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Therapeutic application to neonatal PVL of amnion-derived cells

Research Project

Project/Area Number 21791029
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Embryonic/Neonatal medicine
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

SASAKI Yoshihito  独立行政法人国立循環器病研究センター, 周産期・婦人科部 (50396389)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords胎児医学 / 羊膜細胞 / SP細胞 / リアルタイムPCR
Research Abstract

We have obtained human amnion during caesarian section from 20 volunteers. After the culture, amniotic cells were sorted by flow cytometry. The cell fraction that we consider containing stem cells is no more than 0.02% of total cells. A neonatal rat model with hypoxic-ischemic brain injury was prepared. We injected these cells suspended in a solution into the lateral ventricle of the brain. We also applied the cells as a form of cell sheet directly onto the injured cerebral cortex. Neuroprotective effects of the cell therapies have not been observed so far. We continue analyzing other methods of cell delivery and other cell sources.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (1 results)

All 2008

All Presentation (1 results)

  • [Presentation] Intracranial Injection of Fetal Membranous Mesenchymal Stem Cells Following Hypoxic Ischemic Encephalopathy in Neonatal Rats.2008

    • Author(s)
      Keiko Ueda, Kazuhiko Harada, Jun Yoshimatu, Shinji Kasturagi, Shin Ishikane, Noritoshi Nagaya, Tomoaki Ikeda
    • Organizer
      6^<TH> Hershey Conference on Developing Brain Injury
    • Place of Presentation
      Paris France
    • Related Report
      2010 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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