Function analysis of autoantigens in human mature osteoclast from rheumatoid arthritis patient under anti-rheumatic drugs.

• Project/Area Number: 21790946
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Kyoto University
• Principal Investigator: NOJIMA Takaki Kyoto University, 医学研究科, 助教 (30327514)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 破骨細胞 / 自己抗原 / 関節リウマチ

Research Abstract

In osteoclastgenesis system using human peripheral-blood mononuclear cells, we found that calpastatin was cut off and calpain revitalization was elevated from 3 day to 6 day. The protein level changes of calpain 1 and calpain 2 were not detected in our osteoclastgenesis system. Cell permeable calpastatin down-regulated the osteoclastgenesis and the bone absorptivity of human osteoclast. After 15 minutes TNFα (100ng/ml) stimulation, calpain revitalization elevated in human osteoclast.

Research Products

• [Journal Article] タクロリムス(ループス腎炎以外のSLE、多発性筋炎/皮膚筋炎、血管炎症候群、成人スティル病など) (2011)
  • Author(s): 野島崇樹
  • Journal Title: リウマチ科 第45巻第1号 Pages: 25-28
• [Journal Article] タクロリムス(ループス腎炎以外のSLE、多発性筋炎/皮膚筋炎、血管炎症候群、成人スティル病など) (2011)
  • Author(s): 野島崇樹
  • Journal Title: リウマチ科 Volume: 45(1) Pages: 25-28
• [Journal Article] Anti-citrullinated peptide antibody-negative RA is a genetically distinct subset : a definitive study using only bone-erosive ACPA-negative rheumatoid arthritis. (2010)
  • Author(s): Ohmura K, Terao C, Maruya E, Katayama M, Matoba K, Shimada K, Murasawa A, Honjo S, Takasugi K, Tohma S, Matsuo K, Tajima K, Yukawa N, Kawabata D, Nojima T, Fujii T, Yamada R, Saji H, Matsuda F, Mimori T.
  • Journal Title: Rheumatology (Oxford) 49(12) Pages: 2298-2304
  • Peer Reviewed
• [Journal Article] Anti-U1 RNP antibodies in cerebrospinal fluid are associated with central neuropsychiatric manifestations in systemic lupus erythematosus and mixed connective tissue disease. (2010)
  • Author(s): Sato T, Fujii T, Yokoyama T, Fujita Y, Imura Y, Yukawa N, Kawabata D, Nojima T, Ohmura K, Usui T, Mimori T.
  • Journal Title: Arthritis Rheum. 62(12) Pages: 3730-3740
  • NAID: 120005593598
  • Peer Reviewed
• [Journal Article] Two cases of late-onset drug-induced lupus erythematosus caused by ticlopidine in elderly men. (2010)
  • Author(s): Yokoyama T, Usui T, Kiyama K, Nakashima R, Yukawa N, Kawabata D, Nojima T, Ohmura K, Fujii T, Mimori T.
  • Journal Title: Mod Rheumatol. 20(4) Pages: 405-409
  • NAID: 10026691598
  • Peer Reviewed
• [Journal Article] Periodic fever and erythema nodosum associated with MDS with trisomy 8 : report of two cases and review of the literature. (2010)
  • Author(s): Fujimura T, Yukawa N, Nakashima R, Imura Y, Kawabata D, Nojima T, Ohmura K, Fujii T, Usui T, Mimori T.
  • Journal Title: Mod Rheumatol. 20(4) Pages: 413-419
  • NAID: 10026691630
  • Peer Reviewed
• [Journal Article] Discontinuation of infliximab after attaining low disease activity in patients with rheumatoid arthritis : RRR (remission induction by Remicade in RA) study. RRR study investigators. (2010)
  • Author(s): Tanaka Y, Takeuchi T, Mimori T, Saito K, Nawata M, Kameda H, Nojima T, Miyasaka N, Koike T
  • Journal Title: Ann Rheum Dis. 69(7) Pages: 1286-1291
  • Peer Reviewed
• [Journal Article] Etanercept-induced anti-Jo-1-antibody-positive polymyositis in a patient with rheumatoid arthritis : a case report and review of the literature. (2010)
  • Author(s): 石川優樹, Yukawa N, Ohmura K, Hosono Y, Imura Y, Kawabata D, Nojima T, Fujii T, Usui T, Mimori T.
  • Journal Title: Clin Rheumatol. 29(5) Pages: 563-566
  • NAID: 120002191883
  • Peer Reviewed
• [Journal Article] The RIG-I-like receptor IFIH1/MDA5 is a dermatomyositis-specific autoantigen identified by the anti-CADM-140 antibody. (2010)
  • Author(s): Nakashima R, Imura Y, Kobayashi S, Yukawa N, Yoshifuji H, Nojima T, Kawabata D, Ohmura K, Usui T, Fujii T, Okawa K, Mimori T.
