| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Research Abstract |
Hematopoietic stem cells (HSCs) reside in the osteoblastic niche, which consists of osteoblasts derived from mesenchymal stem cells (MSCs). Here, using nonhuman primates, we investigated the effects of cotransplantation with MSCs on the engraftment of genetically marked CD34^+ cells after autologous intra-bone marrow transplantation (iBMT). In three cynomolgus monkeys, CD34^+ cells transplanted with MSCs engrafted 4.4-, 6.0-, and 1.6-times more efficiently than CD34^+ cells alone, as assessed by bone marrow (BM) colony PCR. In addition, virtually all of the marked cells detected in the peripheral blood (PB) were derived from the cotransplantation aliquots. Notably, colony-forming units (CFUs) derived from the cotransplantation aliquots were frequently detected in BM, distant from the injection site. Thus, cotransplantation with MSCs would improve the efficacy of transplantation of gene-modified HSCs in primates, with enhanced engraftment in BM as well as increased chimerism in PB through migration and homing.
|
