Cotransplantation with MSCs improves engraftment of HSCs after autologous intra-bone marrow transplantation in nonhuman primates
| Project/Area Number |
21790922
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
MASUDA Shigeo Jichi Medical University, 医学部, 講師 (10396749)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 霊長類 / サル / 造血幹細胞 / 間葉系幹細胞 / 骨髄内移植 / ニッチ / 生着 / 造血幹細胞ニッチ / 骨芽細胞性ニッチ |
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| Research Abstract |
Hematopoietic stem cells (HSCs) reside in the osteoblastic niche, which consists of osteoblasts derived from mesenchymal stem cells (MSCs). Here, using nonhuman primates, we investigated the effects of cotransplantation with MSCs on the engraftment of genetically marked CD34^+ cells after autologous intra-bone marrow transplantation (iBMT). In three cynomolgus monkeys, CD34^+ cells transplanted with MSCs engrafted 4.4-, 6.0-, and 1.6-times more efficiently than CD34^+ cells alone, as assessed by bone marrow (BM) colony PCR. In addition, virtually all of the marked cells detected in the peripheral blood (PB) were derived from the cotransplantation aliquots. Notably, colony-forming units (CFUs) derived from the cotransplantation aliquots were frequently detected in BM, distant from the injection site. Thus, cotransplantation with MSCs would improve the efficacy of transplantation of gene-modified HSCs in primates, with enhanced engraftment in BM as well as increased chimerism in PB through migration and homing.
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Report
(3 results)
Research Products
(53 results)
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[Journal Article] Dual antitumor mechanisms of Notch signaling inhibitor in a T-cell acute lymphoblastic leukemia xenograft model.2009
Author(s)
Masuda S, Kumano K, Suzuki T, Tomita T, Iwatsubo T, Natsugari H, Tojo A, Shibutani M, Mitsumori K, Hanazono Y, Ogawa S, Kurokawa M, Chiba S.
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Journal Title
Cancer Sci. 100(12)
Pages: 2444-2450
NAID
Related Report
Peer Reviewed
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[Journal Article] Notch activation induces the generation of functional natural killer cells from human cord blood CD34-positive cells devoid of interleukin-15.2009
Author(s)
Haraguchi K, Suzuki T, Koyama N, Kumano K, Nakahara F, Matsumoto A, Yokoyama Y, Sakata-Yanagimoto M, Masuda S, Takahashi T, Kamijo A, Takahashi K, Takanashi M, Okuyama Y, Yasutomo K, Sakano S, Yagita H, Kurokawa M, Ogawa S, Chiba S.
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Journal Title
J Immunol. 182(10)
Pages: 6168-6178
Related Report
Peer Reviewed
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[Journal Article] ERas is expressed in primate embryonic stem cells but not related to tumorigenesis.2009
Author(s)
Tanaka Y, Ikeda T, Kishi Y, Masuda S, Shibata H, Takeuchi K, Komura M, Iwanaka T, Muramatsu S, Kondo Y, Takahashi K, Yamanaka S, Hanazono Y.
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Journal Title
Cell Transplant. 18(4)
Pages: 381-389
Related Report
Peer Reviewed
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