  • Journal Title: Rheumatology (Oxford) 49(3) Pages: 433-340
  • Peer Reviewed
• [Journal Article] 関節炎におけるinterleukin-1 receptor-associated kinase-4の役割(解説) (2010)
  • Author(s): 石川優樹, 野島崇樹
  • Journal Title: リウマチ科 44巻1号 Pages: 69-74
• [Journal Article] 【関節リウマチ(第2版)寛解を目指す治療の新時代】関節リウマチの検査・診断免疫・血清学的検査細胞性免疫検査(解説/特集) (2010)
  • Author(s): 野島崇樹
  • Journal Title: 日本臨床 68巻増刊号5関節リウマチ Pages: 258-261
• [Journal Article] ミニレクチャー:関節リウマチでのメトトレキサートの使い方 (2010)
  • Author(s): 野島崇樹
  • Journal Title: 臨床研修プラクティス 7巻3号 Pages: 73-74
• [Journal Article] Anti-citrullinated peptide antibody-negative RA is a genetically distinct subset : a definitive study using only bone-erosive ACPA-negative rheumatoid arthritis. (2010)
  • Author(s): Ohmura K, Terao C, Nojima T, et al
  • Journal Title: Rheumatology (Oxford) Volume: 49(12) Pages: 2298-2304
  • Peer Reviewed
• [Journal Article] Anti-U1 RNP antibodies in cerebrospinal fluid are associated with central neuropsychiatric manifestations in systemic lupus erythematosus and mixed connective tissue disease. (2010)
  • Author(s): Sato T, Fujii T, Nojima T, et al
  • Journal Title: Arthritis Rheum Volume: 62(12) Pages: 3730-3740
  • NAID: 120005593598
  • Peer Reviewed
• [Journal Article] Discontinuation of infliximab after attaining low disease activity in patients with rheumatoid arthritis : RRR (remission induction by Remicade in RA) study (2010)
  • Author(s): Tanaka Y, Takeuchi T, Nojima T, et al
  • Journal Title: Ann Rheum Dis Volume: 69(7) Pages: 1286-1291
  • Peer Reviewed
• [Journal Article] Etanercept-induced anti-Jo-1-antibody-positive polymyositis in a patient with rheumatoid arthritis : a case report and review of the literature. (2010)
  • Author(s): Ishikawa Y, Yukawa N, Nojima T, et al
  • Journal Title: Clin Rheumatol Volume: 29(5) Pages: 563-566
  • NAID: 120002191883
  • Peer Reviewed
• [Journal Article] The RIG-I-like receptor IFIH1/MDA5 is a dermatomyositis-specific autoantigen identified by the anti-CADM-140 antibody. (2010)
  • Author(s): Nakashima R, Imura Y, Nojima T, et al
  • Journal Title: Rheumatology (Oxford) 49(3) Pages: 433-440
  • Peer Reviewed
• [Journal Article] Gamma/delta T cells are the predominant source of interleukin-17 in affected joints in collagen-induced arthritis, but not in rheumatoid arthritis. (2009)
  • Author(s): Ito Y, Usui T, Kobayashi S, Iguchi-Hashimoto M, Ito H, Yoshitomi H, Nakamura T, Shimizu M, Kawabata D, Yukawa N, Hashimoto M, Sakaguchi N, Sakaguchi S, Yoshifuji H, Nojima T, Ohmura K, Fujii T, Mimori T.
  • Journal Title: Arthritis Rheum. 60(8) Pages: 2294-2303
  • Peer Reviewed
• [Journal Article] マクロファージにおけるAMP-activated protein kinaseの抗炎症作用 (2009)
  • Author(s): 野島崇樹
  • Journal Title: リウマチ科 第42巻第4号 Pages: 424-428
• [Journal Article] 早期診断と鑑別診断の留意点 (2009)
  • Author(s): 野島崇樹
  • Journal Title: リウマチ科 第41巻第6号 Pages: 553-557
• [Journal Article] 早期診断と鑑別診断の留意点 (2009)
  • Author(s): 野島崇樹
  • Journal Title: リウマチ科 41(6) Pages: 553-557
• [Journal Article] (2009)
  • Author(s): 野島崇樹
  • Journal Title: リウマチ科 42(4) Pages: 424-428
• [Journal Article] Gamma/delta T cells are the predominant source of interleukin-17 in affected joints in collagen-induced arthritis, but not in rheumatoid arthritis. (2009)
  • Author(s): Ito Y, Usui T, Nojima T, et al
  • Journal Title: Arthritis Rheum. 60(8) Pages: 2294-2303
  • Peer Reviewed
• [Presentation] 内科的治療の変遷-生物学的製剤を中心に- (2009)
  • Author(s): 野島崇樹
  • Organizer: 第36回日本股関節学会学術集会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2009-10-30
• [Presentation] 内科的治療の変遷-生物学的製剤を中心に- (2009)
  • Author(s): 野島崇樹
  • Organizer: 第36回 日本股関節学会学術集会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2009-10-30
• [Book] 研修医のための膠原病診療ガイド(関節リウマチでのメトトレキサートの使い方(解説/特集))(7巻3号) (2010)
  • Author(s): 野島崇樹
  • Publisher: 臨床研修プラクティス
• [Book] リウマトイド因子と抗核抗体(リウマチ・膠原病内科クリニカルスタンダード必携ベッドサイドで必ず役立つリウマチ・膠原病学のエッセンス) (2010)
  • Author(s): 石川優樹, 野島崇樹
  • Publisher: 文光堂