Vinculin is indispensable for hematopoietic stem cell repopulation
| Project/Area Number |
21790920
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
OHMORI Tsukasa Jichi Medical University, 医学部, 講師 (70382843)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 造血幹細胞移植学 / インテグリン / 造血幹細胞 / Talin / 細胞骨格 |
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| Research Abstract |
Vinculin is a highly conserved actin-binding protein that is localized in integrin-mediated focal adhesion complexes. This study showed that the ability of c-Kit+Sca1+Lin- (KSL) hematopoietic stem cells (HSCs) to support reconstitution of hematopoiesis after competitive transplantation was severely impaired by lentiviral transduction with short hairpin RNA sequences for vinculin, albeit adhesion to the extracellular matrix and homing to bone marrow were not inhibited. Our results suggest that vinculin is an indispensable factor determining HSC repopulation capacity, independent of integrin functions.
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Report
(3 results)
Research Products
(20 results)
