Understanding of significance of angiogenesis/hypoxia-inducible transcription factors and development of novel therapy for rheumatoid arthritis

Research Project

Project/Area Number	21790932
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	膠原病・アレルギー・感染症内科学
Research Institution	Asahikawa Medical College
Principal Investigator	OKAMOTO Kensaku Asahikawa Medical College, 医学部, 助教 (80396879)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	リウマチ学 / 血管新生 / 関節リウマチ / 低酸素 / HIF
Research Abstract	Hypoxia and iron chelator or CoCl2 dependent HIF-1α protein stabilization in time dependent manner was confirmed in mouse-derived cells. Establishment of a constuct expressing HIF-1α specific siRNA is continued. Quantitative analyses of HIF-1 target genes were carried out by real-time PCR. Expression system by Adenovirus vector for siRNA knocking down HIF-1α, or expressing constitutive active HIF-1α, and dominant negative HIF-1α is under construction.

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