In vitro analyses of the pathogenesis of hypertrophic cardiomyopathy by using cardiomyocyte differentiation system

• Project/Area Number: 21790985
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: Osaka University
• Principal Investigator: OKADA Yoko Osaka University, 大学院・医学系研究科, 寄附講座助教 (30457022)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 心筋症 / 心筋細胞 / 分化誘導 / 肥大シグナル / レオパルド症候群

Research Abstract

The mechanisms of cardiac hypertrophy associated with LEOPARD syndrome were analyzed by using P19CL6 cells, which is a convenient cardiomyocyte differentiation model in vitro. The LEOPARD-type mutant SHP2 attenuated the terminal differentiation of cardiomyocyte with increased proliferative activity, as compared to the controls and Noonan-type SHP2 mutant. It also induced cardiomyocyte hypertrophy. These alterations were associated with the dysregulation of Akt/GSk3β/β-catenin pathway.

Research Products

• [Presentation] LEOPARD type SHP2 mutant Q510E attenuates cardiomyocyte differentiation via aberrant β-catenin signaling and promotes cardiomyocyte hypertrophy by PI3K/Akt pathway (2011)
  • Author(s): Ishida H, Okada Y, Ichimori H, Narita J, Kogaki S, Ozono K
  • Organizer: Keystone Symposia Mechanisms of Cardiac Growth, Death, and Regeneration 2011
  • Place of Presentation: Keystone, Colorado, USA
  • Year and Date: 2011-02-23
• [Presentation] LEOPARD type SHP2 mutant Q510E attenuates cardiomyocyte differentitation via aberrant β-catenin signaling and promotes cardiomyocyte hypertrophy by PI3K/Akt pathway. (2011)
  • Author(s): Ishida H, Okada Y, Ichimori H, Narita J, Kogaki S, Ozono K
  • Organizer: Keystone Synposia Mechanisms of Cardiac Growth, Death, and Regeneration 2011
  • Place of Presentation: Keystone Colorado USA
  • Year and Date: 2011-02-06
• [Presentation] 心筋細胞分化誘導システムを用いたLEOPARD症候群における肥大型心筋症発症機構の解明 (2010)
  • Author(s): 石田秀和, 岡田陽子, 市森裕章, 成田淳, 小垣滋豊, 大薗恵一
  • Organizer: 日本小児循環器学会2010
  • Place of Presentation: 東京
• [Presentation] 心筋細胞分化誘導システムを用いたLEOPARD症候群における肥大型心筋症発症機構の解明 (2010)
  • Author(s): 石田秀和, 岡田陽子, 市森裕章, 成田淳, 小垣滋豊, 大薗恵一
  • Organizer: 日本小児循環器学会総会2010
  • Place of Presentation: 東京