Binding of Grb2 Is Required for Efficient Induction of Myeloproliferative Disease in Mice by ETV6/FLT3 (2009)
  • Author(s): Kazuhisa Chonabayashi, et.al.
  • Organizer: 51th American Society of Hematology Annual Meeting and Exposition
  • Place of Presentation: Ernest N.Morial Convention Center, New Orleans, LA, U.S.
  • Year and Date: 2009-12-06
• [Presentation] ETV6/FLT3 fusion gene induces a fatal myeloproliferative disorder in a murine rbone marrow transplantation model (2009)
  • Author(s): Kazuhisa Chonabayashi, et.al.
  • Organizer: 第71回日本血液学会学術総会
  • Place of Presentation: 京都国際会館、京都
  • Year and Date: 2009-10-24
• [Presentation] Efficient and rapid induction of myeloproliferative disorder (MPD) by ETV6/FLT3 fusion gene using a murine bone marrow transplant model (2009)
  • Author(s): Kazuhisa Chonabayashi, et.al.
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2009-10-02
• [Presentation] Direct Binding of Grb2 Is Required for Efficient Induction of Myeloproliferative Disease in Mice by ETV6/FLT3. (2009)
  • Author(s): Kazuhisa Chonabayashi, Masakatsu Hishizawa, Shin Kawamata, Masashi Matsui, Tatsuharu Ohno, Takayuki Ishikawa, Takashi Uchiyama.
  • Organizer: 51th American Society of Hematology Annual Meeting and Exposition
  • Place of Presentation: New Olreans, USA.
• [Presentation] Efficient and rapid induction of myeloproliferative disorder (MPD) by ETV6/FLT3 fusion gene using a murine bone marrow transplant model. (2009)
  • Author(s): Kazuhisa Chonabayashi, Masakatsu Hishizawa, Takayuki Ishikawa, Takashi Uchiyama.
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] ETV6/FLT3 fusion gene induces a fatal myeloproliferative disorder in a murine rbone marrow transplantation model. (2008)
  • Author(s): Kazuhisa Chonabayashi, Masakatsu Hishizawa, Shin Kawamata, Tatsuharu Ohno, Takayuki Ishikawa, Takashi Uchiyama.
  • Organizer: 第71回日本血液学会学術総会
  • Place of Presentation: 京都
• [Remarks] ホームページ等